Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata
Keywords: کلونینگ ژن; Cristaria plicata; Selenoprotein W (SelW); Gene cloning; mRNA expression
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Production optimization and molecular structure characterization of a newly isolated novel laccase from Fusarium solani MAS2, an anthracene-degrading fungus
Keywords: کلونینگ ژن; Gene cloning; Fusarium solani; Identification; Laccase; Response surface methodology
Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda
Keywords: کلونینگ ژن; Exopalaemon carinicauda; Calreticulin (CRT); Gene cloning; Vibrio anguillarum; White spot syndrome virus (WSSV)
A type I pullulanase of Bacillus cereus Nws-bc5 screening from stinky tofu brine: Functional expression in Escherichia coli and Bacillus subtilis and enzyme characterization
Keywords: کلونینگ ژن; Stinky tofu brine; Strain screening; Pullulanase; Gene cloning; Enzymatic properties; Bacillus subtilis expression
Molecular cloning, bacterial expression and functional characterisation of cytochrome P450 monooxygenase, CYP97C27, and NADPH-cytochrome P450 reductase, CPR I, from Croton stellatopilosus Ohba
Keywords: کلونینگ ژن; CYP; Cytochrome P450 monooxygenases; CPR; Cytochrome P450 reductase; RACE; Rapid Amplification of cDNA Ends; ER; endoplasmic reticulum; RT-PCR; Reverse transcription polymerase chain reaction; SDS-PAGE; sodium dodecyl sulphate-polyacrylamide gel electroph
Isolation of gamma-interferon-inducible lysosomal thiol reductase (GILT) from the Yangtze finless porpoise
Keywords: کلونینگ ژن; Finless porpoise; GILT; Gene cloning; Protein purification; Biological functions analysis;
Biodegradation of phenol using recombinant plasmid-carrying Rhodococcus erythropolis strains
Keywords: کلونینگ ژن; Rhodococcus erythropolis; Phenol degradation; Wastewater; Gene cloning; Plasmid;
Molecular cloning and biochemical characterization of the first Na+-channel α-type toxin peptide (Acra4) from Androctonus crassicauda scorpion venom
Keywords: کلونینگ ژن; Alpha-toxin; Amino acid sequence; Androctonus crassicauda; Gene cloning; Scorpion;
Molecular structure, expression pattern and functional characterisation of APRIL in an aquatic mammal
Keywords: کلونینگ ژن; APRIL; A proliferation inducing ligand; TNF; tumour necrosis factor; fAPRIL; finless porpoise APRIL cDNA; RACE; the rapid amplification of cDNA ends; pET43.1a-fsAPRIL; recombinant soluble fAPRIL; DE3; Escherichia coli BL21; fsAPRIL; soluble fAPRIL; IUCN;
Expression profiles of selenium dependent glutathione peroxidase and glutathione S-transferase from Exopalaemon carinicauda in response to Vibrio anguillarum and WSSV challenge
Keywords: کلونینگ ژن; Exopalaemon carinicauda; Glutathione peroxidase (GPx); Glutathione S-transferases (GST); Gene cloning; Expression;
Identification of IL-34 in teleost fish: Differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor
Keywords: کلونینگ ژن; Teleost fish; IL-34; MCSF; MCSFR; Gene cloning; Differential expression;
Molecular cloning, characterization and immunological response analysis of Toll-like receptor 21 (TLR21) gene in grass carp, Ctenopharyngodon idella
Keywords: کلونینگ ژن; Grass carp; TLR21; Aquareovirus; Aeromonas hydrophila; Gene cloning; Characterization
Hereditary cataract of the Nakano mouse: Involvement of a hypomorphic mutation in the coproporphyrinogen oxidase gene
Keywords: کلونینگ ژن; cataract; mouse model; gene cloning; mutation; coproporphyrin
Gene cloning and function analysis of cytokine-induced suppressor of cytokine signaling (SOCS) from pearl oyster Pinctada fucata
Keywords: کلونینگ ژن; Pearl oyster; PfSOCS-2; Gene cloning; Immune response
Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization
Keywords: کلونینگ ژن; Pearl oyster; PfIL-17; Gene cloning; Immune response
Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice
Keywords: کلونینگ ژن; Oryza sativa; leafy head mutant; genetic analysis; gene cloning; P450 CYP78A11 gene;
Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata
Keywords: کلونینگ ژن; Pearl oyster; PfIRF-2; Gene cloning; Immune response
Gene identification and recombinant protein of a lysozyme from freshwater mussel Cristaria plicata
Keywords: کلونینگ ژن; Cristaria plicata; Lysozyme (LYZ); Gene cloning; Expression; Recombinant protein
Molecular characterization of lipoprotein lipase from blunt snout bream Megalobrama amblycephala and the regulation of its activity and expression by dietary lipid levels
Keywords: کلونینگ ژن; Blunt snout bream; Lipoprotein lipase; Gene cloning; Tissue expression; Dietary lipid
Identification, expression profiling of a grass carp TLR8 and its inhibition leading to the resistance to reovirus in CIK cells
Keywords: کلونینگ ژن; Grass carp (Ctenopharyngodon idella); CIK cells; TLR8; Gene cloning; Antiviral activity; Grass carp reovirus
Molecular cloning and characterization of sea bass (Dicentrarchus labrax, L.) MHC class I heavy chain and β2-microglobulin
Keywords: کلونینگ ژن; Dicentrarchus labrax; β2m; MHC class I; Gene cloning; Tissue-specific expression; Homology modeling
Isolation of laccase gene from Bacillus subtilis and analysis of its physicochemical properties
Keywords: کلونینگ ژن; CICC, China Center of Industrial Culture Collection; PDA, Potato Dextrose Agar; NCBI, National Center for Biotechnology Information; lac, gene of laccase; Lac, lac gene encoding proteinLaccase; Gene cloning; Expression; Enzyme activity; Physicochemical pr
Cloning and expression of a gene with phospholipase B activity from Pseudomonas fluorescens in Escherichia coli
Keywords: کلونینگ ژن; Phospholipase B; Gene cloning; Expression; Pseudomonasfluorescens; Escherichiacoli;
Purification, gene cloning and characterization of an acidic β-1,4-glucanase from Phialophora sp. G5 with potential applications in the brewing and feed industries
Keywords: کلونینگ ژن; Phialophora sp. G5; β-1,4-Glucanase; Purification; Gene cloning; Animal feed; Brewing
Gene cloning and recombinant expression of a novel fungal immunomodulatory protein from Trametes versicolor
Keywords: کلونینگ ژن; Trametes versicolor; Fungal immunomodulatory protein; Gene cloning; Protein expression; Cytokines;
Molecular cloning, tissue distribution, and ontogeny of gonadotropin-releasing hormone III gene (GnRH-III) in half-smooth tongue sole (Cynoglossus semilaevis)
Keywords: کلونینگ ژن; C. semilaevis, Cynoglossus semilaevis; csGnRH-III, Cynoglossus semilaevis GnRH-III; qPCR, quantitative real-time PCR; DPF, days post fertilizationCynoglossus semilaevis; GnRH-III; Gene cloning; Promoter analysis
Purification and cDNA cloning of a novel neurotoxic peptide (Acra3) from the scorpion Androctonus crassicauda
Keywords: کلونینگ ژن; A. crassicauda; Androctonus crassicauda; α-NaScTxs; alpha sodium channel toxins; β-NaScTxs; beta sodium channel toxins; HPLC; high performance liquid chromatography; Nav1.1-1.6; voltage-dependent sodium channels 1.1-1.6; TFA; trifluroacetic acid; TS
Purification and characterization of a novel thermostable α-l-arabinofuranosidase (α-l-AFase) from Chaetomium sp.
Keywords: کلونینگ ژن; CAPS; (cyclohexylamino)-1-propanesulphonic acid; CHES; 2-(cyclohexylamino)ethanesulfonic acid; GH; glycoside hydrolase; MES; 2-(N-morpholino)ethanesulfonic acid; MOPS; 3-(N-morpholino)-propanesulfonic acid; PDA; potato dextrose-agar; pNP; p-nitrophenyl; S
Molecular cloning and characterization of class I NF-κB transcription factor from pearl oyster (Pinctada fucata)
Keywords: کلونینگ ژن; Pearl oyster; Class I NFκB transcription factor; Gene cloning; Immunity response
Cloning, overexpression, purification and characterization of Plasmodium knowlesi lactate dehydrogenase
Keywords: کلونینگ ژن; Plasmodium knowlesi; Malaria; Gene cloning; Recombinant lactate dehydrogenase; Malaria diagnosis
Isolation of anthocyanin biosynthetic genes in red Chinese sand pear (Pyrus pyrifolia Nakai) and their expression as affected by organ/tissue, cultivar, bagging and fruit side
Keywords: کلونینگ ژن; Pyrus pyrifolia; Bagging; Anthocyanin accumulation; Gene cloning; Gene expression
Cloning, identification and functional characterization of a pi-class glutathione-S-transferase from the freshwater mussel Cristaria plicata
Keywords: کلونینگ ژن; Cristaria plicata; Glutathione-S-transferase (GST); Gene cloning; Expression; Protein characterization
Molecular cloning and characterization of sea bass (Dicentrarchus labrax, L.) Tapasin
Keywords: کلونینگ ژن; Dicentrarchus labrax; Tapasin; Gene cloning; Comparative sequence analysis; Protein structure prediction;
Rice functional genomics research: Progress and implications for crop genetic improvement
Keywords: کلونینگ ژن; Oryza sativa; Functional genomic resources; Gene cloning; Rice 2020; Green Super Rice
A protein phosphatase 1 gamma (PP1γ) of the human protozoan parasite Trichomonas vaginalis is involved in proliferation and cell attachment to the host cell
Keywords: کلونینگ ژن; Trichomonas vaginalis; Proliferation; Cell attachment; Protein phosphatase 1; Gene cloning; Expression and genomic organisation
Cloning of the IL-1β3 gene and IL-1β4 pseudogene in salmonids uncovers a second type of IL-1β gene in teleost fish
Keywords: کلونینگ ژن; Rainbow trout; Atlantic salmon; Type I IL-1β; Type II IL-1β; Gene cloning; Expression
Cloning and optimized expression of a neutral endoglucanase gene (ncel5A) from Volvariella volvacea WX32 in Pichia pastoris
Keywords: کلونینگ ژن; Volvariella volvacea; Gene cloning; Endoglucanase optimized expression; Enzymatic properties; Pichia pastoris
Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium thermophilum: Characterization using recombinant protein
Keywords: کلونینگ ژن; Symbiobacterium thermophilum; Family M42 aminopeptidase; Gene cloning; Recombinant protein; Enzyme characterization
Cloning and expression analysis of kenaf (Hibiscus cannabinus L.) major lignin and cellulose biosynthesis gene sequences and polymer quantification during plant development
Keywords: کلونینگ ژن; 4cl, 4-coumarate:CoA ligase; c4 h, cinnamate 4-hydroxylase; cad, cinnamyl alcohol dehydrogenase; ccr, cinnamoyl-CoA oxidoreductase; CeSA, cellulose synthaseKenaf (Hibiscus cannabinus L.); Lignin; Cellulose; Gene cloning; qPCR
Molecular cloning, characterization and expression analysis of interferon-β promoter stimulator 1 (IPS-1) gene from grass carp Ctenopharyngodon idella
Keywords: کلونینگ ژن; Grass carp (Ctenopharyngodon idella); IPS-1; Gene cloning; mRNA expression; Grass carp reovirus
Cloning and functional characterization of TCRP1, a novel gene mediating resistance to cisplatin in an oral squamous cell carcinoma cell line
Keywords: کلونینگ ژن; Gene cloning; TCRP1; OSCC; Cisplatin; Apoptosis;
Identification of a retinoic acid-inducible gene I from grass carp (Ctenopharyngodon idella) and expression analysis in vivo and in vitro
Keywords: کلونینگ ژن; Grass carp (Ctenopharyngodon idella); RIG-I; Gene cloning; mRNA expression; Grass carp reovirus
Invasiveness and anchorage independent growth ability augmented by PTEN inactivation through the PI3K/AKT/NFkB pathway in lung cancer cells
Keywords: کلونینگ ژن; PTEN; AKT; NFkB; Invasion; Lung cancer; Gene cloning;
Transporters associated with antigen processing (TAP) in sea bass (Dicentrarchus labrax, L.): Molecular cloning and characterization of TAP1 and TAP2
Keywords: کلونینگ ژن; Dicentrarchus labrax; TAP; Gene cloning; Comparative sequence analysis; Protein structure prediction; Homology modelling;
Biochemical and molecular characterization of the venom from the Cuban scorpion Rhopalurus junceus
Keywords: کلونینگ ژن; Cuba; Gene cloning; Ion-channel; Scorpion toxin; Rhopalurus junceus;
AiCTL-6, a novel C-type lectin from bay scallop Argopecten irradians with a long C-type lectin-like domain
Keywords: کلونینگ ژن; Argopecten irradians; C-type lectin; Innate immunity; Gene cloning; Real-time PCR; Recombinant expression; Bacterial agglutination
High-throughput protein purification and quality assessment for crystallization
Keywords: کلونینگ ژن; Domain design; Expression vectors; Gene cloning; Protein purification; Crystallization screening; Quality assessment;
Scorpion and spider venom peptides: Gene cloning and peptide expression
Keywords: کلونینگ ژن; EST; Expression; Gene cloning; Scorpion; Spider; Toxin;
An efficient and rapid method for gene cloning from eukaryotic genomic DNA using overlap-PCR: With an example of cattle Ghrelin gene
Keywords: کلونینگ ژن; Overlap-PCR; Megeprimer; Gene cloning; Genomic DNA; Gene splicing; Cattle Ghrelin gene
Cloning and overexpression of Pochonia chlamydosporia chitinase gene pcchi44, a potential virulence factor in infection against nematodes
Keywords: کلونینگ ژن; Pochonia chlamydosporia; Chitinase; Gene cloning; Expression; Phylogenetic analysis; Km value