Flow-cytometric screening of platelet antibodies with previously frozen cells

Platelets can be frozen using ThromboSol as the cryoprotectant, with controlled rate freezing and storage at −80 °C for the screening of platelet antibodies and for flow-cytometric crossmatch procedures. This system yields a reproducible and logistically simple method for platelet crossmatching that yields results superior to fresh cells and can be easily incorporated into standard clinical laboratory practices.