کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902290 | 1545798 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design and selection of an intrabody library produced de-novo for the non-structural protein NSP5 of rotavirus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Design and selection of an intrabody library produced de-novo for the non-structural protein NSP5 of rotavirus Design and selection of an intrabody library produced de-novo for the non-structural protein NSP5 of rotavirus](/preview/png/9902290.png)
چکیده انگلیسی
Intracellular antibodies or intrabodies have great potential in protein knockout strategies for intracellular antigens. We applied the Intracellular Antibody Capture Technology for the direct selection in yeast of a mouse scFv library (VL-VH format) constructed from animals immunised with recombinant non-structural protein NSP5 of Rotavirus. We selected five different intracellular antibodies (ICAbs), which specifically recognize Î2, an NSP5 deletion mutant used as bait. The anti-NSP5 ICAbs were well expressed both in yeast and mammalian cells as cytoplasmic or nuclear-tagged forms. By immunofluorescence and co-immunoprecipitation assays we characterised the intracellular interaction of the five anti-NSP5 ICAbs with the co-expressed antigens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 301, Issues 1â2, June 2005, Pages 31-40
Journal: Journal of Immunological Methods - Volume 301, Issues 1â2, June 2005, Pages 31-40
نویسندگان
Fulvia Vascotto, Michela Visintin, Antonino Cattaneo, Oscar R. Burrone,