دانلود مقالات ISI درباره تکامل هدایت + ترجمه فارسی

Library-based methods for identification of soluble expression constructs

Keywords: تکامل هدایت; Protein expression; Random library; Solubility; Automation; Directed evolution; Protein engineering;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2011 سفارش ترجمه

A high-throughput screen for directed evolution of aminocoumarin amide synthetases

Keywords: تکامل هدایت; Directed evolution; Aminocoumarin; High-throughput screening; Natural products

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2011 سفارش ترجمه

Fungal laccases: Versatile tools for lignocellulose transformation

Keywords: تکامل هدایت; High redox potential laccases; Lignin; Directed evolution; Industrial application

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2011 سفارش ترجمه

Challenges in the directed evolution of stereoselective enzymes for use in organic chemistry

Keywords: تکامل هدایت; Asymmetric; Catalysis; Biocatalysis; Directed evolution; Enantioselectivity; EnzymesCatalyse asymétrique; Biocatalyse; Évolution dirigée; Énantioselectivité; Enzyme

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2011 سفارش ترجمه

Directed evolution of a thermophilic endoglucanase (Cel5A) into highly active Cel5A variants with an expanded temperature profile

Keywords: تکامل هدایت; Thermoanaerobacter tengcongensis; Directed evolution; Error-prone PCR; Endoglucanase; Molecular Dynamics

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2011 سفارش ترجمه

Engineering of cellobiose phosphorylase for glycoside synthesis

Keywords: تکامل هدایت; Cellobiose phosphorylase; Enzyme engineering; Glycoside synthesis; Directed evolution

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2011 سفارش ترجمه

One-step production of lactate from cellulose as the sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis

Keywords: تکامل هدایت; Bacillus subtilis; Cellulase engineering; Consolidated bioprocessing; Endoglucanase; Lactate; Metabolic engineering; Directed evolution

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2011 سفارش ترجمه

Enzymatic and whole cell catalysis: Finding new strategies for old processes

Keywords: تکامل هدایت; Biocatalysis; Biotransformation; Bioconversion; Directed evolution; Bacterial adaptation; Kinetic resolution

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2011 سفارش ترجمه

ORF-selector ESPRIT: A second generation library screen for soluble protein expression employing precise open reading frame selection

Keywords: تکامل هدایت; High-throughput screening; Directed evolution; Soluble protein; Incremental truncation library; IKK1; Protein purification; p85α; Random library;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2011 سفارش ترجمه

Chemical profiling of the deacetylase activity of acetyl xylan esterase A (AxeA) variants on chitooligosaccharides using hydrophilic interaction chromatography–mass spectrometry

Keywords: تکامل هدایت; AxeA, acetyl xylan esterase A; AxeAtr, truncated acetyl xylan esterase A; HILIC, hydrophilic interaction chromatography; CE, capillary electrophoresis; A6, hexa-N-acetyl-glucosamineBiocatalysis; Protein engineering; Directed evolution; Random mutagenesis;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2011 سفارش ترجمه

Directed evolution of a thermostable l-aminoacylase biocatalyst

Keywords: تکامل هدایت; Biocatalysis; l-Aminoacylase; Directed evolution; Saturation mutagenesis; Amino acids

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2011 سفارش ترجمه

Laboratory evolution of an epoxide hydrolase - Towards an enantioconvergent biocatalyst

Keywords: تکامل هدایت; Directed evolution; Regioselectivity; Enantioconvergence; Styrene oxide; Enantioselectivity; Deracemization;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2011 سفارش ترجمه

Elucidating acetate tolerance in E. coli using a genome-wide approach

Keywords: تکامل هدایت; Acetate; Directed evolution; Cellulosic feedstock; Genome library; Selection

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2011 سفارش ترجمه

Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution

Keywords: تکامل هدایت; computational protein design; directed evolution; enzymatic catalysisTS, transition state; PDB, Protein Data Bank; MD, molecular dynamics; Bistris, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol; PEG, polyethylene glycol; ESRF, European Sy

دانلود رایگان متن کامل مقاله ISI 22 صفحه سال انتشار : 2011 سفارش ترجمه

Effects of catalytic site mutations on active expression of phage fused penicillin acylase

Keywords: تکامل هدایت; Penicillin G acylase; Intein; Phage display; Directed evolution; Mutagenesis; Post-translational auto-splicing;

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2010 سفارش ترجمه

Directed evolution of tyrosinase for enhanced monophenolase/diphenolase activity ratio

Keywords: تکامل هدایت; Tyrosinase; Directed evolution; Selectivity; Catechol

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2010 سفارش ترجمه

Laboratory evolution of laccase for substrate specificity

Keywords: تکامل هدایت; Directed evolution; Laccase; Substrate specificity; Thermal stability; Protein engineering

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2010 سفارش ترجمه

Single-step bioconversion for the preparation of l-gulose and l-galactose

Keywords: تکامل هدایت; l-Gulose; l-Galactose; Directed evolution; Phosphotransferase system; Mannitol-1-dehydrogenase

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2010 سفارش ترجمه

Molecular evolution of Theta-class glutathione transferase for enhanced activity with the anticancer drug 1,3-bis-(2-chloroethyl)-1-nitrosourea and other alkylating agents

Keywords: تکامل هدایت; Glutathione transferase (GST); 1,3-Bis-(2-chloroethyl)-1-nitrosourea (BCNU); Chloroethylnitrosoureas (CENUs); Directed evolution; Alkyltransferase; Alkylating agents;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2010 سفارش ترجمه

Improving the thermostability of Geobacillus stearothermophilus xylanase XT6 by directed evolution and site-directed mutagenesis

Keywords: تکامل هدایت; Xylanase; Protein engineering; Directed evolution; Thermostability; Semi-rational

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2010 سفارش ترجمه

Directed evolution of Alcaligenes faecalis nitrilase

Keywords: تکامل هدایت; Enzyme catalysis; Nitrilase; Directed evolution; Mutation; pH stability

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2010 سفارش ترجمه

Directed evolution of angiotensin II-inhibiting peptides using a microbead display

Keywords: تکامل هدایت; Single-molecule PCR; Microbead display; Cell-free protein synthesis; Directed evolution; Angiotensin II

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2010 سفارش ترجمه

Enhanced secretory production of hemolysin-mediated cyclodextrin glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system

Keywords: تکامل هدایت; Alpha-hemolysin transport system; Cyclodextrin glucanotransferase; Directed evolution; Extracellular secretion; Escherichia coli

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2010 سفارش ترجمه

Sites in the AAV5 capsid tolerant to deletions and tandem duplications

Keywords: تکامل هدایت; Directed evolution; AAV; Viral gene delivery;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2010 سفارش ترجمه

New approaches to enzymatic glycoside synthesis through directed evolution

Keywords: تکامل هدایت; Directed evolution; Screening; Glycosynthases; Glycosides; Glycosphingolipids

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2010 سفارش ترجمه

Quantitative Y2H screening: Cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter

Keywords: تکامل هدایت; Quantitative screening; Y2H; Fungal beta-galactosidase; LacA; Directed evolution

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2010 سفارش ترجمه

Optimization of stealth adeno-associated virus vectors by randomization of immunogenic epitopes

Keywords: تکامل هدایت; Adeno-associated virus; Directed evolution; Capsid library; Capsid engineering; Pre-existing immunity; Antibody neutralization; Antibody escaping; Capsid biology; Vector optimization; Tropism

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2010 سفارش ترجمه

Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase

Keywords: تکامل هدایت; Thermostable lactonase; Nerve agent hydrolysis; Directed evolution; Enzyme activation

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2010 سفارش ترجمه

Evolved Lactococcus lactis Strains for Enhanced Expression of Recombinant Membrane Proteins

Keywords: تکامل هدایت; GFP, green fluorescent protein; KPi, potassium phosphateevolved expression hosts; membrane proteins; directed evolution; GFP; folding indicator

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2010 سفارش ترجمه

Structure-function relationships of epoxide hydrolases and their potential use in biocatalysis

Keywords: تکامل هدایت; Epoxide hydrolase; Structure-activity relationship; Mechanism; Stereospecificity; Biocatalysis; New enzymes; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2010 سفارش ترجمه

Expression of an alkalo-tolerant fungal xylanase enhanced by directed evolution in Pichia pastoris and Escherichia coli

Keywords: تکامل هدایت; Xylanase; Directed evolution; Yeast expression; Pichia pastoris

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Improvement of alkaline lipase from Proteus vulgaris T6 by directed evolution

Keywords: تکامل هدایت; Proteus vulgaris lipase; Directed evolution; DNA shuffling; Error-prone PCR

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Development of a bacteriophage-based system for the selection of structured peptides

Keywords: تکامل هدایت; Structured peptides; Phage display; Protease resistance; Directed evolution; Tryptophan zipper

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2009 سفارش ترجمه

Efficient screening for new amino acid dehydrogenase activity: Directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid

Keywords: تکامل هدایت; Directed evolution; Biocatalysis; Phenylalanine dehydrogenase; Error-prone PCR; High-throughput screening; Parallel over-expression

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Heterologous expression, purification, and characterization of cytochrome P450sca-2 and mutants with improved solubility in Escherichia coli

Keywords: تکامل هدایت; Cytochrome P450sca-2; Recombinant expression; E. coli; Solubility; Directed evolution; GFP

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Structural features of T cell receptor variable regions that enhance domain stability and enable expression as single-chain VαVβ fragments

Keywords: تکامل هدایت; T cell receptor; Single-chain; Yeast display; Directed evolution

دانلود رایگان متن کامل مقاله ISI 15 صفحه سال انتشار : 2009 سفارش ترجمه

Examination and expansion of the substrate range of m-hydroxybenzoate hydroxylase

Keywords: تکامل هدایت; Comamonas testosteroni; m-Hydroxybenzoate hydroxylase; Directed evolution; Substrate specificity; Phenol; 3-Aminophenol; Substituted catechol

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2008 سفارش ترجمه

Expression in yeast of secreted lignin peroxidase with improved 2,4-dichlorophenol degradability by DNA shuffling

Keywords: تکامل هدایت; Lignin peroxidase; DNA shuffling; Directed evolution; LiP expression in yeast; 2,4-DCP degradation activity; Stability against H2O2

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2008 سفارش ترجمه

Protein domain library generation by overlap extension (PDLGO): A tool for enzyme engineering

Keywords: تکامل هدایت; Directed evolution; Random mutagenesis; Error-prone PCR; EstE; Esterase; Whole-cell biocatalyst

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2008 سفارش ترجمه

Functionality improvement of fungal lignin peroxidase by DNA shuffling for 2,4-dichlorophenol degradability and H2O2 stability

Keywords: تکامل هدایت; Liginin peroxidase; Directed evolution; DNA shuffling; 2,4-DCP degradability; H2O2 stability; Yeast surface display

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2008 سفارش ترجمه

A biosensor for all d-amino acids using evolved d-amino acid oxidase

Keywords: تکامل هدایت; RgDAAO; d-Amino acids; Biosensor; Food quality; Directed evolution; Flavoprotein

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2008 سفارش ترجمه

Implication of a mutation in the flavin binding site on the specific activity and substrate specificity of glycine oxidase from Bacillus subtilis produced by directed evolution

Keywords: تکامل هدایت; GOX, glycine oxidase; d-AAO, d-amino acid oxidase; SOX, sarcosine oxidase; HT, high-troughputGlycine oxidase; Directed evolution; High-throughput screening; Pipecolic acid; Homology modelling

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2008 سفارش ترجمه

Directed evolution of a maltogenic α-amylase from Bacillus sp. TS-25

Keywords: تکامل هدایت; Directed evolution; Bread staling; Thermal stability; Maltooligosaccharides; Retrogradation; Starch amylopectin

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2008 سفارش ترجمه

Saturation mutagenesis reveals the importance of residues αR145 and αF146 of penicillin acylase in the synthesis of β-lactam antibiotics

Keywords: تکامل هدایت; Penicillin acylase; Antibiotic synthesis; Ampicillin; Directed evolution; Protein engineering; Mutagenesis

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2008 سفارش ترجمه

Directed evolution of aldolases for exploitation in synthetic organic chemistry

Keywords: تکامل هدایت; Directed evolution; Aldolases; Substrate specificity; Stereochemistry; Organic synthesis; Carbon-carbon bond formation;

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2008 سفارش ترجمه

Investigating protein structural plasticity by surveying the consequence of an amino acid deletion from TEM-1 β-lactamase

Keywords: تکامل هدایت; Δ; amino acid deleted; Amp; ampicillin; Cam; chloramphenicol; CAZ; ceftazidime; Indel; insertion-deletion; MIC; minimum inhibitory concentration; Protein plasticity; Directed evolution; Deletion mutations; Indels; Antibiotic resistance;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2007 سفارش ترجمه

Dual selection of a genetic switch by a single selection marker

Keywords: تکامل هدایت; Genetic circuit; Genetic switch; Directed evolution; Genetic selection

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2007 سفارش ترجمه

High-throughput Screening of the Enantioselectivity of Hyperthermophilic Mutant Esterases from Archaeon Aeropyrum pernix K1 for Resolution of (R,S) -2-Octanol Acetate

Keywords: تکامل هدایت; High-throughput screening; Enantioselectivity; Hyperthermophilic esterase; Directed evolution;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2007 سفارش ترجمه

Nascent chain, mRNA, and ribosome complexes generated by a pure translation system

Keywords: تکامل هدایت; In vitro translation; Ribosome display; Directed evolution; PURE system;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2007 سفارش ترجمه

A set of UV-inducible autolytic vectors for high throughput screening

Keywords: تکامل هدایت; Directed evolution; High throughput screening; UV induction; Autolysis; SRRz lysis cassette

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2007 سفارش ترجمه