Energetics of OCP1-OCP2 complex formation
Keywords: Ubiquitin ligase; SCF complex; F-box protein; Organ of Corti; Cochlea; ITC;
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Self-aggregation of hydrophobically modified dextrin and their interaction with surfactant
Keywords: Hydrophobically modified dextrin; Polymer–surfactant interaction; Self-aggregation; Calorimetry; ITC; Fluorescence
Thermodynamics of the binding of cytotoxic protoberberine molecule coralyne to deoxyribonucleic acids
Keywords: DNA; deoxyribonucleic acid; CP; Citrate-Phosphate; bp; base pair; ITC; isothermal titration calorimetry; DSC; differential scanning calorimetry; Tm; thermal melting; CD; circular dichroism; D/P; alkaloid/DNA molar ratio; P/D; DNA /alkaloid molar ratio;
Kinetic and thermodynamic characterization of dUTP hydrolysis by Plasmodium falciparum dUTPase
Keywords: dUTPase; dUTP pyrophosphatase (EC 3.6.1.23); α; β-imido-dUTP (or dUpNHpp), 2â²-deoxyuridine 5â²-(α,β-imido)triphosphate; ICP-MS; Inductively Coupled Plasma Mass Spectrometry; ITC; isothermal titration calorimetry; DSC; differential scanning calorime
Sequence specific and high affinity recognition of 5â²-ACGCGT-3â² by rationally designed pyrrole-imidazole H-pin polyamides: Thermodynamic and structural studies
Keywords: Binding; Biophysical; DNA; H-pin; Sequence selectivity; Polyamide; SPR; ITC;
Wavelet analysis of the EEG during the neurocognitive evaluation of invalidly cued targets
Keywords: P3a; P3b; Spectral power; ITC; Trial evaluation; Invalid cue
Thermodynamic contributions of the reactions of DNA intramolecular structures with their complementary strands
Keywords: Targeting nucleic acids; Intramolecular DNA structures; Antisense; Thermodynamics; ITC; DSC
Reactivity and plasticity in the amygdala nuclei during opiate withdrawal conditioning: Differential expression of c-fos and arc immediate early genes
Keywords: conditioned place aversion; in situ hybridization; basolateral complex; intercalated amygdala; aversive memories; opiate dependence; BLA; basolateral complex of the amygdala; CeA; central nucleus of the amygdala; CPA; conditioned place aversion paradigm;
Comparative evaluation of trimeric envelope glycoproteins derived from subtype C and B HIV-1 R5 isolates
Keywords: HIV; Trimer; Subtype C; CD4; Neutralizing antibodies; ITC
Bivalent peptides as PDZ domain ligands
Keywords: Peptide; Protein; ITC; Calorimetry; Multivalent; Bivalent; PDZ
Measurement of the second virial coefficient of DPPC- and DPPG-liposomes by isothermal titration calorimetry
Keywords: Second virial coefficient; Interaction potential; Liposome; ITC
Heat capacity changes in heme protein–ligand interactions
Keywords: Heat capacity changes; Heme protein; Ligand interactions; Conformational changes; ITC
Ap4A is not an efficient Zn(II) binding agent. A concerted potentiometric, calorimetric and NMR study
Keywords: Ap4A; Zn(II); Stability constants; Potentiometry; ITC; NMR
Interaction of insulin with a triblock copolymer of PEG-(fumaric-sebacic acids)-PEG: Thermodynamic and spectroscopic studies
Keywords: Copolymers; Insulin; ITC; CD; Acrylamide quenching; Denaturation;
Biophysical characterization of vaccinia virus thymidine kinase substrate utilization
Keywords: Orthopoxvirus; UV–vis spectrophotometry; Isothermal titration calorimetry; ITC; Binding thermodynamics; Substrate kinetics; Smallpox
Interactions between GSTM1, GSTT1 and GSTP1 polymorphisms and smoking and intake of fruit and vegetables in relation to lung cancer
Keywords: GST; glutathione S-transferase; ITC; isothiocyanate; GSTT1; GSTM1; GSTP1; Lung cancer; Polymorphism; Population-based;
Calibration in isothermal titration calorimetry: Heat and cell volume from heat of dilution of NaCl(aq)
Keywords: ITC; Calibration; Dilution enthalpy; NaCl(aq); Data analysis
Thermal unfolding of human BRCA1 BRCT-domain variants
Keywords: BRCA1-BRCT domain; Thermal unfolding; DSC; CD; ITC; V1833M; M1775R; R1699W;
Protonation Changes upon Ligand Binding to Trypsin and Thrombin: Structural Interpretation Based on pKa Calculations and ITC Experiments
Keywords: ITC, isothermal titration calorimetry; PB, Poisson–Boltzmann; PDLD, protein-dipole Langevin-dipole; Try, trypsin; Thr, thrombinprotonation states; pKa values; serine proteases; Poisson–Boltzmann; ITC
Complexation of integral membrane proteins by phosphorylcholine-based amphipols
Keywords: Amphipols; Integral membrane proteins; Phosphorylcholine; ITC; Amphiphilic macromolecules
Advances in the analysis of isothermal titration calorimetry data for ligand–DNA interactions
Keywords: Isothermal titration calorimetry; ITC; Data analysis; Binding model; Interactions; Thermodynamics; Biophysics; DNA; Ligand–DNA
Ca2+ and Mg2+ bind tetracycline with distinct stoichiometries and linked deprotonation
Keywords: TC; tetracycline; H4Tc+, H3Tc, H2Tcâ, HTc2â, and Tc3â; the different ionic forms of tetracycline; ITC; Isothermal titration calorimetry.; Isothermal titration calorimetry; Tetracycline metal ion interaction and stoichiometry; Linked deprotonation; I
Catabolism of Phenylalanine by Pseudomonas putida: The NtrC-family PhhR Regulator Binds to Two Sites Upstream from the phhA Gene and Stimulates Transcription with σ70
Keywords: EMSA, electrophoretic mobility-shift assay; IHF, integration host factor; ITC, isothermal titration calorimetry; LB, Luria Bertani culture medium; ORF, open reading frame; RT-PCR, reverse transcriptase-polymerase chain reactionNtrC family; PhhR; IHF; ITC;
Structure-specific recognition of quadruplex DNA by organic cations: Influence of shape, substituents and charge
Keywords: Synthetic cations; Telomere; DNA-quadruplex binding; G-tetrad; BIAcore; ITC;
Sequence and length dependent thermodynamic differences in heterocyclic diamidine interactions at AT base pairs in the DNA minor groove
Keywords: ITC; isothermal titration microcalorimetry; DSC; differential scanning calorimetry; SPR; surface plasmon resonance; CD; circular dichroism; Tm; thermal melting; kDNA; kinetoplast DNA; MES; 2-(N-morpholino) ethanesulfonic acid; RU; response units; DB75; Al
Ligand- and substrate-based 19F NMR screening: Principles and applications to drug discovery
Keywords: ALARM; a La assay to detect reactive molecules; BEI; binding efficiency index; CPMG; Carr-Purcell-Meiboom-Gill; CSA; chemical shift anisotropy; ERETIC; electronic reference to access in vivo concentrations; FA; fluorescence anisotropy; FABS; fluorin
RNA specific molecules: Cytotoxic plant alkaloid palmatine binds strongly to poly(A)
Keywords: Palmatine; RNA; Poly(A); Binding; Spectroscopy; ITC
Understanding the role of the G-actin-binding domain of Ena/VASP in actin assembly
Keywords: Actin nucleation; Elongation; ITC; WASP; Arp2/3 complex; Filopodia
Binding studies of hydantoin racemase from Sinorhizobium meliloti by calorimetric and fluorescence analysis
Keywords: ITC; Isothermal titration calorimetry; MTEH; Methylthioethyl hydantoin; SmeHyuA; hydantoin racemase from Sinorhizobium meliloti CECT 4114; ASA; Accessible surface area; HEPES; N-2-hydroxyethylpiperazine-Nâ²-2-ethanesulfonic acid; ACES; N-(2-Acetamido)-2-
Binding of netropsin and 4,6-diamidino-2-phenylindole to an A2T2 DNA hairpin: A comparison of biophysical techniques
Keywords: Isothermal titration calorimetry; ITC; Differential scanning calorimetry; DSC; Biosensor-surface plasmon resonance; SPR; Netropsin; DAPI; Hairpin DNA; Minor groove binding affinity; Thermodynamics
Calorimeters for biotechnology
Keywords: Calorimetry; Proteins; Lipids; Membranes; Equilibrium constant; Transitiometry; Pressure-perturbation; DSC; ITC
Imprinted soft contact lenses as norfloxacin delivery systems
Keywords: Soft contact lenses; Ocular controlled release; Molecular imprinting; ITC; Template:functional monomer ratio
Isolated Tumor Cells in Bone Marrow of Early-Stage Breast Cancer Patients
Keywords: ACOSOG; American College of Surgeons Oncology Group; BCSS; breast cancer-specific survival; CK; cytokeratin; CTC; circulating tumor cell; DDFS; distant disease-free survival; DFS; disease-free survival; DTC; disseminated tumor cell; EGF; epidermal growth
Thermodynamic Penalty Arising from Burial of a Ligand Polar Group Within a Hydrophobic Pocket of a Protein Receptor
Keywords: ITC, isothermal titration calorimetry; MUP, major urinary protein; TI-MD, thermodynamic integration-molecular dynamics; IBMP, 2-methoxy-3-isobutylpyrazineThermodynamics; ITC; binding; hydrophobicity; ligand–protein interactions
Structural characteristics correlate with immune responses induced by HIV envelope glycoprotein vaccines
Keywords: HIV; Env; gp120; gp120DV2; o-gp140; o-gp140DV2; CD4; b12; 17b; SPR; ITC; Vaccine
Thermodynamic Characterization of Binding Oxytricha nova Single Strand Telomere DNA with the Alpha Protein N-terminal Domain
Keywords: ITC, isothermal titration calorimetry; nt, nucleotides; aa, amino acids; PDB, Protein Data Banktelomere-binding protein; telomere biology; protein−single strand DNA interactions; ITC; binding energetics
Binding curves by continuous gradient flow-mix calorimetry
Keywords: 2â²CMP; cytidine-2â²-monophosphate; 3â²CMP; cytidine-3â²-monophosphate; HCl; hydrochloric acid; ITC; isothermal titration calorimetry; NaOH; sodium hydroxide; RnaseA; bovine pancreatic ribonuclease A; Flow calorimetry; Binding; Thermodynamics; Ribonuc
SMR proteins SugE and EmrE bind ligand with similar affinity and stoichiometry
Keywords: SugE; Membrane proteins; Detergents; Drug binding; Membrane mimetics; ITC; Calorimetry;
Calorimetry and mass spectrometry study of oxidized calmodulin interaction with target and differential repair by methionine sulfoxide reductases
Keywords: Calmodulin; RS20 target peptide; Oxidation; Reparation; Methionine sulfoxide reductases; CaM; calmodulin; CaMox; oxidized calmodulin; apoCaM; calcium-free calmodulin; SynCaM; hybrid calmodulin; MLCK; myosin light chain kinase; MetSO; methionine sulfoxide;
Measurements of binding thermodynamics in drug discovery
Keywords: Pharmaceutical Science; Drug Discovery; Biophysics; Thermodynamics; ligand binding; calorimetry; protein stability; isothermal titration calorimetry; ITC; differential scanning calorimetry; DSC;
Microcalorimetric studies of the effects on the interactions of human recombinant interferon-α2a
Keywords: Protein; Interferon; Stability; Aggregation; Isothermal titration calorimetry; ITC; Dilution enthalpy; Second virial coefficient; Size-exclusion chromatography; SEC;
Permeability and thermodynamics study of quaternary ammonium surfactants-phosphocholine vesicle system
Keywords: QAC; POPC; Permeability; ITC; Affinity;
Analysis of protein-surfactant interactions-a titration calorimetric and fluorescence spectroscopic investigation of interactions between Humicola insolens cutinase and an anionic surfactant
Keywords: HiC; Humicola insolens cutinase; FsC; Fusarium solani pisi cutinase; ITC; isothermal titration calorimetry; MR; molar ratio; CMC; critical micelle concentration; SDS; sodium dodecyl sulfate; 2,6-TNS; 6-(p-Toluidino)-2-naphthalenesulfonic acid; 1,8-ANS; 8-
Statistical error in isothermal titration calorimetry: Variance function estimation from generalized least squares
Keywords: ITC; Data analysis; Generalized least squares; Nonlinear least squares; Monte Carlo;
Development and initial evaluation of PEG-stabilized bilayer disks as novel model membranes
Keywords: DSPC; 1,2-distearoyl-sn-glycero-3-phosphocholine; DPC; drug partition chromatography; EPC; egg phosphatidylcholine; ITC; isothermal titration calorimetry; PEG; poly(ethyleneglycol); PEG-DSPE(5000); 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methox
Antimicrobial activity and membrane selective interactions of a synthetic lipopeptide MSI-843
Keywords: CSA; chemical shift anisotropy; CD; circular dichroism; DiPoPE; 1,2-dipalmitoleoyl-sn-glycero-3-phosphotydylethanolamine; DSC; differential scanning calorimetry; HI; normal hexagonal phase; HII; inverted hexagonal phase; ITC; isothermal titration calorime
Parvovirus B19 does not bind to membrane-associated globoside in vitro
Keywords: Parvoviridae; Parvovirus B19; Globoside; Receptor binding; Hemagglutination; SPR; Electron microscopy; ITC; Liposomes;
Conformational changes in the antibody constant domains upon hapten-binding
Keywords: Ab; antibody; mAb; monoclonal antibody; Ag; antigen; BCR; B cell receptor; BSA; bovine serum albumin; ITC; isothermal titration calorimetry; NP; (4-hydroxy-3-nitrophenyl)acetyl; NP-Cap; É-NP-aminocaproic acid; PBS; phosphate buffered saline; SpA; staphyl
Influence of the lipid composition on the membrane affinity, and the membrane-perturbing ability of cetylpyridinium chloride
Keywords: Cetylpyridinium chloride; Lipid membrane; ITC; Affinity; Permeability;
Infralimbic cortex activation increases c-fos expression in intercalated neurons of the amygdala
Keywords: picrotoxin; immediate early gene; GABA; fear; extinction; BLA; basolateral complex of the amygdala; BSA; bovine albumin serum; CEA; central amygdaloid nucleus; CeL; central nucleus lateral portion; CeM; central nucleus medial portion; CS; conditioned stim