کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9954227 | 1542013 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and expression analysis of the critical floral gene MdAGL24-like in red-fleshed apple
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کلمات کلیدی
AP1AGL24SEP3SHORT VEGETATIVE PHASEAFLTFLGSTGFPTransgenic Arabidopsis - Arabidopsis ترانس ژنیکMurashige and Skoog - Murashige و SkoogHIS - خودDAY - روزlong days - روزهای طولانیanti - ضدTranscription factor - عامل رونویسیSVP - لطفاhistidine - هیستیدینAntibody - پادتَن یا آنتیبادیgreen fluorescent protein - پروتئین فلورسنت سبزMADS-box gene - ژن جعبه MADSFlowering - گل زدنglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The transition from vegetative to reproductive growth is the most dramatic phase change in plants. To better understand the molecular regulation of floral transition and flower development in red-fleshed apple (Malus sieversii f. niedzwetzkyana), we isolated and characterized a floral MADS-box gene, MdAGL24-like, which shares sequence similarity with AGAMOUS-LIKE 24 (AGL24) from other species. Spatial expression analysis showed that MdAGL24-like dynamically expressed in flowers, followed by roots and fruits. Subcellular localization analysis indicated that, like other transcript factors, MdAGL24-like was localized in the nucleus. Protein interaction analysis showed that MdAGL24-like could interact with MdSOC1 and MdAP1 in vivo and in vitro. MdAGL24-like and MdSOC1 could increase each other's expression by binding the CArG motifs in their promoters. Unlike MdSOC1, MdAGL24-like might indirectly promote the expression of MdLFY by upregulating the expression of MdSOC1. Ectopic expression of MdAGL24-like in wild-type Arabidopsis induced early flowering like the phenotypes induced by other AGL24 genes. Similar to AGL24 in Arabidopsis, MdAGL24-like could rescue the late-flowering phenotype of the agl24 mutant to some extent. These results help clarify the molecular mechanism underlying flowering and provide a means of shortening the juvenile period in red-fleshed apples and other fruit trees.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 276, November 2018, Pages 189-198
Journal: Plant Science - Volume 276, November 2018, Pages 189-198
نویسندگان
Mengyu Su, Nan Wang, Shenghui Jiang, Hongcheng Fang, Haifeng Xu, Yicheng Wang, Zhen Zhang, Jing Zhang, Lin Xu, Zongying Zhang, Xuesen Chen,