
Coamplification at lower denaturation temperature polymerase chain reaction enables selective identification of K-Ras mutations in formalin-fixed, paraffin-embedded tumor tissues without tumor-cell enrichment
Keywords: COLD, coamplification at lower denaturation temperature; EGFR, epidermal growth factor receptor; FFPE, formalin-fixed, paraffin-embedded; PNA, peptide nucleic acidCOLD-PCR; K-Ras; Sanger sequencing