کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1938023 | 1050730 | 2007 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of a novel oncogenic K-ras mutation in colon cancer Characterization of a novel oncogenic K-ras mutation in colon cancer](/preview/png/1938023.png)
Activating mutations of RAS are frequently observed in subsets of human cancers, indicating that RAS activation is involved in tumorigenesis. Here, we identified and characterized a novel G to T transversion mutation of the K-ras gene at the third position of codon 19 (TTG) which substituted phenylalanine for leucine in 3 primary colon carcinomas. Biological and biochemical activity was examined using transformed NIH3T3 cells expressing mutant or wild-type K-ras. Transformants harboring the K-ras mutation at codon 19 showed proliferative capacity under serum-starved conditions, less contact inhibition, anchorage-independent growth, tumorigenicity in nude mice and elevation of active Ras-GTP levels. These results indicated that this novel mutation possesses high oncogenic activity.
Journal: Biochemical and Biophysical Research Communications - Volume 352, Issue 3, 19 January 2007, Pages 728–732