کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
21051 43204 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium thermophilum: Characterization using recombinant protein
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium thermophilum: Characterization using recombinant protein
چکیده انگلیسی

The chromosomal DNA of the syntrophic thermophile Symbiobacterium thermophilum contains open reading frames of the genes encoding family M42 aminopeptidases, Pep1079, Pep1080, and Pep1081. To characterize these peptidases, the genes were cloned into Escherichia coli and overexpressed. Our experiments using the recombinant proteins confirmed that Pep1079, Pep1080, and Pep1081 are components of arginyl or lysinyl aminopeptidases that require Co2+ for enzymatic activity. Coexistence of Pep1079 and Pep1080 is necessary for expressing high peptidase activity. Pep1081 enhances the activity of Pep1079 and Pep1080.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 2, February 2011, Pages 134–139
نویسندگان
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