دانلود مقالات ISI درباره تکامل هدایت + ترجمه فارسی

Directed evolution of the thermostable xylanase from Thermomyces lanuginosus

Keywords: تکامل هدایت; Directed evolution; Random mutagenesis; Thermomyces lanuginosus; Thermostability; Xylanase

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2007 سفارش ترجمه

Extending the diversity of cytochrome P450 enzymes by DNA family shuffling

Keywords: تکامل هدایت; CYP2C; Heterologous expression; Directed evolution; Molecular breeding; Indigo; Indole metabolismδ-ALA, δ-aminolevulinic acid; hNPR, human NADPH-cytochrome P450 reductase; IPTG, isopropyl β-d-thiogalactopyranoside; NADPH, reduced β-nicotinamide adenine di

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Photocleavable linkage between genotype and phenotype for rapid and efficient recovery of nucleic acids encoding affinity-selected proteins

Keywords: تکامل هدایت; Directed evolution; Drug receptor; Protein engineering; Proteomics; Puromycin

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Hybrid penicillin acylases with improved properties for synthesis of β-lactam antibiotics

Keywords: تکامل هدایت; Penicillin acylase; Ampicillin; Cephalexin; Synthesis; Biocatalysis; Gene shuffling; Directed evolution

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica

Keywords: تکامل هدایت; Directed evolution; Expression system; High-throughput screening; Protein engineering; Yarrowia lipolytica

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Global transcription machinery engineering: A new approach for improving cellular phenotype

Keywords: تکامل هدایت; Sigma factor engineering; Ethanol tolerance; Metabolic engineering; Directed evolution; Lycopene production

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

A novel screening assay for hydroxynitrile lyases suitable for high-throughput screening

Keywords: تکامل هدایت; HbHnl, Hevea brasiliensis hydroxynitrile lyase; MeHnl, Manihot esculenta hydroxynitrile lyase; PaHnl, Prunus amygdalus hydroxynitrile lyase; IPTG, isopropyl β-d-thiogalactopyranoside; DMF, dimethyl formamideHigh-throughput screening; Directed evolution; B

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2007 سفارش ترجمه

Extending the capabilities of nature's most versatile catalysts: Directed evolution of mammalian xenobiotic-metabolizing P450s

Keywords: تکامل هدایت; Cytochrome P450; Directed evolution; Biocatalysis; DNA shuffling; Indole;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2007 سفارش ترجمه

Directed evolution of enantioselective hybrid catalysts: a novel concept in asymmetric catalysis

Keywords: تکامل هدایت; Asymmetric catalysis; Enzymes; Hydrogenation; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2007 سفارش ترجمه

Random strand transfer recombination (RSTR) for homology-independent nucleic acid recombination

Keywords: تکامل هدایت; Homologous recombination; Non-homologous recombination; Protein engineering; Promoter engineering; Strand transfer; Template switch; Directed evolution

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2007 سفارش ترجمه

Stability and activity improvement of cephalosporin esterase EstB from Burkholderia gladioli by directed evolution and structural interpretation of muteins

Keywords: تکامل هدایت; Esterase; Burkholderia gladioli; Cephalosporin C; Directed evolution; Stability; Activity

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2007 سفارش ترجمه

A shuffled CYP2C library with a high degree of structural integrity and functional versatility

Keywords: تکامل هدایت; Cytochrome P450; CYP2C; DNA shuffling; Biocatalysts; Directed evolution

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2007 سفارش ترجمه

Inverting enantioselectivity of Burkholderia gladioli esterase EstB by directed and designed evolution

Keywords: تکامل هدایت; Directed evolution; Enantioselectivity; Esterase; High-throughput screening

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2007 سفارش ترجمه

Directed evolution for drug and nucleic acid delivery

Keywords: تکامل هدایت; Molecular evolution; Directed evolution; Antibody affinity maturation; Viral gene therapy; ADEPT; GDEPT;

دانلود رایگان متن کامل مقاله ISI 17 صفحه سال انتشار : 2007 سفارش ترجمه

A colorimetric assay for screening transketolase activity

Keywords: تکامل هدایت; Colorimetric assay; Transketolase; Directed evolution; Tetrazolium red

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2006 سفارش ترجمه

Making glucose oxidase fit for biofuel cell applications by directed protein evolution

Keywords: تکامل هدایت; Biofuel cell; Glucose oxidase; Directed evolution; High-throughput screening; GODA

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Improving protein solubility: The use of the Escherichia coli dihydrofolate reductase gene as a fusion reporter

Keywords: تکامل هدایت; Directed evolution; Protein solubility; Gene cassette; Fusion protein; Dihydrofolate reductase

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Improved thermostability and the optimum temperature of Rhizopus arrhizus lipase by directed evolution

Keywords: تکامل هدایت; Rhizopus arrhizus; Lipase; Stability; Directed evolution; DNA shuffling; Error-prone PCR

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2006 سفارش ترجمه

Improved solubility of TEV protease by directed evolution

Keywords: تکامل هدایت; TEV protease; Directed evolution; GFP; Solubility; FACS

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2006 سفارش ترجمه

In vitro evolution of proteins

Keywords: تکامل هدایت; directed evolution; mutagenesis; protein sequence space

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2006 سفارش ترجمه

Femtoliter compartment in liposomes for in vitro selection of proteins

Keywords: تکامل هدایت; Liposomes; Fluorescence-activated cell sorter; In vitro translation; Directed evolution

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2006 سفارش ترجمه

Directed evolution of plant basic helix–loop–helix transcription factors for the improvement of transactivational properties

Keywords: تکامل هدایت; Plant transcription factors; Basic helix–loop–helix transcription activators; Transactivation; Acidic domain; Interaction domain; Directed evolution

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2006 سفارش ترجمه

Directed evolution for improved secretion of cancer–testis antigen NY-ESO-1 from yeast

Keywords: تکامل هدایت; Directed evolution; Tumor antigen; NY-ESO-1; Yeast surface display; Fluorescence activated cell sorting; High-throughput screening

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2006 سفارش ترجمه

Identifying the interacting positions of a protein using Boolean learning and support vector machines

Keywords: تکامل هدایت; Interacting position; OCAT; Directed evolution; Support vector machines; Boolean function; Recombinations;

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2006 سفارش ترجمه

Evolution on distributive lattices

Keywords: تکامل هدایت; Fitness landscape; Distributive lattice; Directed evolution; Risk polynomial; Chain polynomial; HIV drug resistance; Bayesian network; Mutagenetic tree

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2006 سفارش ترجمه

Novel biocatalysts: Recent developments

Keywords: تکامل هدایت; Biocatalysis; Biochemical engineering; Catalyst selectivity; Directed evolution; Metabolic screening

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2006 سفارش ترجمه

A Statistical Analysis of Random Mutagenesis Methods Used for Directed Protein Evolution

Keywords: تکامل هدایت; protein engineering; directed evolution; random mutagenesis; bias indicator; MAPepPCR, error-prone PCR; MAP, mutagenesis assistant program

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2006 سفارش ترجمه

Directed Evolution of DNA Polymerase, RNA Polymerase and Reverse Transcriptase Activity in a Single Polypeptide

Keywords: تکامل هدایت; spCSR, short-patch compartmentalized self-replication; wt, wild-type; ELISA, enzyme-linked immunosorbent assayDNA polymerases; directed evolution; protein engineering; PCR; enzyme evolution

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2006 سفارش ترجمه

Engineering of Kex2 variants exhibiting altered substrate specificity

Keywords: تکامل هدایت; Kex2; Protease; Engineering; Substrate specificity; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2005 سفارش ترجمه

Mathematical expressions useful in the construction, description and evaluation of protein libraries

Keywords: تکامل هدایت; Protein engineering; Directed evolution; Random mutagenesis; Cassette mutagenesis; Genetic libraries;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2005 سفارش ترجمه

Parallel capillary electrophoresis for the quantitative screening of fermentation broths containing natural products

Keywords: تکامل هدایت; Parallel capillary electrophoresis; Aspergillus terreus; Strain improvement; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2005 سفارش ترجمه

In vivo directed evolution for thermostabilization of Escherichia coli hygromycin B phosphotransferase and the use of the gene as a selection marker in the host-vector system of Thermus thermophilus

Keywords: تکامل هدایت; Thermus thermophilus; directed evolution; thermostabilization; selection marker; hygromycin resistance; HPH; hygromycin B phosphotransferase; HTK; a highly thermostable kanamycin nucleotidyltransferase; Hyg; hygromycin B; Kan; kanamycin;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2005 سفارش ترجمه

High-throughput assays for lipases and esterases

Keywords: تکامل هدایت; High-throughput screening; Assay; Lipase; Esterase; Hydrolase; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2005 سفارش ترجمه

Directed evolution for engineering pH profile of endoglucanase III from Trichoderma reesei

Keywords: تکامل هدایت; Trichoderma reesei; Endoglucanase III; Directed evolution; Site-directed mutagenesis; Asparagine-321;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2005 سفارش ترجمه

Alteration of substrate specificity of aspartase by directed evolution

Keywords: تکامل هدایت; Aspartase; Directed evolution; Alteration of substrate specificity;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2005 سفارش ترجمه

Whole plasmid mutagenic PCR for directed protein evolution

Keywords: تکامل هدایت; Beta-galactosidase; Random mutagenesis; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2005 سفارش ترجمه

Increased expression of a bacterial phosphotriesterase in Escherichia coli through directed evolution

Keywords: تکامل هدایت; Phosphotriesterase; Directed evolution; Organophosphate; Pathway engineering;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2005 سفارش ترجمه

Strategies and computational tools for improving randomized protein libraries

Keywords: تکامل هدایت; Directed evolution; Library; Molecular diversity; Random mutagenesis; Redundancy;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2005 سفارش ترجمه

Engineering the substrate specificity of cytochrome P450 CYP102A2 by directed evolution: production of an efficient enzyme for bioconversion of fine chemicals

Keywords: تکامل هدایت; BMP; the heme domain of P450 BM3; CPR; NADPH: cytochrome P450 reductase; CYP; cytochrome P450; CYP102 or P450 BM3; cytochrome P450 monooxygenase from Bacillus megaterium; CYP102A2; cytochrome P450 monooxygenase from Bacillus subtilis; FAD; flavin adenine

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2005 سفارش ترجمه

Directed evolution of (βα)8-barrel enzymes

Keywords: تکامل هدایت; (βα)8-Barrel; TIM-barrel; Directed evolution; Random mutagenesis;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2005 سفارش ترجمه

Directed evolution of biocatalytic processes

Keywords: تکامل هدایت; Directed evolution; Biocatalysis; Process; Metabolic engineering; Recombineering; Microscale;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2005 سفارش ترجمه

Directed evolution: selecting today's biocatalysts

Keywords: تکامل هدایت; HTS; high throughput screening; epPCR; error-prone polymerase chain reaction; NRR; non-homologous random recombination; SeSaM; sequence saturation mutagenesis; ADO; assembly of designed oligonucleotides; DHR; degenerate homoduplex gene family recombinatio

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2005 سفارش ترجمه

Use of directed evolution of mammalian cytochromes P450 for investigating the molecular basis of enzyme function and generating novel biocatalysts

Keywords: تکامل هدایت; Cytochrome P450; P450; Directed evolution; Random mutagenesis; Biocatalysts;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2005 سفارش ترجمه

Generation of a strong promoter for Escherichia coli from eukaryotic genome DNA

Keywords: تکامل هدایت; Promoter; Regulation; Expression; Error prone PCR; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2005 سفارش ترجمه

Recent advances in the bioremediation of persistent organic pollutants via biomolecular engineering

Keywords: تکامل هدایت; Persistent organic pollutants; Biomolecular engineering; Bioremediation; Directed evolution; Bph; biphenyl dioxygenase; CB; chlorobiphenyls; CBA; (chloro)benzoate; CYP; cytochrome P450 monooxygenase; GEM; genetically engineered microorganism; IVC; in vitr

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2005 سفارش ترجمه

Directed evolution of novel polymerases

Keywords: تکامل هدایت; Polymerase; DNA; RNA; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2005 سفارش ترجمه

Support vector machines for learning to identify the critical positions of a protein

Keywords: تکامل هدایت; Support vector machines; Identifying critical positions; Machine learning; Amino-acid sequence; β-Lactamase; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2005 سفارش ترجمه

An expanding genetic code

Keywords: تکامل هدایت; Unnatural amino acid incorporation; Orthogonal tRNA-synthetase pair; Protein engineering; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2005 سفارش ترجمه

Directed molecular evolution by machine learning and the influence of nonlinear interactions

Keywords: تکامل هدایت; Directed evolution; Genetic algorithm; DNA shuffling; NK landscape; Machine learning;

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2005 سفارش ترجمه