کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9913319 | 1550308 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anti-tumor properties of human-activated macrophages produced in large scale for clinical application
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کلمات کلیدی
ADCCHSAIFN-γmAK - MAKhuman serum albumin - آلبومین سرم انسانیimmunotherapy - ایمونوتراپیtumor necrosis factor-alpha - تومور نکروز عامل آلفاCryopreservation - سرماداری Antibody-dependent cell cytotoxicity - سمیت سلولی وابسته به آنتی بادیTNF-α - فاکتور نکروز توموری آلفاAnti-tumor activity - فعالیت ضد تومورMacrophages - ماکروفاژها،درشت خوارهاNitric oxide - نیتریک اکسیدAntibody - پادتَن یا آنتیبادی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
When properly activated, macrophages can be tumoricidal. To harness the therapeutic potential of these cells, we have developed a process for ex vivo production of large numbers of IFN-γ-activated monocyte-derived macrophages. These monocyte-derived activated killer (MAK®) cells have been safely administered to cancer patients with minimal residual disease in phase I/II clinical studies. To evaluate efficacy of treatment with MAK cells, phase III clinical studies are necessary. The process of MAK cell production has been further optimized and qualified for use in large cohorts of patients. In this study, we characterized MAK cells produced in large scale by studying their phenotype and functions. MAK cells were shown to exert anti-tumor activity by killing tumor cells and inhibiting their proliferation. These activities were enhanced by activation with IFN-γ and addition of anti-tumor antibodies. Tumor necrosis factor-alpha (TNF-α) was one of the mediators used by MAK cells to inhibit tumor proliferation. To facilitate logistics of clinical trials, a process for MAK cell cryopreservation has been developed. We verified in vitro that cryopreserved cells retained the activity of fresh cells and were stable during storage. The safety and efficacy of cryopreserved MAK cells (Bexidem®) are currently being assessed on superficial bladder cancer patients in a phase II/III clinical trial.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 210, Issues 2â4, 19 August 2005, Pages 267-277
Journal: Immunobiology - Volume 210, Issues 2â4, 19 August 2005, Pages 267-277
نویسندگان
Véronique Baron-Bodo, Paula Doceur, Marie-Laure Lefebvre, Karine Labroquère, Catherine Defaye, Christophe Cambouris, Didier Prigent, Margarita Salcedo, Aurélie Boyer, Alessandra Nardin,