کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10795980 | 1052640 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mitochondrial Complex I decrease is responsible for bioenergetic dysfunction in K-ras transformed cells
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کلمات کلیدی
DNPtetramethylrhodamine methylestermitotracker redTMRMK-RasΔΨmOXPHOSHIF-1ROS - ROSRespiration - تنفسdinitrophenol - دینیتروفنلRh-123 - ر-123Rhodamine-123 - رودامین 123Cancer - سرطانATP synthesis - سنتز ATPhypoxia-inducible factor-1 - فاکتور القای هیپوکسی -1Oxidative phosphorylation - فسفوریلاسیون اکسیداتیوComplex I - مجتمع IMitochondria - میتوکندریاReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Many cancer cells are characterized by high rate of glycolysis and reduced rate of aerobic respiration, whose mechanism is still elusive. Here we investigate the down-regulation of oxidative phosphorylation (OXPHOS) in K-ras transformed mouse fibroblasts as compared to a control counterpart. Transcriptional analysis showed different expression levels of several OXPHOS nuclear genes in the two cell lines. In particular, during the exponential growth phase most genes encoding proteins of Complex I were expressed at lower levels in transformed cells. Consistently, a significant decrease of Complex I content was found in transformed cells. Moreover, analysis of NAD-dependent respiration and ATP synthesis indicated a strong decrease of Complex I activity in the mitochondria from neoplastic cells, that was confirmed by direct assay of the enzyme redox activity. At variance, succinate-dependent respiration and ATP synthesis were not significantly affected. Taken together, our results provide the new insight that the reduction of respiration observed in K-ras transformed cells is specifically due to a Complex I activity decrease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1797, Issue 2, February 2010, Pages 314-323
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1797, Issue 2, February 2010, Pages 314-323
نویسندگان
Alessandra Baracca, Ferdinando Chiaradonna, Gianluca Sgarbi, Giancarlo Solaini, Lilia Alberghina, Giorgio Lenaz,