کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9955354 | 1570138 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and molecular characterization of a virulent systemic feline calicivirus isolated in China
ترجمه فارسی عنوان
جداسازی و شناسایی مولکولی یک کالیکویروس گربه سیستمیک ویروسی جدا شده در چین
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
چکیده انگلیسی
In this study, a virulent systemic (VS) feline calicivirus (FCV) strain, SH, was isolated from a household cat with severe systemic clinical signs, and its full-length genome was determined and analyzed. Through immunofluorescence assays (IFA) and western blotting assays, we found that FCV SH strain, like other isolates, can stably proliferate in Crandell feline kidney (CRFK) cells. Moreover, the typical morphology of FCV particles, with a diameter of about 35â¯nm, was observed using electron microscopy. The full-length genome of FCV strain SH was sequenced and determined to be 7704 nucleotides (nt) in length with a 5â²-terminal untranslated region (UTR) of 19â¯nt and a 3â²-terminal UTR of 67â¯nt. Three open reading frames (ORF1, ORF2, and ORF 3) were found within the genome, coding for a polypeptide, a capsid precursor (VP1) and a minor structural protein (VP2), respectively. Amino acid sequence comparison revealed diversity (from 82.2% to 88.5% homology) between the VP1 protein sequences of the SH/14 isolate and those of 33 reference isolates from different regions. Phylogenetic analyses using alignments of VP1 protein sequences showed that the SH/14 isolate shares the highest sequence homology with the reported VS-FCV George strain (88.5%), and is located in the same clade as other reported VS-FCV isolates, indicating that the FCV SH/14 strain is a VS-FCV isolate. However, the SH/14 strain does not belong to the same lineage as most other Chinese FCV isolates, suggesting that, in China, a very large geographical entity, the virulent systemic FCV might has emerged.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 65, November 2018, Pages 425-429
Journal: Infection, Genetics and Evolution - Volume 65, November 2018, Pages 425-429
نویسندگان
Huimin Guo, Qiuhong Miao, Jie Zhu, Zhibiao Yang, Guangqing Liu,