Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Reconstitution; Biomembranes; Lipids; Detergents; Unilamellar vesicles; Lipid bilayer; Nanodiscs; Liposomes;
مقالات ISI پروتئین غشایی انتگرال (ترجمه نشده)
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Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Phase separation; Polyethylene glycol; Salt; Stability
Keywords: پروتئین غشایی انتگرال; Detergent; Integral membrane protein; Polyethylene glycol; Precipitation curve; Salt; BisTris; Bis(2-hydroxyethyl) iminotris-(hydroxymethyl) methane; C12E8; N-dodecyl-octaoxyethylene; LDAO; N,N-dimethyldodecylamine-N-oxide; LM; N-dodecylâ
Keywords: پروتئین غشایی انتگرال; Automation; Eukaryotic cell-free protein synthesis; Integral membrane protein; Non-canonical amino acid; Electrophysiology; Fluorescence modification; Amb210; amber stop codon at position corresponding to amino acid 210 in translated protein; AzF; p-Azido
Keywords: پروتئین غشایی انتگرال; RPA; resistance to protein adsorption; OEO; oligo(ethylene oxide); TLC; thin layer chromatography; SAM; self-assembled monolayer; AAO; anodized aluminum oxide; IMP; integral membrane protein; FBS; fetal bovine serum; Fb; fibrinogen; Rd; rhodopsin; CA; con
Keywords: پروتئین غشایی انتگرال; IMP; integral membrane protein; GFP; green fluorescent protein; FSEC; fluorescence-detected size-exclusion chromatography; IMAC; immobilized metal affinity chromatography purification; SEC; size-exclusion chromatography; GF; gel filtration; AGF; analytica
Optimising the transient expression of GABA(A) receptors in adherent HEK293 cells
Keywords: پروتئین غشایی انتگرال; GABAA receptor; Ligand-gated ion channel; Transient expression; HEK293; IMP; integral membrane protein; BacMam; baculovirus infection of mammalian cells; CaP; calcium phosphate; PEI; polyethyleneimine; SV40; Simian vacuolating virus 40; SV40-ori; SV40 ori
Intramembranal disulfide cross-linking elucidates the super-quaternary structure of mammalian CatSpers
Keywords: پروتئین غشایی انتگرال; Electrophysiology; Integral membrane protein; Sperm; Contraception; Hyperactive motility; sperm competition; Disulfide linkage; Bioinformatics; Mechanotransduction; Disulfide isomerase;
Purification and characterization of the colicin A immunity protein in detergent micelles
Keywords: پروتئین غشایی انتگرال; BS3; bis(sulfosuccinimidyl)suberate; C12E8; dodecyl octaethylene glycol ether; C12E9; dodecyl nonaethylene glycol ether; CD; circular dichroism; Cholate; 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid, monosodium salt; CHAPS; 3-[(3-cholamidopropyl)-dimeth
Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and function
Keywords: پروتئین غشایی انتگرال; AMPPCP; β, γ-methylene ATP; CD; circular dichroism; CU; cooperative unit; CFTR; cystic fibrosis transmembrane conductance regulator; DDM; n-dodecyl-β-d-maltopyranoside; ÎHc; calorimetric enthalpy; DLS; dynamic light scattering; DMNG; decyl maltose neo
Mechanistic analysis of ghrelin-O-acyltransferase using substrate analogs
Keywords: پروتئین غشایی انتگرال; Ghrelin; Ghrelin-O-acyltransferase; GOAT; Enzymology; Mechanism; Integral membrane protein; Solubilization
Expression and purification of integral membrane metallopeptidase HtpX
Keywords: پروتئین غشایی انتگرال; Metallopeptidase; Integral membrane protein; Protein quality control; Escherichia coli; Overexpression
Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies
Keywords: پروتئین غشایی انتگرال; GPCR; G protein-coupled receptor; IMP; integral membrane protein; CHESS; cellular high-throughput encapsulation solubilization and screening; NT; neurotensin peptide; FACS; fluorescence-activated cell sorting; EDTA; ethylenediaminetetraacetic acid; DDM; n
Expression and purification of recombinant Saccharomyces cerevisiae mitochondrial carrier protein YGR257Cp (Mtm1p)
Keywords: پروتئین غشایی انتگرال; Mitochondrial carrier protein; Integral membrane protein; Initiation codon; Codon bias; Rare tRNA; Pyridoxal 5â²-phosphate;
Prefractionation and separation by C8 stationary phase: Effective strategies for integral membrane proteins analysis
Keywords: پروتئین غشایی انتگرال; Reverse phase stationary phase; Hydrophobic peptides; Integral membrane protein; Prefractionation; μRPLC-ESI-MS/MS;
Reconstitution of integral membrane proteins into isotropic bicelles with improved sample stability and expanded lipid composition profile
Keywords: پروتئین غشایی انتگرال; NMR; nuclear magnetic resonance; DM; decylmaltoside; DHPC; 1,2-dihexanoyl-sn-glycero-3-phosphocholine; DLPC; 1,2-dilauroyl-sn-glycero-3-phosphocholine; DMPC; 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC; 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; P
Lipid-protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes
Keywords: پروتئین غشایی انتگرال; Brij-35; dodecylpolyoxyethylene 35; Brij-58; hexadecylpolyoxyethylene 58; Brij-78; octadecylpolyoxyethylene 78; Brij-98; octadecylpolyoxyethylene 98; CF; cell-free; DC7PC; 1,2-diheptanoyl-sn-glycero-3-phosphocholine; DHPC; 1,2-dihexanoyl-sn-glycero-3-phos
Functional and biochemical characterisation of the Escherichia coli major facilitator superfamily multidrug transporter MdtM
Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Antibiotic resistance; Multidrug efflux; Major facilitator superfamily; Ethidium bromide efflux assays; BCA; bicinchoninic acid; BSA; bovine serum albumin; CCCP; carbonyl cyanide 3-chlorophenylhydrazone; DDM; dodecyl-β-d-maltop
Biophysical characterisation of the Chlamydia pneumoniae integral membrane protein IncC in detergent micelles
Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Inclusion membrane protein; IncC; Chlamydia pneumoniae; NMR;
The phosphoenolpyruvate-dependent glucose–phosphotransferase system from Escherichia coli K-12 as the center of a network regulating carbohydrate flux in the cell
Keywords: پروتئین غشایی انتگرال; Bacterial phosphotransferase system; PTS; Glucose; Carbohydrate transport; Integral membrane protein; Gene regulation; Catabolite repression; Metabolic balance; Modeling; ptsG; Mlc; MtfA; SgrT
Early targeting events during membrane protein biogenesis in Escherichia coli
Keywords: پروتئین غشایی انتگرال; Signal recognition particle; SRP; SRP-receptor; SRP-RNA; Ffh; FtsY; 4.5S RNA; Escherichia coli; Integral membrane protein; Ribosome; mRNA targeting;
Triton X-114 phase separation in the isolation and purification of mouse liver microsomal membrane proteins
Keywords: پروتئین غشایی انتگرال; TX-114; Triton X-114; IMP; integral membrane protein; IPI; International Protein Index; MF; microsome fraction; DP; detergent phase; AP; aqueous phase; PF; pellet fraction; TMHMM; transmembrane hidden markov model; GRAVY; grand average of hydropathy; TMD;
High-yield expression and purification of the transmembrane region of ion channel-forming amyloid-β protein for NMR structural studies
Keywords: پروتئین غشایی انتگرال; Amyloid channel; Integral membrane protein; Transmembrane protein; Multimeric coding sequence; Alzheimer's disease; NMR
Testicular cell adhesion molecule 1 (TCAM1) is not essential for fertility
Keywords: پروتئین غشایی انتگرال; Spermatocyte; Male fertility; Integral membrane protein; Transgenic mice
Systematic cyanobacterial membrane proteome analysis by combining acid hydrolysis and digestive enzymes with nano-liquid chromatography–Fourier transform mass spectrometry
Keywords: پروتئین غشایی انتگرال; Acid hydrolysis/chymotrypsin; Acid hydrolysis/trypsin; Synechocystis sp. PCC 6803; Trans-membrane domain; Integral membrane protein; HPLC; nano-LC–MS
Improvement of hydrophobic integral membrane protein identification by mild performic acid oxidation-assisted digestion
Keywords: پروتئین غشایی انتگرال; Mild performic acid oxidation; Hydrophobic; Integral membrane protein; Mass spectrometry
Functional expression, purification and high sequence coverage mass spectrometric characterization of human excitatory amino acid transporter EAAT2
Keywords: پروتئین غشایی انتگرال; Glutamate; Membrane transporter; Excitatory amino acid transporter; Integral membrane protein; Protein digestion; Mass spectrometry; Peptide mapping; MALDI-TOF;
Plasma membrane tubulin
Keywords: پروتئین غشایی انتگرال; Microtubule; Integral membrane protein; Linker; Electrostatic; Receptor; Channel
Expression and characterization of the integral membrane domain of bacterial histidine kinase SCO3062 for structural studies
Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Hemoglobin fusion; Histidine kinase; SCO3062; Nuclear magnetic resonance; NMR
Sodium-deoxycholate-assisted tryptic digestion and identification of proteolytically resistant proteins
Keywords: پروتئین غشایی انتگرال; Proteolytical resistance; Sodium deoxycholate (SDC); Trypsin; Digestion; Identification; Myoglobin; Integral membrane protein
Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex
Keywords: پروتئین غشایی انتگرال; Cell-free translation; Wheat germ; Human stearoyl-CoA desaturase; Integral membrane protein; Human cytochrome b5; Human cytochrome b5 reductase; Purification; Catalytic assay; Liposome
Mass spectrometry of full-length integral membrane proteins to define functionally relevant structural features
Keywords: پروتئین غشایی انتگرال; Mass spectrometry; Integral membrane protein; Structure–function relationship; Limited proteolysis; Protocol
Analysis of a G protein-coupled receptor for neurotensin by liquid chromatography–electrospray ionization–mass spectrometry
Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Intact protein; Detergents; Electrospray ionization–mass spectrometry (ESI–MS); G protein-coupled receptor (GPCR); Neurotensin receptor
High yield cell-free production of integral membrane proteins without refolding or detergents
Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Transporter; Cell-free protein synthesis; In vitro protein synthesis
The role of tryptophan cation–pi interactions on ammonia transport through the AmtB ammonia channel
Keywords: پروتئین غشایی انتگرال; Ammonia channel; AmtB; Integral membrane protein; X-ray crystallography; Cation–pi interactions; 2006 Tokyo ISTRY Conference
Archaeal N-terminal Protein Maturation Commonly Involves N-terminal Acetylation: A Large-scale Proteomics Survey
Keywords: پروتئین غشایی انتگرال; NAT, N-terminal acetyltransferase; MAP, methionine aminopeptidase; MS, peptide mass fingerprint analysis; MS/MS, tandem mass spectrometry; SCX, strong cation exchange chromatographyN-terminal acetylation; methionine cleavage; integral membrane protein; ma
Transmembrane domain prediction and consensus sequence identification of the oligopeptide transport family
Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Motifs; Amino acid; Sequence homology determination;
Isolation of integral membrane proteins of Leishmania promastigotes and evaluation of their prophylactic potential in hamsters against experimental visceral leishmaniasis
Keywords: پروتئین غشایی انتگرال; Integral membrane protein; Triton X-114; Phase separation; Vaccination; Hamster; Leishmania donovani; Antibody response; LTT;
Identification of an integral plasma membrane pool of protein kinase C in mammalian tissues and cells
Keywords: پروتئین غشایی انتگرال; PKC; Integral membrane protein; Plasma membrane; Phorbol ester; Diacylglycerol;