کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902377 | 1545802 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of an assay for the quantification of type I collagen synthesis in the guinea pig
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کلمات کلیدی
SDSmAbPICPCompetition ELISAMSXmIgGGPPTMBMonoclonal antibody - آنتی بادی مونوکلونالELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاSurface plasmon resonance - تشدید پلاسمون سطحیSPR - تشدید پلاسمون سطحیGuinea pig - خوک گینه، خوکچه هندیRam - رمcolumn volumes - ستون حجمsodium dodecyl sulphate - سدیم دودسیل سولفاتresponse units - واحدهای پاسخ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
There is a need for a reliable assay for the quantification of collagen type I synthesis in the guinea pig, an important model for many connective tissue diseases. Procollagen type I C-terminal propeptide (PICP) is the established marker of type I collagen synthesis but, to date, no assay has been developed to measure PICP in guinea pig tissue extracts. A monoclonal antibody, known to cross-react with intact guinea pig procollagen type I (anti-PICP), was tested for its ability to bind soluble guinea pig PICP in crude skin extracts using a biosensor. Anti-PICP was immobilised to the surface of a sensor chip and antibody-antigen binding was detected using the phenomenon of surface plasmon resonance (SPR). The binding component in the SPR-immunoassay was identified as PICP by purification and N-terminal sequencing. Guinea pig PICP was purified from skin by gel filtration, ion exchange chromatography and lectin affinity chromatography. Purified PICP was then biotinylated and used with anti-PICP to develop a competition ELISA that was able to selectively and sensitively measure PICP in extracts of guinea pig connective tissue.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 297, Issues 1â2, February 2005, Pages 133-141
Journal: Journal of Immunological Methods - Volume 297, Issues 1â2, February 2005, Pages 133-141
نویسندگان
Helen L. Quasnichka, John F. Tarlton, Janet M. Anderson-MacKenzie, Michael E.J. Billingham, Allen J. Bailey, Andrew R. Pickford,