Biophysical characterisation of the Chlamydia pneumoniae integral membrane protein IncC in detergent micelles

The cross-linking and AUC data indicate that IncC-Full and IncC-TM form homo-dimers in DPC micelles. Based on NMR T1 and T2 relaxation, we estimate the molecular mass of IncC-Full and IncC-TM in DPC micelles to be approximately 60.2 and 42.7 kDa, respectively, while the calculated AUC molecular masses in the buoyant density of the detergent were 37.6 kDa and 14.7 kDa, respectively. The 3-D 1H-15N-1H NOESY-HSQC data indicate that both IncC-Full and IncC-TM are primarily alpha helical structures.