کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8288652 | 1536260 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dimethylsulfoniopropionate biosynthesis in a diatom Thalassiosira pseudonana: Identification of a gene encoding MTHB-methyltransferase
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کلمات کلیدی
IPTGSAMDMSPASWThalassiosira pseudonanaMTOBisopropyl-1-thio-β-d-galactopyranoside - 1-isopropyl-1-thio-β-d-galactopyranosideS-adenosyl-L-methionine - S-adenosyl-L-metionineartificial sea water - آب دریا مصنوعیAlgae - جلبک Compatible solute - حل سازگارDimethylsulfoniopropionate - دی متیل سولفونی پروپووناتdiatom - دیاتومMethyltransferase - متیل ترانسفرازGlycine betaine - گلیسین بتائین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Dimethylsulfoniopropionate biosynthesis in a diatom Thalassiosira pseudonana: Identification of a gene encoding MTHB-methyltransferase Dimethylsulfoniopropionate biosynthesis in a diatom Thalassiosira pseudonana: Identification of a gene encoding MTHB-methyltransferase](/preview/png/8288652.png)
چکیده انگلیسی
Dimethylsulfoniopropionate (DMSP) is one of the most abundant molecules on earth and plays a pivotal role in the marine sulfur cycle. DMSP is believed to be synthesized from methionine by a four-step reaction pathway in marine algae. The genes responsible for biosynthesis of DMSP remain unidentified. A diatom Thalassiosira pseudonana CCMP1335 is an important component of marine ecosystems and contributes greatly to the world's primary production. In this study, through genome search, in vivo activity and functional studies of cDNA products, a gene encoding Thalassiosira methyltransferase (TpMMT) which catalyzes the key step of DMSP synthesis formation of 4-methylthio-2-hydroxybutyrate (DMSHB) from 4-methylthio-2-oxobutyrate (MTHB), was identified. The amino acid sequence of TpMMT was homologous to the methyltransferase from Phaeodactylum tricornutum CCAP 1055/1, but not the recently identified bacterium gene. High salinity and nitrogen limitation stresses caused the increase of DMSP content and TpMMT protein in Thalassiosira. In addition to TpMMT, the enzyme activities for the first three steps could be detected and enhanced under high salinity, suggesting the importance of four-step DMSP synthetic pathway in Thalassiosira.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 645, 1 May 2018, Pages 100-106
Journal: Archives of Biochemistry and Biophysics - Volume 645, 1 May 2018, Pages 100-106
نویسندگان
Hakuto Kageyama, Yoshito Tanaka, Ayumi Shibata, Rungaroon Waditee-Sirisattha, Teruhiro Takabe,