Keywords: Lac operon; Bistability; TMG; IPTG; Root locus
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A modified clear-native polyacrylamide gel electrophoresis technique to investigate the oligomeric state of MBP-5-HT3A-intracellular domain chimeras
Keywords: Clear-native PAGE; Multi-angle light scattering; Native gel electrophoresis; Protein oligomerization; Protein quaternary structures; Size exclusion chromatography; 5-HT3A; serotonin type 3A; 6-AHA; 6-aminohexanoic acid; BN-PAGE; blue-native polyacrylamide
Construction of pOGOduet - An inducible, bicistronic vector for synthesis of recombinant proteins in Corynebacterium glutamicum
Keywords: Corynebacterium glutamicum; Duet-expression; Plasmid; CMN; Corynebacterium-Mycobacterium-Nocardia; WT; wild-type; IPTG; Isopropyl β-D-1-thiogalactopyranoside; aTC; Anhydrotetracycline; MCS; Multiple Cloning Site;
Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein
Keywords: Escherichia coli; Short peptide; Ultraviolet radiation; Late embryogenesis abundant protein; CFU; colony-forming units; IPTG; isopropylthio-d-galactoside; LB; Luria-Bertani medium; LEA; late embryogenesis abundant; OD600; optical density at 600â¯nm; UV
Autoubiquitination of feline E3 ubiquitin ligase BCA2
Keywords: BCA2; breast cancer associated gene 2; RNF115; ring finger protein 115; BZF; BCA2 zinc finger; Mdm2; mouse double minute 2; BRCA1; breast cancer 1; FMC; feline mammary carcinoma; WHO; World Health Organization; ATCC; American Type Culture Collection; BCIP
Introduction of an extra tryptophan fluorophore by cataract-associating mutations destabilizes βB2-crystallin and promotes aggregation
Keywords: βB2-crystallin; Congenital hereditary cataract; Protein aggregation; Protein folding; Trp fluorophore; UV damage; ANS; 1-anilinonaphtalene-8-sulfonate; CD; circular dichroism; DTT; dithiothreitol; Emax; maximum emission wavelength of fluorescence; IPTG;
Optimization of Haemophilus influenzae adhesin transmembrane domain expression in Escherichia coli
Keywords: Outer membrane protein; Recombinant protein expression; Trimeric autotransporter; BAM; β-barrel assembly machinery; HEPES; 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid; HRP; horseradish peroxidase; HiaTD; Haemophilus influenzae adhesin transm
Rapid NMR-scale purification of 15N,13C isotope-labeled recombinant human STIM1 coiled coil fragments
Keywords: Signal protein; Oligomerization; Calcium channel; Coiled coil; NMR spectroscopy; STIM1; stromal interaction molecule 1; SOCE; store operated calcium entry; ER; endoplasmic reticulum; PM; plasma membrane; CRAC; calcium release-activated channels; NMR; nucl
Identification and characterization of GH62 bacterial α-l-arabinofuranosidase from thermotolerant Streptomyces sp. SWU10 that preferentially degrades branched l-arabinofuranoses in wheat arabinoxylan
Keywords: AcB; sodium acetate buffer; AFase; α-l-arabinofuranosidase; AXH; arabinoxylan arabinofuranohydrolase; GH; glycoside hydrolase; HPAEC; high-performance anion-exchange chromatography; IPTG; isopropyl-β-d-thiogalactopyranoside; KPB; potassium phosphate buf
Cloning and characterization of a novel phenylalanine ammonia-lyase gene from Inonotus baumii
Keywords: HPLC; high-performance liquid chromatography; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; DMW; dry mycelium weight; DOP-PCR; degenerate oligonucleotide primer PCR; RACE-PCR; rapid-amplification of cDNA ends PCR; vgb; Vitreoscilla
LEA proteins from Gastrodia elata enhance tolerance to low temperature stress in Escherichia coli
Keywords: LEA protein; late embryogenesis abundant protein; IPTG; isopropyl β-d-thiogalactopyranoside; Gastrodia elata; LEA; Abiotic stress;
Expression of hNeuritin protein in a baculovirus expression system and the analysis of its activity
Keywords: SDS-PAGE; Sodium dodecyl sulfate- polyacrylamide gel electrophoresis; FBS; Fetal calf serum; DRG; Dorsal root ganglia; IPTG; Isopropyl β-D-Thiogalactoside; X-Gal; 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside; RT-PCR; Reverse transcription-PCR; DMEM;
Dimethylsulfoniopropionate biosynthesis in a diatom Thalassiosira pseudonana: Identification of a gene encoding MTHB-methyltransferase
Keywords: Algae; Diatom; Dimethylsulfoniopropionate; Compatible solute; MTHB methyltransferase; Thalassiosira pseudonana; ASW; artificial sea water; DMSHB; 4-dimethylsulfonio-2-hydroxy-butyrate; DMSP; dimethylsulfoniopropionate; GB; glycine betaine; IPTG; isopropyl
Amputation of a C-terminal helix of the γ subunit increases ATP-hydrolysis activity of cyanobacterial F1 ATP synthase
Keywords: FoF1; FoF1 ATP synthase; T. elongatus; Thermosynechococcus elongatus BP-1; E. coli; Escherichia coli; PCR; polymerase chain reaction; IPTG; isopropyl-β-d-thiogalactopyranoside; HEPES; N-2-hydroxyl piperazine-Nâ²-2-ethane sulfonic acid; LDAO; lauryl dime
Molecular characterization and in silico analysis of ectoine biosynthesis genes from the deep sea halophilic eubacteria, Bacillus clausii NIOT-DSB04
Keywords: ectA, B & C; ectoine biosynthesis genes; IPTG; isopropyl-β-d-thiogalactoside; PDB; Protein Data Bank; Bacillus clausii; Halophilic bacteria; Compatible solutes; Ectoine genes; Osmoregulation;
Recombinant latcripin 11 of Lentinula edodes C91-3 suppresses the proliferation of various cancer cells
Keywords: CCK-8; cell counting kit-8; DPPH; 1,1-dipheny l-2-picrylhydrazyl; A549; human lung adenocarcinoma epithelial cell; Hep-2; human epithelial cell; HL-60; human promyelocytic leukemia cell; HUVEC; human umbilical vein endothelial cell; IPTG; isopropyl β-d-t
Heterologous expression and antimicrobial activity of OsGASR3 from rice (Oryza sativa L.)
Keywords: AMP; antimicrobial peptide; GASR; gibberellic acid, stimulated transcript-related; GST; glutathione S-transferase; MIC; minimum inhibitory concentration; IPTG; isopropyl β-D-1-thiogalactopyranoside; PCR; polymerase chain reaction; PDA; potato dextrose ag
Generation of a T cell receptor (TCR)-like single domain antibody (sDAb) against a Mycobacterium Tuberculosis (Mtb) heat shock protein (HSP) 16kDa antigen presented by Human Leukocyte Antigen (HLA)-A*02
Keywords: ABTS; 2,2â²-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt; APC; Antigen presenting cells; APD; Antigen Phage Display; BSA; bovine serum albumin; DAb; human domain antibody; ELISA; enzyme-linked immunosorbent assays; Fv; Fragment varia
The N-terminal domains of Bacillus subtilis CopA do not form a stable complex in the absence of their inter-domain linker
Keywords: CD; Circular dichroism; DTNB; 5,5â²-dithiobis-(2-nitrobenzoic acid); DTT; dithiothreitol; ESI-MS; electrospray ionization mass spectrometry; Hepes; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; HSQC; heteronuclear single quantum correlation; ICP-AE
Heterologous expression and enhanced production of β-1,4-glucanase of Bacillus halodurans C-125 in Escherichia coli
Keywords: Bacillus halodurans; Cellulases; Cellulose hydrolysis; Degradation of cellulose; Endo-1; 4-β-glucanases; Expression analysis; Heterologous expression; In silico protein characterization; IPTG; pET expression system; Plant cell wall;
The critical role of porcine cytochrome P450 3A46 in the bioactivation of aflatoxin B1
Keywords: AFBO-GSH; AFB1-8,9-epoxide glutathione; ALA; δ-aminolevulinic acid; BHA; butylated hydroxyanisole; DLPC; 1,2-didodecanoyl-sn-glycero-3-phosphocholine; DOPC; 1,2-Dioleoyl-sn-glycero-3-phosphocholine; DOPS; 1,2-dilauroyl-sn-glycero-3-phospho-l-serine; DTT;
Simultaneous biosynthesis of (R)-acetoin and ethylene glycol from D-xylose through in vitro metabolic engineering
Keywords: EG; ethylene glycol; PET; polyethylene terephthalate; ThDP; Thiamine diphosphate; FAD; flavin adenine dinucleotide; NAD+; oxidized nicotinamide adenine dinucleotide; NADH; reduced nicotinamide adenine dinucleotide; LB; lysogeny broth; IPTG; isopropyl-β-D
Steroids from Ganoderma sinense as new natural inhibitors of cancer-associated mutant IDH1
Keywords: IDH1; Steroids; Ganoderma sinense; Virtual ligand screening, Cancer; IDH1; isocitrate dehydrogenase; MST; microscale thermophoresis; CCK8; cell counting Kit-8; IPTG; isopropyl β-D-1-thiogalactopyranoside; PMSF; phenylmethanesulfonyluoride; PVDF; polyviny
Porcine enterocyte protein Btnl5 negatively regulates NF-kappa B pathway by interfering p65 nuclear translocation
Keywords: Btnl5; butyrophilin-like 5; IECs; intestinal epithelial cells; TRAF2; TNF receptor-associated factor 2; Btn; Butyrophilin; Btnl; butyrophilin-like; Skint; selection and upkeep of intraepithelial T cells; IκB; inhibitory κB; IPTG; isopropy-β-d-thiogalac
Molecular cloning and characterization of C1 tetrahydrofolate (C1-THF) synthase in Bombyx mori, silkworm
Keywords: THF; C1 tetrahydrofolate; silkworm cytoplasmic C1-THF synthase; BmC1-THF synthase; ORF; open reading frame; qRT-PCR; quantitative real-time PCR; THF_DHG_CYH; methylenetetrahydrofolate dehydrogenase; THF_DHG_CYH_C; methenyltetrahydrofolate cyclohydrolase;
A Lysin motif (LysM)-containing protein from Hong Kong oyster, Crassostrea hongkongensis functions as a pattern recognition protein and an antibacterial agent
Keywords: cDNA; DNA complementary to RNA; RNA; Ribonucleic acid; DNA; deoxyribonucleic acid; kDa; Kilodalton; nt; nucleotide; s; second; h; hour; NCBI; National Center for Biotechnology Information; SMART; Simple Modular Architecture Research Tool; EF1α; elongatio
Combination of novel DR5 targeting agonistic scFv antibody TR2-3 with cisplatin shows enhanced synergistic antitumor activity in vitro and in vivo
Keywords: DR5; death receptor 5; DR4; death receptor 4; TNF; tumor necrosis factor; TRAIL; TNF-related apoptosis inducing ligand; scFv; single chain fragment variable; MTT; methyl thazoy terazolium; IPTG; isopropyl β-D-1-thiogalactopyranoside; PBS; phosphate buffe
Babesia microti thioredoxin 3 is an effective antioxidant and involved in the response to antiprotozoal drugs
Keywords: Trx; thioredoxin; TrxR; thioredoxin reductase; NADPH; nicotinamide adenine dinucleotide phosphate; BmTrx3; B. microti thioredoxin 3; RACE; rapid amplification of cDNA ends; IFA; Immunofluorescence assay; IPTG; Isopropyl β-d-1-Thiogalactopyranoside; PBS;
Anticancer activity profiling of parthenolide analogs generated via P450-mediated chemoenzymatic synthesis
Keywords: PTL; parthenolide; AML; acute myelogenous leukemia; CSCs; cancer stem cells; DMAPT; dimethylamino-parthenolide; PTDH; phosphite dehydrogenase; NADPH; nicotinamide adenine dinucleotide phosphate (reduced); IPTG; β-d-1-thiogalactopyranoside; δ-ALA; δ-ami
Pseudomonas aeruginosa overexpression system of nitric oxide reductase for in vivo and in vitro mutational analyses
Keywords: NOR; nitric oxide reductase; HCO; heme copper oxidase; cNOR; cytochrome c dependent NOR; IPTG; isopropyl-1-thio-β-d-galactopyranoside; DDM; dodecyl-β-d-maltoside; AAS; atomic absorption spectroscopy; PMS; phenazine methosulfate; qNOR; quinol-dependent N
Cloning, overexpression, and purification of a gene of unknown function of prophage loci from 'Candidatus Liberibacter asiaticus,' the destructive bacterial pathogen of huanglongbing disease in citrus plants
Keywords: Candidatus Liberibacter asiaticus'; Huanglongbing disease; Blotchy mottle; Lime; Type-D; Prophage; CLas; 'Candidatus Liberibacter asiaticus'; E. coli; Escherichia coli; A. tumefaciens; Agrobacterium tumefaciens; A. thaliana; Arabidopsis thaliana; C.
A novel multi-peptide subunit vaccine admixed with AddaVax adjuvant produces significant immunogenicity and protection against Proteus mirabilis urinary tract infection in mice model
Keywords: UTI; urinary tract infection; CAUTI; catheter-associated urinary tract infection; MR/P; mannose-resistant Proteus-like fimbriae; UCA; uroepithelial cell adhesin; Pta; proteus toxic agglutinin; MUP; MrpA UcaA and Pta; GPGPG; Gly-Pro-Gly-Pro-Gly; MHC; major
Characterization of l-aspartate oxidase from Arabidopsis thaliana
Keywords: DE; dicarboxylic exchangers; FAD; flavin adenine dinucleotide; FADH2; FAD hydroquinone form; GST; glutathione S-transferase; iminoasp.; iminoaspartate; IPTG; isopropyl thio-β-d-galactoside; LASPO; l-aspartate oxidase; NAD+; nicotinamide adenine dinucleot
A serine carboxypeptidase-like acyltransferase catalyzes synthesis of indole-3-acetic (IAA) ester conjugate in rice (Oryza sativa)
Keywords: Auxin conjugate; serine carboxypeptidase-like acyltransferase; Indole-3-acetic acid; Indole-3-acetyl-myo-inositol; Oryza sativa; 1-O-IAGlc; 1-O-indole-3-acetyl-β-D-glucose; GSH; reduced glutathione; GSSH; oxidized glutathione; IAA; indole-3-acetic acid;
Cooperative binding promotes demand-driven recruitment of AnxA8 to cholesterol-containing membranes
Keywords: Anx; annexin; AnxA8ins; siRNA-insensitive AnxA8; Ctrl; control; GFP; green fluorescent protein; IPTG; isopropyl-β-d-thiogalactose; LDL; low-density lipoprotein; LEL; late endosomes/lysosomes; NPC1; Niemann Pick disease type C1; PM; plasma membrane; PNS;
Topography of human cytochrome b5/cytochrome b5 reductase interacting domain and redox alterations upon complex formation
Keywords: Cytochrome b5; Cb5; Cytochrome b5 reductase; Cb5R; Cytochrome c; Cyt c; Cytoglobin; Cyg; Ethylene diamine tetra acetic acid; EDTA; Flavin adenine dinucleotide; FAD; Heteronuclear single quantum coherence spectroscopy; HSQC; Isopropyl Ã-D-1-thiogalactopyr
Structural and functional insights into RHA-P, a bacterial GH106 α-L-rhamnosidase from Novosphingobium sp. PP1Y
Keywords: Rhamnosidase; Flavonoid; Homology modeling; Site-directed mutagenesis; Novosphingobium sp. PP1Y; α-RHA; α-l-rhamnosidase; ABS; absorbance; BSA; bovin serum albumin; E. coli; Escherichia coli; GHs; glycosyl hydrolases; GTs; glycosyl transferases; IPTG; i
Comparison of six cell penetrating peptides with different properties for in vitro and in vivo delivery of HPV16 E7 antigen in therapeutic vaccines
Keywords: HPV; human papillomavirus; CPP; cell penetrating peptide; RP-HPLC; reversed-phase high performance liquid chromatography; r; recombinant; IPTG; isopropyl-β--thiogalactopyranoside; ConA; concanavalin A; Human papillomavirus; E7 protein; Cervical cancer; C
The antitumor effect of TAT-DCF1 peptide in glioma cells
Keywords: GBM; glioblastoma; DCF1; dendritic cell-derived factor 1; TAT; transactivator of transcription; IPTG; isopropyl-β-thiogalactopyranoside; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; RT; room temperature; PBS; phosphate buffered sa
The aldehyde dehydrogenase AldA contributes to the hypochlorite defense and is redox-controlled by protein S-bacillithiolation in Staphylococcus aureus
Keywords: ADH; aldehyde dehydrogenase; BSH; bacillithiol; BSSB; oxidized bacillithiol disulfide; CFU; colony-forming unit; CD; catalytic domain; Co-BD; coenzyme-binding domain; DTT; dithiothreitol; EDTA; ethylenediaminetetraacetic acid; FA; formaldehyde; H2O2; hydr
Crystal structure and substrate specificity of ExoY, a unique T3SS mediated secreted nucleotidyl cyclase toxin from Pseudomonas aeruginosa
Keywords: X-ray crystallography; Nucleotidyl cyclase toxins; T3SS; Anthrax toxA family; Non-canonical secondary messengers; One-metal-ion dependent catalysis; ExoY; exogenous toxin Y; T3SS; Type III secretory systems; AA-NC; actin-activated nucleotidyl cyclase; EF;
The parasite-derived rOv-ASP-1 is an effective antigen-sparing CD4+ T cell-dependent adjuvant for the trivalent inactivated influenza vaccine, and functions in the absence of MyD88 pathway
Keywords: HA; Hemagglutinin; IMAC; Immobilized Metal Affinity Chromatography; IPTG; Isopropyl β-D-1-thiogalactopyranoside; rOv-ASP-1; Recombinant Onchocerca volvulus activation associated protein-1; Th cells; T helper cells; T-PER; Tissue protein extraction reagen
Vaccination with a CD4+ and CD8+ T-cell epitopes-based recombinant chimeric protein derived from Leishmania infantum proteins confers protective immunity against visceral leishmaniasis
Keywords: BM; Bone marrow; CD4; Cluster of differentation 4; CD8; Cluster of differentation 8; dLN; Draining lymph nodes; DO; Optical density; ELISA; Enzyme-linked immunosorbent assay; GM-CSF; Granulocyte-macrophage colony-stimulating factor; GRAVY; aliphatic index
Detection of ciprofloxacin residues in cow milk: A novel and rapid optical β-galactosidase-based screening assay
Keywords: ATCC; American Type Culture Collection; β-gal; β-galactosidase; IPTG; isopropyl-β-d-1-tiogalattopiranoside; LB; Luria Bertani; CFU; Colony Forming Unit; MRL; Maximum Residual Limit; OD; Optical Density; ONPG; O-nitrophenyl-β-d-galactopyranoside; X-gal
The Shewanella woodyi galactokinase pool phosphorylates glucose at the 6-position
Keywords: Galactokinase; Hexokinase; GHMP family; Shewanella woodyi; ADP; adenosine diphosphate; ATP; adenosine triphosphate; EDTA; ethylenediamine tetraacetic acid; G6P; glucose-6-phosphate; G6PDH; glucose-6-phosphate dehydrogenase; GHMP; galactokinase, homoserine
Leptospira interrogans thermolysin refolded at high pressure and alkaline pH displays proteolytic activity against complement C3
Keywords: GdnHCl; guanidine hydrochloride; HHP; high hydrostatic pressure; IPTG; isopropyl β-D-thiogalactopyranoside; LB; Luria-Bertani broth; LS; light scattering; TL-IB; inclusion bodies of thermolysin; SDS-PAGE; sodium dodecyl sulphate polyacrylamide gel electr
Three novel variants (p.Glu178Lys, p.Val245Met, p.Ser250Phe) of the phenylalanine hydroxylase (PAH) gene impair protein expression and function in vitro
Keywords: BH4; Tetrahydrobiopterin; E. coli; Escherichia coli; ELISA; Enzyme-linked immunosorbent assay; hPAH; Human phenylalanine hydroxylase; IPTG; Isopropyl-β-d-thiogalactoside; kDa; Kilo Dalton; MBP; Maltose binding protein; PAH; Phenylalanine hydroxylase prot
Metalophilic lipase from Ralstonia solanacearum: Gene cloning, expression, and biochemical characterization
Keywords: LB; Luria-Baertani; p-NP; p-nitrophenyl; NCBI; National Centre of Biotechnology Information; IPTG; isopropyl-b-1-thiogalactopyranoside; DMSO; dimethysulfoxide; PMSF; phenylmethanesulfonyl; b-ME; b-mercaptoethanol; DDT; dithiothreitol; Ralstonia solanacear
Rapid purification of bacteriophage endolysin TSPphg and its exogenous treatment could act as an alternative bacterial cell disruption method
Keywords: Bacteriophage; Protein extraction; Host cell protein; Product recovery; IPTG; isopropyl-β-d-1-thiogalactopyranoside; AFM; atomic force microscopy; CFU; colony forming unit; TSPphg; peptidoglycan hydrolytic gene of Thermus siphoviridae phage 4;
Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression
Keywords: Hematopoietic cell kinase (HCK); X-ray crystallography; Cell-free protein synthesis; Solubility; Chaperone; Autophosphorylation; GFP; green fluorescent protein; CAT; chloramphenicol acetyl-transferase; HCK; hematopoietic cell kinase; TEV; tobacco etch vir