کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9905090 | 1546514 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Using a combination of cytochrome P450 1B1 and β-catenin for early diagnosis and prevention of colorectal cancer
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کلمات کلیدی
Bcl-2 expressionAHRp53ColorectumCYP1B1Adenomas - آدنوموسImmunohistochemistry - ایمونوهیستوشیمیbeta-catenin - بتا کاتینینDiagnosis - تشخیصEarly diagnosis - تشخیص زودهنگامDifferentiation - تفکیکDysplasia - دیسپلازی Colorectal cancer - سرطان روده بزرگCytochrome P450 - سیتوکروم پی۴۵۰Lymph node metastasis - متاستاز غدد لنفاویPrevention - پیشگیریaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background: Although fecal occult blood test and invasive endoscopic examination are common used to detect colorectal adenomas and cancers, non-invasive and specific biomarkers are still under investigation. The objective is to evaluate the biomarker CYP1B1 alone or in combination with aryl hydrocarbon receptor (AhR), nuclear β-catenin, p53 or bcl-2 for early diagnosis and prevention of colorectal cancer. Methods: These biomarkers were analyzed semi-quantified across 231 colonic tissues including 97 adenocarcinomas, 85 adenomas and 49 non-neoplastic colons using immunohistochemistry. In order to differentiate non-neoplastic colons from colorectal neoplasms (adenoma and carcinoma), the values for CYP1B1, AhR, nuclear β-catenin, p53 and bcl-2 expressions were subjected to discrimination analysis, then the cross-validation, sensitivity and specificity of these models were calculated. Results: Expressions of CYP1B1, p53, nuclear β-catenin and bcl-2 were significantly associated with colorectal carcinogenesis (p < 0.01 for the trend test). The overexpression rates for CYP1B1, p53, nuclear β-catenin and bcl-2 were significantly higher in the adenoma and carcinoma groups than in the non-neoplastic colon group (p < 0.05). The discrimination models showed that a combination of two biomarkers was better than a single biomarker, and provided specificity ranging from 39% to 100% and sensitivity ranging from 43% to 82% for colorectal carcinoma. Conclusions: The increase in expression of CYP1B1 occurred not only in colorectal carcinoma and but also in adenoma. Moreover, a screening panel of CYP1B1 in combination with nuclear β-catenin was the most suitable marker pair to screen for colorectal carcinoma based on this study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Detection and Prevention - Volume 29, Issue 6, 2005, Pages 562-569
Journal: Cancer Detection and Prevention - Volume 29, Issue 6, 2005, Pages 562-569
نویسندگان
Han MD, Jang-Ming MD, Chee C. MD, Lan C. BS, Chung H. DDS, MPH, Ming-Chih MD, PhD, Pinpin PhD,