Michaelis-Menten equation for degradation of insoluble substrate
Keywords: سینتیک آنزیم; Enzyme kinetics; Michaelis-Menten; Parameter estimation;
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Spectrophotometric determination of reaction rates and kinetic parameters of a BAHD acyltransferase using DTNB (5,5â²-dithio-bis-[2-nitrobenzoic acid])
Keywords: سینتیک آنزیم; CoA; coenzyme A; DOPA; 3,4-dihydroxyphenylalanine; DTNB; 5,5â²-dithio-bis-(2-nitrobenzoic acid); HCT; hydroxycinnamoyl-coenzyme A hydroxycinnamoyl transferase; HDT; hydroxycinnamoyl-CoA: l-3,4-dihydroxyphenylalanine hydroxycinnamoyl transferase; LC; liqu
Molecular and biochemical characterization of natural and recombinant phosphoglycerate kinase B from Trypanosoma rangeli
Keywords: سینتیک آنزیم; Trypanosoma rangeli; Phosphoglycerate kinase; Glycolysis; Carbohydrate metabolism regulation; Isoenzymes; Enzyme kinetics; 1,3BPGA; 1,3-bisphosphoglycerate; 3PGA; 3-phosphoglycerate; PGK; phosphoglycerate kinase; rPGK; recombinant PGK; TrPGK; PGK from T.Â
Zebrafish bio-assay guided isolation of human acetylcholinesterase inhibitory trans-tephrostachin from Tephrosia purpurea (L.) Pers
Keywords: سینتیک آنزیم; trans-Tephrostachin; Zebrafish; Enzyme kinetics; Acetylcholinesterase; Molecular docking and dynamics;
Analyzing the function of the insert region found between the α and β-subunits in the eukaryotic nitrile hydratase from Monosiga brevicollis
Keywords: سینتیک آنزیم; Nitrile hydratase; Enzyme kinetics; Cobalt; Protein biosynthesis; Metal transport; And metal insertion; NHase; nitrile hydratase; ORF; open reading frame; ICP-MS; inductively-coupled plasma mass spectrometry; IMAC; immobilized metal affinity chromatograph
Application of ultra high-performance liquid chromatography tandem mass spectrometry to investigate the regioselective glucuronidation of icaritin in vitro
Keywords: سینتیک آنزیم; Enzyme kinetics; Glucuronidation/Diglucuronidation; UDP-glucuronosyltransferase/UGT; Regioselectivity;
From electrochemistry to enzyme kinetics of cytochrome P450
Keywords: سینتیک آنزیم; CYP17A1; cytochrome P450 17A1; DDAB; didodecyldimethylammonium bromide; KPi; potassium phosphate buffer; SPE; screen-printed electrode; CV; cyclic voltammetry; Cytochrome b5mc; microsomal cytochrome b5; Cytochrome b5om; mitochondrial cytochrome b5; Cytoch
Enzymatic and spectroscopic properties of a thermostable [NiFe]âhydrogenase performing H2-driven NAD+-reduction in the presence of O2
Keywords: سینتیک آنزیم; Hydrogenase; Hydrogen; Oxyhydrogen reaction; Nickel; Iron; Respiratory Complex I; Flavin; Ironâsulfur cluster; Pyridine nucleotide; Enzyme kinetics; Infrared vibrational spectroscopy; Electron paramagnetic resonance spectroscopy; Nuclear resonance vibra
Characterization of regioselective flavonoid O-methyltransferase from the Streptomyces sp. KCTC 0041BP
Keywords: سینتیک آنزیم; O-Methyltransferase; Flavonoid; Region-selective methylation; Enzyme kinetics;
The kinetic analysis of the N-methylation of 4-phenylpyridine by nicotinamide N-methyltransferase: Evidence for a novel mechanism of substrate inhibition
Keywords: سینتیک آنزیم; 4-PP; 4-phenylpyridine; DMSO; dimethylsulphoxide; DV; dependent variable; hNNMT; recombinant human NNMT; IV; independent variable; kcat/Km; catalytic efficiency; MeNH; 2N-methylnorharman; MMP; mitochondrial membrane potential; MPP+; 1-methyl-4-phenylpyrid
White shrimp Litopenaeus vannamei recombinant lactate dehydrogenase: Biochemical and kinetic characterization
Keywords: سینتیک آنزیم; LDH; Biochemical and kinetic characterization; Shrimp; Enzyme kinetics; Homotetramer;
Functional characterization of a soluble NADPH-cytochrome P450 reductase from Fusarium graminearum
Keywords: سینتیک آنزیم; Cytochrome c; Thiazolyl blue tetrazolium bromide; Enzyme kinetics; Trichothecenes; Sterol biosynthesis; CYP; Cytochrome P450; CPR; NADPH-cytochrome P450 reductase; FgCPR; Fusarium graminearum NADPH-cytochrome P450 reductase with N-terminal truncation (Î1
An eco-friendly approach to control Oxya velox infestation: Mangifera indica exoglucanase and endoglucanase cellulose ingestion inhibition activity
Keywords: سینتیک آنزیم; Enzyme kinetics; Oxya velox; Cellulase inhibitors; Exoglucanase; Endoglucanase;
The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro
Keywords: سینتیک آنزیم; PARK8; ROCO4; Dardarin; Enzyme kinetics; Manganese; Nucleotide-binding pocket;
Short communicationsPre-clinical evidence for the efficacy and safety of α-amylase inhibitory peptides from cumin (Cuminum cyminum) seed
Keywords: سینتیک آنزیم; α-Amylase inhibition; Bioactive peptides; Cumin seed; Cell-based assay; Enzyme kinetics;
Mechanistic insights into EgGST1, a Mu class glutathione S-transferase from the cestode parasite Echinococcus granulosus
Keywords: سینتیک آنزیم; Echinococcus granulosus; Glutathione transferase; Enzyme kinetics; Fluorescence spectroscopy; Glutathione; EgGST1; glutathione transferase-1 from E. granulosus; rEgGST1; recombinant EgGST1; GSH; glutathione; CDNB; 1-chloro-2,4-dinitrobenzene; MES; 2-(N-m
Substitutions in the cardenolide binding site and interaction of subunits affect kinetics besides cardenolide sensitivity of insect Na,K-ATPase
Keywords: سینتیک آنزیم; Na,K-ATPase; Amino acid substitutions; Subunit interaction; Enzyme kinetics; Cardenolide resistance; Drosophila;
In vitro inhibitory mechanisms and molecular docking of 1â²-S-1â²-acetoxychavicol acetate on human cytochrome P450 enzymes
Keywords: سینتیک آنزیم; 1â²-S-1â²-acetoxychavicol acetate; Drug interaction; Cytochrome P450; Inhibition; Enzyme kinetics; Molecular docking; ACA; 1â²-s-1â²-acetoxychavicol acetate; BOMCC; 7-benzyloxymethyloxy-3-cyanocoumarin; CYPS; cytochrome p450s; DBOMF; dibenzyloxymethyl
Probing the activity of NTHL1 orthologs by targeting conserved amino acid residues
Keywords: سینتیک آنزیم; DHT; 5,6-dihydrothymine; DHU; 5,6-dihydrouracil; DTT; dithiothreitol; Tg; 5,6-dihydro-5,6-dihydroxythymine (thymine glycol); hNTHL1; human endonuclease III; EcoNth; E. coli endonuclease III; Endonuclease III; Base excision repair; Enzyme kinetics;
Functional analysis of TcCTLP-5C2, a chymotrypsin-like serine protease needed for molting in Tribolium castaneum
Keywords: سینتیک آنزیم; Chymotrypsin; Cuticle; Enzyme kinetics; N-glycosylation; Midgut; Molting fluid; Red flour beetle; serine proteinases; Tribolium castaneum;
Analysis of plant UDP-arabinopyranose mutase (UAM): Role of divalent metals and structure prediction
Keywords: سینتیک آنزیم; Plant mutase; Reversibly glycosylated polypeptides; UDP-arabinopyranose mutase; L-arabinofuranose; Structure prediction; Enzyme kinetics; Metalloproteins;
Biochemical and kinetic evaluation of the enzymatic toxins from two stinging scyphozoans Nemopilema nomurai and Cyanea nozakii
Keywords: سینتیک آنزیم; Jellyfish venom; Enzymatic toxins; Enzyme kinetics; Metalloproteinase; Nemopilema nomurai; Cyanea nozakii;
Research articleEnzymatic characterization and crystal structure analysis of Chlamydomonas reinhardtii dehydroascorbate reductase and their implications for oxidative stress
Keywords: سینتیک آنزیم; Chlamydomonas; Dehydroascorbate reductase; X-ray crystallography; Enzyme kinetics; Site-directed mutagenesis; Oxidative stress;
Rate-based Modelling and Validation of a Pilot Absorber Using MDEA Enhanced with Carbonic Anhydrase (CA)
Keywords: سینتیک آنزیم; CO2; GM enhancement factor; Enzyme kinetics; Multiple reversible reactions; Model validation;
The BRENDA enzyme information system-From a database to an expert system
Keywords: سینتیک آنزیم; Enzyme database; Metabolic pathways; Enzyme-catalysed reactions; Enzyme kinetics; Enzymes and diseases; Enzyme-ligand interaction;
Synthesis, screening and docking of fused pyrano[3,2-d]pyrimidine derivatives as xanthine oxidase inhibitor
Keywords: سینتیک آنزیم; Pyrano[3,2-d]pyrimidine; Xanthine oxidase enzyme; Enzyme kinetics; Lineweaver-Burk plot; Molecular modeling studies;
Penicillin acylase and O-aryloxycarbonyl hydroxamates: Two acyl-enzymes, one leading to hydrolysis, the other to inactivation
Keywords: سینتیک آنزیم; Penicillin acylase; N-terminal hydrolase; O-Aryloxycarbonyl hydroxamates; Enzyme kinetics; Enzyme inactivation; Enzyme mechanism;
Kinetic resolution of (R,S) phenyl glycidyl ether by red mung beans (Vigna angularis) epoxide hydrolases
Keywords: سینتیک آنزیم; Kinetic resolution; (R,S)-Phenyl glycidyl ether; Red Mung beans; Epoxy Hydrolase; Enzyme kinetics;
Comparison of ALS functionality and plant growth in ALS-inhibitor susceptible and resistant Myosoton aquaticum L.
Keywords: سینتیک آنزیم; Acetolactate synthase (ALS) mutation; Enzyme kinetics; Herbicide resistance; Myosoton aquaticum L.; RGR;
Characterization of AKR1B16, a novel mouse aldo-keto reductase
Keywords: سینتیک آنزیم; Aldose reductase inhibitor; Enzyme kinetics; Gene orthology; Retinaldehyde reductase; Steroid oxidoreductase; Subcellular localization; Î4-Ae; androst-4-ene-3,17-dione; AKR; aldo-keto reductase; AKR1B16; AKR family member 1B16; ARI; aldose reductase inhi
NMR studies of the non-haem Fe(II) and 2-oxoglutarate-dependent oxygenases
Keywords: سینتیک آنزیم; 2OG; 2-Oxoglutarate; BBOX; γ-Butyrobetaine hydroxylase; CODD; C-terminal oxygen-dependent degradation domain; COSY; Correlation spectroscopy; DSBH; Double-stranded β-helix; EFE; Ethylene-forming enzyme; FIH; Factor-inhibiting hypoxia-inducible factor; H
Intrinsic protein fluorescence assays for GEF, GAP and post-translational modifications of small GTPases
Keywords: سینتیک آنزیم; GTPases; GEFs; GAPs; AnkX; Enzyme kinetics; Intrinsic protein fluorescence; DrrA/SidM; defect in Rab recruitment A, substrate of ICM/Dot transporter M; AnkX; Ankyrin repeat-containing protein X Phosphocholine transferase; Lem3; phosphocholine hydrolase; S
A facile method to prepare large quantities of active caspase-3 overexpressed by auto-induction in the C41(DE3) strain
Keywords: سینتیک آنزیم; Caspase-3; Auto-induction; C41(DE3); Soluble expression; Enzyme kinetics;
Kinetic resolution of (R,S)-α-tetralol catalyzed by crosslinked Candida antarctica lipase B enzyme supported on mesocellular foam: A nanoscale enzyme reactor approach
Keywords: سینتیک آنزیم; C. antarctica lipase B; Mesoporous microcellular foam (MCF); Enantio-selectivity; α-tetralol; Enzyme kinetics
Expression, purification, enzymatic characterization and crystallization of glyceraldehyde-3-phosphate dehydrogenase from Naegleria gruberi, the first one from phylum Percolozoa
Keywords: سینتیک آنزیم; Naegleria gruberi; Glyceraldehyde-3-phosphate dehydrogenase; Enzyme kinetics; Crystallization; NgGAPDH; glyceraldehyde-3-phosphate dehydrogenase from Naegleria gruberi;
Selective production of 1-monocaprin by porcine liver carboxylesterase-catalyzed esterification: Its enzyme kinetics and catalytic performance
Keywords: سینتیک آنزیم; Porcine liver carboxylesterase; Esterification; Reverse micelles; 1-monocaprin; Enzyme kinetics
A highly active bile salt hydrolase from Enterococcus faecalis shows positive cooperative kinetics
Keywords: سینتیک آنزیم; Bile salt hydrolase; Gut organism; Bile acids; Allosteric regulation; Penicillin V acylase; Enzyme kinetics;
Homodimerization of UDP-glucuronosyltransferase 2B7 (UGT2B7) and identification of a putative dimerization domain by protein homology modeling
Keywords: سینتیک آنزیم; UDP-glucuronosyltransferase; UGT2B7; Homology model; Dimerization; Enzyme kinetics; Glucuronidation
Purification and characterization of aspartate N-acetyltransferase: A critical enzyme in brain metabolism
Keywords: سینتیک آنزیم; Canavan disease; aspartate N-acetyltransferase; membrane protein; enzyme kinetics; enzyme purification;
Substrate quality affects kinetics and catalytic efficiency of exo-enzymes in rhizosphere and detritusphere
Keywords: سینتیک آنزیم; Enzyme kinetics; Enzyme affinity; Performance constant; Substrate-induced respiration; Microbial biomass;
The human tyrosine kinase Kit and its gatekeeper mutant T670I, show different kinetic properties: Implications for drug design
Keywords: سینتیک آنزیم; Kit; Enzyme kinetics; Tyrosine kinases; Drug resistance;
Platform for determining the inhibition profile of neuraminidase inhibitors in an influenza virus N1 background
Keywords: سینتیک آنزیم; Antiviral; Susceptibility; Resistance; Enzyme kinetics; Fitness; Drug design;
Purification and properties of glycerol-3-phosphate dehydrogenase from the liver of the hibernating ground squirrel, Urocitellus richardsonii
Keywords: سینتیک آنزیم; Enzyme kinetics; Hibernation; Metabolic rate depression; Reversible protein phosphorylation; Post-translational modification; Urocitellus richardsonii
Ketoconazole Stereoisomers Differentially Induce Cytochrome P450 1A1 Between Human Hepatoma HepG2 and Mouse Hepatoma Hepa1c1c7 Cells
Keywords: سینتیک آنزیم; cytochrome P450; dose-response; drug metabolizing enzymes; enzyme kinetics; enzymes; hepatic clearance; hepatic metabolism; kinetics;
Two variants of the major serine protease inhibitor from the sea anemone Stichodactyla helianthus, expressed in Pichia pastoris
Keywords: سینتیک آنزیم; Heterologous expression; Pichia pastoris; BPTI-Kunitz; Serine protease; Enzyme kinetics; Tight-binding inhibition; Site-directed mutagenesis; Sea anemone3D, three-dimensional; BAPA (Bz-Arg-pNA), N-benzoyl-arginine-p-nitroanilide; BPTI, bovine pancreatic t
DDPH, a novel antihypertensive agent, is a potential dual inhibitor of hepatic CYP2D and CYP3A
Keywords: سینتیک آنزیم; DDPH; Cytochrome P450; Propafenone; Drug–drug interaction; Enzyme kinetics
In search of lost time constants and of non-Michaelis-Menten parameters
Keywords: سینتیک آنزیم; Enzyme kinetics; Michaelis-Menten equation; Mathematical model; Renewal theory; Non-Michaelis-Menten enzyme kinetics;
Caenorhabditis elegans AGXT-1 is a mitochondrial and temperature-adapted ortholog of peroxisomal human AGT1: New insights into between-species divergence in glyoxylate metabolism
Keywords: سینتیک آنزیم; Primary hyperoxaluria; Enzyme kinetics; Substrate specificity; Protein stability; Conformational disease
In vitro and in vivo inhibition of aldose reductase and advanced glycation end products by phloretin, epigallocatechin 3-gallate and [6]-gingerol
Keywords: سینتیک آنزیم; Diabetes; Aldose reductase; Enzyme kinetics; Bioactive compounds; Functional foods;
Assembly of ligands interaction models for glutathione-S-transferases from Plasmodium falciparum, human and mouse using enzyme kinetics and molecular docking
Keywords: سینتیک آنزیم; Enzyme kinetics; Molecular docking; PfGST; hGSTP1; mGSTM1