کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5132007 1378787 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro
چکیده انگلیسی


- The LRRK2 kinase domain can use GTP for both autophosphorylation and trans peptide phosphorylation activity.
- The pathological mutation G2019S increased GTP-dependent kinase activity.
- LRRK2 utilization of GTP as a phospho-donor changes the preferences of LRRK2 autophosphorylation.

The evolutionarily conserved enzyme encoded by the leucine-rich repeat kinase 2 gene, LRRK2, harbors both a Rab-like GTPase domain and a serine/threonine protein kinase domain. Pathogenic mutations in either the GTPase or kinase domain can cause neurodegeneration and Parkinson disease. No high-resolution structure of the human LRRK2 kinase domain is available but the most common mutation, G2019S in the kinase domain, is predicted to alter the ATP-binding pocket structure and interaction with divalent cations. Here we find that the manganese-bound kinase domain acquires a robust ability to utilize both GTP as well as ATP in autophosphorylation of the GTPase domain and phosphorylation of peptide substrates in vitro. The G2019S LRRK2 mutation increases the efficiency of GTP-mediated kinase activity ten-fold compared to WT LRRK2 activity. Moreover, GTP-dependent phosphorylation alters autophosphorylation-site preference in vitro. While additional studies are required to determine the physiological relevance of these observations, LRRK2 is one of the only known kinases to be able to utilize GTP as a phospho-donor at physiological levels in vitro, and thus one of the only known proteins to be able to hydrolyze GTP in two distinct domains within the same protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1865, Issue 3, March 2017, Pages 274-280
نویسندگان
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