کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
17585 | 42681 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Directed evolution of Alcaligenes faecalis nitrilase
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Alcaligenes faecalis nitrilase (NITAf) was engineered in a directed evolution approach towards increased activity at its optimal pH as well as improved fitness at low pH values. Error prone PCR in combination with recombination of beneficial mutations resulted in a variant with increased specific activity for 2-phenylpropionitrile at pH 7.5. In addition, a new nitrilase variant (pHNIT45) was developed that is catalytically active at pH values as low as pH 4.5. Within 10 min this mutant fully hydrolyzes the base labile substrate (R)-2-Cl-mandelonitrile (10 mM) to give the product (R)-2-Cl-mandelic acid (conversion 100%, ee > 99%) with full retention of enantiopurity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 47, Issue 4, 6 September 2010, Pages 140–146
Journal: Enzyme and Microbial Technology - Volume 47, Issue 4, 6 September 2010, Pages 140–146
نویسندگان
Ulrike Schreiner, Bianca Hecher, Sascha Obrowsky, Kerstin Waich, Norbert Klempier, Georg Steinkellner, Karl Gruber, J. David Rozzell, Anton Glieder, Margit Winkler,