Detection and discrimination of Mycobacterium tuberculosis complex
Keywords: Mycobacterium tuberculosis complex (MTBC); gyrB gene; SYBR® Green I; LightCycler;
مقالات ISI (ترجمه نشده)
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Évaluation de la plate-forme de PCR en temps réel LightCycler® 480 pour la mesure des charges virales CMV, EBV, HHV-6 et BKV dans le sang total
Keywords: LightCycler® 480; Diagnostic virologique moléculaire; Charge virale; Sang total; Infections virales opportunistesLightCycler® 480 system; Viral molecular diagnosis; Viral load; Whole blood specimen; Opportunistic viral infections
Analysis of activity and inhibition of oxygen-dependent enzymes by optical respirometry on the LightCycler system
Keywords: Enzymatic assays; Monoamine oxidase; Cytochrome P450; Cyclooxygenase; Optical oxygen respirometry; Oxygen-sensitive probes; Phosphorescence; LightCycler;
Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis
Keywords: Blood culture; LightCycler; multiplex; PCR; sepsis; SeptiFast
Influencing factors of dsDNA dye (high-resolution) melting curves and improved genotype call based on thermodynamic considerations
Keywords: High-resolution melting; Thermodynamics; DNA dyes; LightCycler
Asymmetric real-time PCR detection of BRCA1 5382insC mutation by melting curve analysis in the LightCycler
Keywords: BRCA1; 5382insC mutation; Real-time PCR; Melting curve analysis; LightCycler
Evaluation of a novel reverse-hybridization StripAssay for typing DNA variants useful in diagnosis of adult-type hypolactasia
Keywords: Digestive system; Genotyping; Lactose intolerance; Hypolactasia; Single-nucleotide polymorphism; LightCycler; StripAssay; Reverse-hybridization; Diagnostic techniques
Rapid detection of important human pathogenic Phleboviruses
Keywords: Bunyavirus; Phlebovirus; Taqman; Real-time PCR; Toscana virus; Rift Valley Fever virus; Lightcycler; Smartcycler;
Concordance between the deduced acetylation status generated by high-speed Real-time PCR based NAT2 genotyping of seven single nucleotide polymorphisms and human NAT2 phenotypes determined by a caffeine assay
Keywords: Acetylation status; NAT2 genotyping; NAT2 phenotyping; LightCycler
Détection de la colonisation par Streptococcus agalactiae : étude prospective comparant l'amplification génique en temps réel à un nouveau milieu chromogène Strepto B ID
Keywords: Dépistage; SGB; Streptococcus agalactiae; PCR; Gène cfb; LightCycler; Strepto B IDScreening; GBS; Streptococcus agalactiae; PCR; cfb gene; LightCycler; Strepto B ID
Pitfalls in LightCycler diagnosis of the single-nucleotide polymorphism 13.9 kb upstream of the lactase gene that is associated with adult-type hypolactasia
Keywords: Digestive system; Genotyping; Lactose intolerance; Single-nucleotide polymorphism; LightCycler; Diagnostic techniques
Rapid screening and sensitive detection of NPM1 (nucleophosmin) exon 12 mutations in acute myeloid leukaemia
Keywords: NPM1, nucleophosmin; FRET, fluorescence resonance energy transfer; MRD, minimal residual disease; AML, acute myeloid leukaemiaNPM1 mutation; Real-time PCR; MRD; LightCycler
Development of a qRT-PCR assay to determine the relative mRNA expression of two different trypsins in Atlantic cod (Gadus morhua)
Keywords: Trypsins; Serine proteases; Atlantic cod; mRNA expression; β-actin; Quantitative RT-PCR; LightCycler; FRET; Normalization
Genotyping the HFE gene by melting point analysis with the LightCycler system: Pros and cons
Keywords: HFE genotyping; LightCycler; Unusual melting profiles
Specific and quantitative detection of human polyomaviruses BKV and JCV by LightCycler® real-time PCR
Keywords: Polyomavirus; BKV; JCV; Real-time PCR; DNA load; LightCycler
Rapid genotyping of lipopolysaccharide-binding protein (LBP) C1341 → T (Leu436 → Phe) polymorphism by LightCycler real-time PCR
Keywords: Genotyping; LPS-binding protein; LightCycler; Polymorphism; Real-time PCR
Détection directe et rapide du portage de Staphylococcus aureus résistant à la méthicilline par extraction automatisée de l'acide nucléique et PCR en temps réel
Keywords: Dépistage; SARM; Staphylococcus aureus; PCR; orfX; SETS; LightCycler; MagNa PureScreening; MRSA; Staphylococcus aureus; orfX; SETS; LightCycler; MagNa Pure
Rapid, specific and quantitative assays for the detection of the endophytic bacterium Methylobacterium mesophilicum in plants
Keywords: Xylella fastidiosa; Citrus; Endophyte; Real-time quantitative PCR; LightCycler
PIK3CA mutation status in Japanese lung cancer patients
Keywords: PIK3CA; Lung cancer; Mutations; LightCycler; Genotyping
A real-time polymerase chain reaction assay for detection of Pneumocystis from bronchoalveolar lavage fluid
Keywords: LightCycler; Pneumocystis; PCR
Multiplex real-time PCR assay for simultaneous quantitation of human cytomegalovirus and herpesvirus-6 in polymorphonuclear and mononuclear cells of transplant recipients
Keywords: HCMV; HHV-6; Multiplex real-time PCR; Hybridization probes; LightCycler; Transplant recipients
Homogeneous PCR nucleobase quenching assays to detect four mutations that cause neuronal ceroid lipofuscinosis: T75P and R151X in CLN1, and IVS5-1G > C and R208X in CLN2
Keywords: CLN1; CLN2; CLN3; Neuronal ceroid lipofuscinosis; Batten disease; LightCycler; Nucleobase quenching; Mutation
Transcript quantification of Dresden G protein-coupled receptor (D-GPCR) in primary prostate cancer tissue pairs
Keywords: Expression profile; G protein-coupled receptor; LightCycler; Prostate cancer; Quantitative real-time PCR
A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens
Keywords: Neisseria gonorrhoeae; Real-time PCR; LightCycler;
Comparison of LightCycler PCR and culture for detection of group B streptococci from vaginal swabs
Keywords: Detection; group B streptococci; LightCycler; PCR; pregnant women; Streptococcus agalactiae;
Establishment of conventional and fluorescence resonance energy transfer-based real-time PCR assays for detection of pathogenic New World arenaviruses
Keywords: BSL; biosafety level; FRET; fluorescence resonance energy transfer; NP; nucleoprotein; ROX; 6-carboxy-X-rhodamine; RT-PCR; reverse transcription PCR; TCID50; 50%-tissue culture infectious dose; VHF; viral hemorrhagic fever; New World arenavirus; Viral hem
Identification of CD70 as a diagnostic biomarker for clear cell renal cell carcinoma by gene expression profiling, real-time RT-PCR and immunohistochemistry
Keywords: cDNA-arrays; LightCycler; Kidney; Microarray; Marker gene;
Quantification of C13orf19/P38IP mRNA expression by quantitative real-time PCR in patients with urological malignancies
Keywords: C13orf19; P38IP; Quantitative real-time PCR; Prostate carcinoma; Clear cell carcinoma of the kidney; amol; attomole(s) (10â18Â mol); BRCA2; breast cancer susceptibility locus 2; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HPLC; high performance liq
Rapid genotypic detection of Bacillus anthracis and the Bacillus cereus group by multiplex real-time PCR melting curve analysis
Keywords: Bacillus anthracis; Bacillus cereus group; Multiplex detection; Real-time polymerase chain reaction; LightCycler;
A rapid real-time qRT-PCR assay for ovine β-actin mRNA
Keywords: β-Actin mRNA quantification; Ovine; Real-time RT-PCR; Normalization; LightCycler; FRET;