Working with OpusXpress: Methods for high volume oocyte experiments
Keywords: بیان هترولوژیک; Oocytes; High throughput; Ion channels; Heterologous expression; Drug development;
مقالات ISI بیان هترولوژیک (ترجمه نشده)
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Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli
Keywords: بیان هترولوژیک; Heterologous expression; Serine proteinase inhibitor; Sinapis alba L.; Trypsin inhibition; Escherichia coli
Growth arrest of Synechocystis sp. PCC6803 by superoxide generated from heterologously expressed Rhodobacter sphaeroides chlorophyllide a reductase
Keywords: بیان هترولوژیک; Chlorophyllide a reductase; Superoxide generation; Heterologous expression; Growth cessation; Rhodobacter sphaeroides; Synechocystis sp. PCC6803;
Crystallization NoteAcrB et al.: Obstinate contaminants in a picogram scale. One more bottleneck in the membrane protein structure pipeline
Keywords: بیان هترولوژیک; AcrB; Succinate dehydrogenase; Heterologous expression; Picogram crystallization; Membrane protein contaminants; Structural genomics;
Improved tryprostatin B production by heterologous gene expression in Aspergillus nidulans
Keywords: بیان هترولوژیک; Aspergillus; Secondary metabolism; NRPS; Tryprostatin; Fumitremorgin; Heterologous expression
Heterologous expression in Escherichia coli of Neurospora crassa neutral trehalase as an active enzyme
Keywords: بیان هترولوژیک; Neutral trehalase; Trehalose; Neurospora crassa; Filamentous fungi; Heterologous expression
Structure–function studies on the voltage-gated sodium channel
Keywords: بیان هترولوژیک; Voltage sensor; Activation-inactivation coupling; Heterologous expression; Electrophysiology; Secondary structure; Molecular modelling
Determination of the extent of phosphopantetheinylation of polyketide synthases expressed in Escherichia coli and Saccharomyces cerevisiae
Keywords: بیان هترولوژیک; Phosphopantetheinylation; Polyketide synthase; Heterologous expression; 6-Deoxyerythronolide B synthase; Lovastatin polyketide synthase;
Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris
Keywords: بیان هترولوژیک; Xylanase; Thermomyces lanuginosus; Protein purification; Physicochemical characterisation; Heterologous expression
Expression of HBsAg and preS2-S protein in different yeast based system: A comparative analysis
Keywords: بیان هترولوژیک; Heterologous expression; HBsAg; PreS2-S protein; Hepatitis B virus; Saccharomyces cerevisiae; Pichia pastoris;
Overexpression and purification of rat peroxisomal membrane protein 22, PMP22, in Pichia pastoris
Keywords: بیان هترولوژیک; PMP22; Pichia pastoris; Heterologous expression; Membrane protein; Fed-batch fermentation; Peroxisome
Alkoxyresorufin (methoxy-, ethoxy-, pentoxy- and benzyloxyresorufin) O-dealkylase activities by in vitro-expressed cytochrome P450 1A4 and 1A5 from common cormorant (Phalacrocorax carbo)
Keywords: بیان هترولوژیک; AROD; Common cormorant; P450; CYP1A4; CYP1A5; Heterologous expression; Yeast
Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain
Keywords: بیان هترولوژیک; Avian influenza virus; H5N1; Heterologous expression; Neuraminidase (NA); Pichia pastoris
Expression of soluble versatile peroxidase of Bjerkandera adusta in Escherichia coli
Keywords: بیان هترولوژیک; Versatile peroxidase; Bjerkandera adusta; Manganese peroxidase; Lignin peroxidase; Heterologous expression
Domain-specific determinants of catalysis/substrate binding and the oligomerization status of barley UDP-glucose pyrophosphorylase
Keywords: بیان هترولوژیک; aa, amino acids; AGX, UDP-N-acetylglucosamine pyrophosphorylase; NB loop, nucleotide binding; PPi, pyrophosphate; UDP-glucose, UDPG; UGPase, UDPG pyrophosphorylaseEnzyme kinetics; Heterologous expression; Oligomerization; Protein domains; Protein structur
Phenylalanine 35 and tyrosine 82 are involved in the uptake and release of ligand by porcine odorant-binding protein
Keywords: بیان هترولوژیک; Porcine odorant-binding protein; Molecular dynamics; Heterologous expression; Pichia pastoris; Fluorescence spectroscopy; 1-aminoanthracene
High titer production of tetracenomycins by heterologous expression of the pathway in a Streptomyces cinnamonensis industrial monensin producer strain
Keywords: بیان هترولوژیک; Heterologous expression; Tetracenomycin; Precursor supply; Polyketide; Monensin; Crotonyl-CoA reductase; StreptomycesCCR, crotonyl-CoA reductase; DEBS, 6-deoxyerythronolide B synthase; PKS, polyketide synthase; TCM, tetracenomycin
Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan
Keywords: بیان هترولوژیک; Thermotolerant yeast; Hansenula polymorpha; Heterologous expression; Amylolytic enzymes; Fermentation of starch; Xylan-degrading enzymes; Xylan fermentation
A complex of three related membrane proteins is conserved on malarial merozoites
Keywords: بیان هترولوژیک; Apicomplexa; Plasmodium; Merozoite; Transmembrane proteins; Heterologous expression;
Recombinant Mycobacterium bovis BCG
Keywords: بیان هترولوژیک; Mycobacterium bovis BCG; Recombinant BCG; Heterologous expression; Immunomodulator
Identification, recombinant expression, and biochemical characterization of a flavonol 3-O-glucosyltransferase clone from Citrus paradisi
Keywords: بیان هترولوژیک; Citrus paradisi; Rutaceae; Grapefruit; Enzymology; Flavonoid; Flavonol; Glucosyltransferase; Heterologous expression;
Pyranose 2-oxidase from Phanerochaete chrysosporium—Expression in E. coli and biochemical characterization
Keywords: بیان هترولوژیک; Pyranose 2-oxidase; Phanerochaete chrysosporium; Lignocellulose degradation; Heterologous expression
Structural model and functional characterization of the Bemisia tabaci CYP6CM1vQ, a cytochrome P450 associated with high levels of imidacloprid resistance
Keywords: بیان هترولوژیک; Bemisia tabaci; Metabolic resistance; Neonicotinoid insecticide; Imidacloprid; Cytochrome P450 CYP6CM1vQ; Heterologous expression; Molecular modeling
A review of experimental techniques used for the heterologous expression of nicotinic acetylcholine receptors
Keywords: بیان هترولوژیک; Nicotinic acetylcholine receptor; Heterologous expression
A reliable transformation method and heterologous expression of β-glucuronidase in Lentinula edodes
Keywords: بیان هترولوژیک; Electroporation; β-glucuronidase; Heterologous expression; Lentinula edodes
Expression, purification and immunodetection of a recombinant fragment (residues 179–281) of the G protein from rabies virus ERA strain
Keywords: بیان هترولوژیک; Rabies virus G protein; Heterologous expression; Rabies virus diagnostic method
Heterologous expression of functionally active enterolysin A, class III bacteriocin from Enterococcus faecalis, in Escherichia coli
Keywords: بیان هترولوژیک; Class III bacteriocin; Enterolysin A; Heterologous expression; Escherichia coli
Functional expression of Helicoverpa armigera CYP9A12 and CYP9A14 in Saccharomyces cerevisiae
Keywords: بیان هترولوژیک; Helicoverpa armigera (Hübner); CYP9A12; CYP9A14; Heterologous expression; Esfenvalerate
Bitter peptides activate hTAS2Rs, the human bitter receptors
Keywords: بیان هترولوژیک; Bitter taste; hTAS2Rs; Heterologous expression; Bitter peptide; Casein hydrolysate
Isotopic labeling of c-type multiheme cytochromes overexpressed in E. coli
Keywords: بیان هترولوژیک; Multiheme cytochromes; Escherichia coli; Isotopic labeling; Heterologous expression; Geobacter sulfurreducens; PpcA
Strategies for producing recombinant sucrose phosphorylase originating from Bifidobacterium longum in Escherichia coli JM109
Keywords: بیان هترولوژیک; Sucrose phosphorylase; Bifidobacterium longum; Escherichia coli; Plasmid stability; Heterologous expression; Inclusion body
Phytate utilization by genetically engineered lysine-producing Corynebacterium glutamicum
Keywords: بیان هترولوژیک; PSM, phytase screening medium; WBE, wheat bran extract; Pi, inorganic phosphateCorynebacterium glutamicum; Phytase; Phytic acid utilization; Heterologous expression; Protein secretion; Lysine production
Heterologous expression of the alcohol dehydrogenase (adhI) gene from Geobacillus thermoglucosidasius strain M10EXG
Keywords: بیان هترولوژیک; Thermophile; Geobacillus thermoglucosidasius; Thermophilic alcohol dehydrogenase; Ethanol production; Heterologous expression
Heterologous expression, purification and characterisation of the extracellular domain of trypanosome invariant surface glycoprotein ISG75
Keywords: بیان هترولوژیک; Trypanosoma brucei; Recombinant ISG75; Heterologous expression; Stabilisation agents; Sera
Cloning, sequence analysis and heterologous expression in Pichia pastoris of a gene encoding a thermostable cellobiose dehydrogenase from Myriococcum thermophilum
Keywords: بیان هترولوژیک; Cellobiose dehydrogenase; GMC-flavoenzyme superfamily; Heterologous expression; Pichia pastoris
Production of a recombinant industrial protein using barley cell cultures
Keywords: بیان هترولوژیک; Collagen; Heterologous expression; Barley cell culture; Hordeum vulgare
High-level expression, purification and characterization of recombinant Aspergillus oryzae alkaline protease in Pichia pastoris
Keywords: بیان هترولوژیک; Alkaline protease; Aspergillus oryzae; Pichia pastoris; Heterologous expression; Purification
Molecular cloning and characterization of O-methyltransferases from the flower buds of Iris hollandica
Keywords: بیان هترولوژیک; cDNA cloning; Heterologous expression; Iris hollandica; O-methyltransferase; Phenylpropanoid biosynthesisCOMT, caffeic acid 3-OMT; delphinidin 3pCRG, delphinidin 3-(p-coumaroyl)rhammnosylglucoside; delphinidin 3pCRG5G, delphinidin 3-(p-coumaroyl)rhammnosy
Molecular gene cloning and overexpression of the phytase from Debaryomyces castellii CBS 2923
Keywords: بیان هترولوژیک; Gene isolation; Phytase; Debaryomyces castellii; Pichia pastoris; Heterologous expression
Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA supply
Keywords: بیان هترولوژیک; Acetyl-CoA carboxylase; Malonyl-CoA; Polyketides; Heterologous expression; Precursor supply
Characterization of a new antifungal lipid transfer protein from wheat
Keywords: بیان هترولوژیک; Heterologous expression; Lipid transfer protein; Antifungal activity; Transgenic tobaccoLTPs, lipid transfer proteins; PR, pathogenesis-related; cDNA, complementary DNA; 8 CM, eight-cysteine motif; IPTG, isopropyl β-thiogalactoside; PBS, phosphate-buffere
Cloning and functional characterization of BcatrA, a gene encoding an ABC transporter of the plant pathogenic fungus Botryotinia fuckeliana (Botrytis cinerea)
Keywords: بیان هترولوژیک; Gene expression; Heterologous expression; Multidrug resistance; PDR5; Protein encoding;
Alternative pig liver esterase (APLE) – Cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst
Keywords: بیان هترولوژیک; Biocatalysis; Pig liver esterase; Kinetic resolution; Heterologous expression; Pichia pastoris; Periplasm
Three mutations identified in the voltage-sensitive sodium channel α-subunit gene of permethrin-resistant human head lice reduce the permethrin sensitivity of house fly Vssc1 sodium channels expressed in Xenopus oocytes
Keywords: بیان هترولوژیک; Heterologous expression; Human head lice; Knockdown resistance; Pediculus humanus capitis; Permethrin resistance; Voltage-sensitive sodium channelDDT, dichlorodiphenyltrichloroethane; kdr, knockdown resistance; PCR, polymerase chain reaction; Vssc1, volta
Characterization and cloning of chitin deacetylases from Rhizopus circinans
Keywords: بیان هترولوژیک; Chitin deacetylase; Enzyme; Gene expression; Heterologous expression; Protein engineering;
Recombinant expression of twelve evolutionarily diverse subfamily Iα aminotransferases
Keywords: بیان هترولوژیک; Aminotransferase; Pyridoxal phosphate; Intein; Heterologous expression; Substrate specificity; Annotation
Isolation of two laccase genes from the white-rot fungus Pleurotus eryngii and heterologous expression of the pel3 encoded protein
Keywords: بیان هترولوژیک; Basidiomycete; Phenol oxidases; Copper protein; Protein sequence; Heterologous expression
Characterisation of novel defective thiopurine S-methyltransferase allelic variants
Keywords: بیان هترولوژیک; TPMT genetic polymorphism; Thiopurine drugs; Heterologous expression; HPLC; Molecular modeling
Purification and biochemical characterization of a novel α-amylase from Bacillus licheniformis NH1: Cloning, nucleotide sequence and expression of amyN gene in Escherichia coli
Keywords: بیان هترولوژیک; α-Amylase; amyN gene; Bacillus licheniformis; Escherichia coli; Heterologous expression; Thermostability
Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering
Keywords: بیان هترولوژیک; Streptomyces; Metabolism; Antibiotic biosynthesis; Precursor engineering; Heterologous expression; Regulation; Ribosome engineering; Genome shuffling