دانلود مقالات ISI درباره تکامل هدایت + ترجمه فارسی

Recent advances in discovery, heterologous expression, and molecular engineering of cyclodextrin glycosyltransferase for versatile applications

Keywords: تکامل هدایت; Cyclodextrin glycosyltransferase (CGTase); Heterologous expression; Molecular modification; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2014 سفارش ترجمه

Creating a TALE protein with unbiased 5′-T binding

Keywords: تکامل هدایت; Transcriptional activator-like effector (TALE); DNA binding; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2013 سفارش ترجمه

Enhancement of desulfurization activity by enzymes of the Rhodococcus dsz operon through coexpression of a high sulfur peptide and directed evolution

Keywords: تکامل هدایت; DBT; dibenzothiophene; dszABC; desulfurization operon genes A, B and C; S1; gene encoding the first Sulpeptide gene synthesized and cloned; S1; Sulpeptide 1; DCW; dry cell weight; SOE-PCR; sequence overlap extension polymerase chain reaction; Biodesulfuri

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Improving catalytic efficiency of endo-β-1, 4-xylanase from Geobacillus stearothermophilus by directed evolution and H179 saturation mutagenesis

Keywords: تکامل هدایت; Xylanase; Directed evolution; Error-prone PCR; Site-saturation mutagenesis; Catalytic efficiency;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

AXM mutagenesis: An efficient means for the production of libraries for directed evolution of proteins

Keywords: تکامل هدایت; Phage display; Affinity maturation; Directed evolution; Recombinant antibodies;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

From bricolage to BioBricks™: Synthetic biology and rational design

Keywords: تکامل هدایت; Synthetic biology; Rational design; Evolutionary electronics; Directed evolution; Ethics;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Microbial acid-stable α-amylases: Characteristics, genetic engineering and applications

Keywords: تکامل هدایت; Starch saccharification; α-Amylase; Acid-stable; Site directed mutagenesis; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2013 سفارش ترجمه

Enhanced acylation activity of esterase BioH from Escherichia coli by directed evolution towards improved hydrolysis activity

Keywords: تکامل هدایت; Enzyme technology; Biocatalysis; Enantioseparation; Enzyme activity; Escherichia coli BioH; Directed evolution

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2013 سفارش ترجمه

Directed evolution of Candida antarctica lipase B for kinetic resolution of profen esters

Keywords: تکامل هدایت; Biocatalysis; Directed evolution; Lipases; Kinetic resolution; Profens

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2013 سفارش ترجمه

Employing directed evolution for the functional analysis of multi-specific proteins

Keywords: تکامل هدایت; Multi-specific proteins; Directed evolution; Protein-networks

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases

Keywords: تکامل هدایت; Saccharomyces cerevisiae; Glucose oxidase; Directed evolution; High throughput screening; Chimera

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

In vitro selection of proteins via emulsion compartments

Keywords: تکامل هدایت; Directed evolution; In vitro compartmentalization; Emulsion; Genotype–phenotype linkage; In vitro transcription and translation; Droplet

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Directed evolution study unveiling key sequence factors that affect translation efficiency in Escherichia coli

Keywords: تکامل هدایت; Codon usage; Directed evolution; Green fluorescence protein; mRNA folding; Synonymous mutations; Translation efficiency

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Improving the thermostability of lipase Lip2 from Yarrowia lipolytica

Keywords: تکامل هدایت; Protein engineering; Directed evolution; Semi-rational design; B-FIT; Thermostability

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Hydroxylation of non-substituted polycyclic aromatic hydrocarbons by cytochrome P450 BM3 engineered by directed evolution

Keywords: تکامل هدایت; Cytochrome P450 BM3; Polycyclic aromatic hydrocarbons; Chrysene; Pyrene; Directed evolution

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Improved activity and thermostability of Bacillus pumilus lipase by directed evolution

Keywords: تکامل هدایت; Bacillus pumilus lipase; Biocatalyst; Directed evolution; DNA shuffling; Thermostability; 3D homology model

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Improvement of thermostable aldehyde dehydrogenase by directed evolution for application in Synthetic Cascade Biomanufacturing

Keywords: تکامل هدایت; Biocatalysis; Directed evolution; Enzyme engineering; Thermostable dehydrogenase; Screening; Synthetic Cascade BiomanufacturingAlDH, aldehyde dehydrogenase; TaAlDH, aldehyde dehydrogenase from Thermoplasma acidophilum

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol

Keywords: تکامل هدایت; ADH, alcohol dehydrogenase; NADH, nicotinamide adenine dinucleotide; Ll, Lactococcus lactisAlcohol dehydrogenase; Crystal structure; Site-saturation mutagenesis; Directed evolution; Isobutyraldehyde; Biofuel

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg

Keywords: تکامل هدایت; Directed evolution; Enzyme promiscuity; Perhydrolysis; Protein engineering; Subtilisin Carlsberg

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Generating thermal stable variants of protein domains through phage display

Keywords: تکامل هدایت; Affinity selection; Directed evolution; Fluorescence-based thermal shift assay; Phosphopeptide binding domain; Peptide ligands; Heat denaturation; Mutagenic polymerase chain reaction; SYPRO orange

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Directed evolution as a powerful synthetic biology tool

Keywords: تکامل هدایت; Synthetic biology; Directed evolution

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Janus: Prediction and Ranking of Mutations Required for Functional Interconversion of Enzymes

Keywords: تکامل هدایت; MSA, multiple sequence alignment; PDB, Protein Data Bank; PP, phenylpyruvate; DHDH, d-hydroxyisocaproate dehydrogenase; MDH, malate dehydrogenase; GDH, glutamate dehydrogenase; PEG, polyethylene glycolenzymology; protein engineering; directed evolution; c

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

Improvement of a puromycin-linker to extend the selection target varieties in cDNA display method

Keywords: تکامل هدایت; In vitro selection; mRNA/cDNA display; Puromycin; Directed evolution; Protein engineering

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2012 سفارش ترجمه

Development of an organo- and enzyme-catalysed one-pot, sequential three-component reaction

Keywords: تکامل هدایت; Aldolase; Three-component reaction; Organocatalysis; Directed evolution; Biocatalysis;

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2012 سفارش ترجمه

N-Acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possess N-acetyl lysine specificity in vitro and in vivo

Keywords: تکامل هدایت; N-Acetyl lysyl-tRNA synthetase; Directed evolution; CcdB

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes

Keywords: تکامل هدایت; Alkane; Biosensor; Directed Evolution; alkS;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Engineering a thermostable β-1,3-1,4-glucanase from Paecilomyces thermophila to improve catalytic efficiency at acidic pH

Keywords: تکامل هدایت; β-1,3-1,4-Glucanase; Directed evolution; Optimal pH; Site-directed mutagenesis; Paecilomyces thermophila

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Metabolism of chlorobiphenyls by a variant biphenyl dioxygenase exhibiting enhanced activity toward dibenzofuran

Keywords: تکامل هدایت; BPDO; biphenyl dioxygenase; GC-MS; gas chromatography-mass spectrometry; CB; chlorobiphenyl; PCB; polychlorinated biphenyl; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Polychlorinated biphenyls; Biphenyl dioxygenase; Enzym

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Enhancing the enzymatic activity of the endochitinase by the directed evolution and its enzymatic property evaluation

Keywords: تکامل هدایت; Directed evolution; Error-prone PCR; Endochitinase; Enzymatic property;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Easy preparation of a large-size random gene mutagenesis library in Escherichia coli

Keywords: تکامل هدایت; Directed evolution; Cherry fluorescent protein; High transformation efficiency; Escherichia coli; Prolonged overlap extension PCR; Simple cloning;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Directed evolution of proteins for increased stability and expression using yeast display

Keywords: تکامل هدایت; Yeast surface display; Directed evolution; Stability engineering; Antibody engineering; Library screening;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

DIRECTED EVOLUTION: SELECTION OF THE HOST ORGANISM

Keywords: تکامل هدایت; directed evolution; host organism; protein engineering; microalgae

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

Synthetic biology: New strategies for directing design

Keywords: تکامل هدایت; Genome engineering; Directed evolution; Synthetic biology; Recombineering

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

APPROACHES TO ENGINEER STABILITY OF BEETLE LUCIFERASES

Keywords: تکامل هدایت; Firefly luciferase; Bioluminescence; protein engineering; directed evolution; thermal stability

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

Temperature resistant mutants of Rhodobacter capsulatus generated by a directed evolution approach and effects of temperature resistance on hydrogen production

Keywords: تکامل هدایت; Rhodobacter capsulatus; Temperature resistance; EMS mutagenesis; Directed evolution; Hydrogen production; Photoheterotrophic growth

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

dRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method

Keywords: تکامل هدایت; Directed evolution; Diversity generation; SeSaM; epPCR; Ribavirin

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2012 سفارش ترجمه

Engineering antibodies by yeast display

Keywords: تکامل هدایت; Antibody engineering; Yeast display; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2012 سفارش ترجمه

Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast

Keywords: تکامل هدایت; IgG1; immunoglobulin G class 1; Fcab; antigen binding Fc protein; Fc-wt; recombinant wild-type Fc protein; scTCR; single-chain T-cell receptor; MHC; major histocompatibility complex; CDC; complement dependent cytotoxicity; ADCC; antibody dependent cell-me

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2012 سفارش ترجمه

Multiple amino acid substitutions significantly improve the thermostability of feruloyl esterase A from Aspergillus niger

Keywords: تکامل هدایت; Feruloyl esterase; Aspergillus niger; Thermostability; Pichia pastoris; Directed evolution

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2012 سفارش ترجمه

A novel genetic selection system for PLP-dependent threonine aldolases

Keywords: تکامل هدایت; Genetic complementation; Pyridoxal-5′-phosphate; Aldolase; Combinatorial mutagenesis; Catalysis; Mechanism; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

Targeted mutagenesis of the Vibrio fischeri flavin reductase FRase I to improve activation of the anticancer prodrug CB1954

Keywords: تکامل هدایت; CB1954; Nitroreductase; SOS response; Enzyme prodrug therapy; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

Site saturation mutagenesis: Methods and applications in protein engineering

Keywords: تکامل هدایت; SDM, site-directed mutagenesis; SSM, site-saturation mutagenesis; PCR, polymerase chain reaction; epPCR, error-prone PCRMutagenesis; Directed evolution; Structure–function; Codon-degeneracy; Biocatalysis; Protein engineering

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli

Keywords: تکامل هدایت; Directed evolution; Protein engineering; Error-prone PCR; Kunkel mutagenesis; Megaprimers;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

Biocatalyst development by directed evolution

Keywords: تکامل هدایت; Directed evolution; Biocatalysis; Enzyme engineering; High-throughput screening

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

Ribosome-independent biosynthesis of biologically active peptides: Application of synthetic biology to generate structural diversity

Keywords: تکامل هدایت; Combinatorial biosynthesis; Synthetic biology; Protein engineering; Metabolic engineering; Directed evolution; Ribosome-independent peptides;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2012 سفارش ترجمه

A molecular transporter engineering approach to improving xylose catabolism in Saccharomyces cerevisiae

Keywords: تکامل هدایت; Sugar transport; Directed evolution; Yeast; Pentose metabolism; Lignocellulose conversion;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2012 سفارش ترجمه

Theoretical consideration of selective enrichment in in vitro selection: Optimal concentration of target molecules

Keywords: تکامل هدایت; SELEX; Biopanning; In vitro evolution; Selection pressure; Evolutionary design; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2012 سفارش ترجمه

COMPUTER-AIDED PROTEIN DIRECTED EVOLUTION: A REVIEW OF WEB SERVERS, DATABASES AND OTHER COMPUTATIONAL TOOLS FOR PROTEIN ENGINEERING

Keywords: تکامل هدایت; directed evolution; rational design; semi-rational design; diversity generation; focused library; mutational effect

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2012 سفارش ترجمه

DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools

Keywords: تکامل هدایت; AVD; chicken avidin; AVR; avidin related protein; BBP-A; biotin-binding protein A; BTN; biotin; A/A2-1; chimeric protein containing sequence from avidin and AVR2 clone number 1; A/B-1; chimeric protein containing sequence from AVD and BBP-A clone number 1

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2012 سفارش ترجمه

Laboratory evolution of stereoselective enzymes as a means to expand the toolbox of organic chemists

Keywords: تکامل هدایت; Stereoselectivity; Enzymes; Directed evolution; Saturation mutagenesis; High-throughput screening;

دانلود رایگان متن کامل مقاله ISI 19 صفحه سال انتشار : 2012 سفارش ترجمه