کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902379 | 1545802 | 2005 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of HLA-DRB1-bound self-peptides following measles virus infection
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کلمات کلیدی
MVVSelf-peptidesMOI - MEEBV - اپشتین بار ویروسSpectrum analysis - تجزیه و تحلیل طیفMass spectrometry - طیف سنجی جرمیMatrix-assisted laser desorption ionization time-of-flight mass spectrometry - طیف سنجی جرمی یونیزاسیون یونیزاسیون لیزر جذب ماتریسMALDI-TOF-MS - مالدی TOF-MSEpstein-Barr virus - ویروس Epstein-BarrMeasles virus - ویروس سرخکmultiplicity of infection - چندین عفونت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of HLA-DRB1-bound self-peptides following measles virus infection Identification of HLA-DRB1-bound self-peptides following measles virus infection](/preview/png/9902379.png)
چکیده انگلیسی
We developed a B-lymphocyte cell line derived from a measles seropositive individual who was homozygous for the HLA-DRB1*0301 allele. Peptides associated with the HLA-DRB1*0301 protein were purified from this lymphoblastoid cell line after infection with the Attenuvax⢠measles vaccine virus (Merck Research Laboratories, West Point, PA) and with “sham” infection. More than 40 peptide sequences were obtained by nano-scale reversed phase-high performance liquid chromatography coupled to tandem mass spectrometry (nano-LC/MS/MS). These peptides originated from 21 different source proteins-the majority from membrane-bound proteins. Most of the peptides (>73%) bound to HLA-DRB1*0301 appeared to be in lower abundance on measles-infected cells compared to the “sham-infected” cells. However, 26% of the identified peptides seem to have increased expression after measles infection. Measles vaccine virus infection did not change the level of HLA-DR expression. We demonstrate the power of nano-LC/MS/MS in the rapid determination of changes in the spectrum and expression of HLA-DRB1*0301-bound peptides after infection with measles virus. This provides further knowledge of the changes in peptide expression after viral infection and provides a powerful tool for identifying HLA-presented host and viral epitopes in the context of class II HLA molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 297, Issues 1â2, February 2005, Pages 153-167
Journal: Journal of Immunological Methods - Volume 297, Issues 1â2, February 2005, Pages 153-167
نویسندگان
Inna G. Ovsyannikova, Kenneth L. Johnson, Stephen Naylor, Gregory A. Poland,