Keywords: پروتئولیت محدود; AD; Alzheimer's disease; DTT; 1,4-dithio-d-threitol; EGTA; Ethylene glycol-bis(2-aminoethylether)-N,N,Nâ²,Nâ²-tetraacetic acid; IDP; Intrinsically disordered protein; IPTG; Isopropyl β-d-1-thiogalactopyranoside; MES; 2-(N-morpholino) ethanesulfonic aci
مقالات ISI پروتئولیت محدود (ترجمه نشده)
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Keywords: پروتئولیت محدود; DHB; 2,5-dihydroxybenzoic acid; FA; formic acid; TFA; trifluoroacetic acid; ACN; acetonitrile; MS; mass spectrometry; MALDI; matrix-assisted laser desorption/ionization; ESI; electrospray ionization; ECD; electron capture dissociation; CID; collisional in
Keywords: پروتئولیت محدود; High-throughput; Cell-free protein expression; Beads-bound protein; Limited proteolysis; Mass spectrometry; Domain;
Keywords: پروتئولیت محدود; H1, H2, H3; helices 1, 2, and 3; β1, β2; beta sheets 1 and 2; CBDPS; CyanurBiotinDiPropionylSuccinimide; PrPC; native (non-infectious) prion protein; PrPβ; β-oligomeric form of the prion protein; BS3; bis(sulfosuccinimidyl) suberate; EDC; 1-ethyl-3-[3
Structural and functional insights into the basic globulin 7S of soybean seeds by using trypsin as a molecular probe
Keywords: پروتئولیت محدود; Bg7S; Soybean globulin; Limited proteolysis; 3D structure; Insulin binding;
Reversible association of proteins into sub-visible amorphous aggregates using short solubility controlling peptide tags
Keywords: پروتئولیت محدود; BPTI; bovine pancreatic trypsin inhibitor; CD; circular dichroism; DLS; dynamic light scattering; SCP tags; solubility controlling peptide tags; SLS; static light scattering; Aggregation; Protein oligomerization; Dynamic light scattering; Limited proteoly
Auto-inhibition of adenylyl cyclase 9 (AC9) by an isoform-specific motif in the carboxyl-terminal region
Keywords: پروتئولیت محدود; Auto-inhibition; Adenylyl cyclase, cyclic AMP, myocardium; Limited proteolysis; Heart failure;
Research PaperImprovement of activity and stability of Chondroitinase ABC I by introducing an aromatic cluster at the surface of protein
Keywords: پروتئولیت محدود; Chondroitinase ABC I; Aromatic cluster; Spinal cord injury; Glial scar; Enzyme stability; Limited proteolysis;
Characterization of secondary structure and lipid binding behavior of N-terminal saposin like subdomain of human Wnt3a
Keywords: پروتئولیت محدود; Canonical Wnt signal transduction; Circular dichroism spectroscopy; Limited proteolysis; Liposomes; Saposin; Wnt3a; NT; N-terminal; CT; C-terminal; SAPLIP; saposin-like protein; SLD; saposin-like subdomain; SLD, NT-SLD(nh); N-terminal saposin-like subdoma
Multifaceted analyses of the interactions between human heart type fatty acid binding protein and its specific aptamers
Keywords: پروتئولیت محدود; Aptamer; Limited proteolysis; MALDI; Human heart type fatty acid binding protein;
Improvement of proteolytic and oxidative stability of Chondroitinase ABC I by cosolvents
Keywords: پروتئولیت محدود; cABC I, Chondroitinase ABC I; C6S, chondroitin-6-sulfate; C4S, chondroitin-4-sulfate; DS, dermatan sulfate; GAGs, galactosaminoglycans; SCI, spinal cord injuryChondroitinase ABC I; Limited proteolysis; Proteolytic stability; Oxidative stability; Cosolvent
Unfolding of chondroitinase ABC Î is dependent on thermodynamic driving force by kinetically rate constant-amplitude compensation: A stopped-flow fluorescence study
Keywords: پروتئولیت محدود; Chondroitinase ABC Î; Thermal stability; Unfolding kinetics; Stopped-flow; Conformational change; Limited proteolysis;
An integrated pipeline for sample preparation and characterization at the EMBL@PETRA3 synchrotron facilities
Keywords: پروتئولیت محدود; Protein purification; Crystallization; High-throughput; Quality control; Sample optimization; Limited proteolysis;
Proteinase K and the structure of PrPSc: The good, the bad and the ugly
Keywords: پروتئولیت محدود; Prion; PrPSc; PrPSc structure; Proteinase K; Limited proteolysis; β-Solenoid;
Multiple disulfide bridges modulate conformational stability and flexibility in hyperthermophilic archaeal purine nucleoside phosphorylase
Keywords: پروتئولیت محدود; SsMTAPII; 5â²-deoxy-5â²-methylthioadenosine phosphorylase II from Sulfolobus solfataricus; PNP; purine nucleoside phosphorylase; AdoMet; S-adenosylmethionine; MTA; 5â²-deoxy-5â²-methylthioadenosine; PDI; protein-disulfide isomerase, DSC, differential
A thermodynamic assay to test pharmacological chaperones for Fabry disease
Keywords: پروتئولیت محدود; PC; pharmacological chaperones; FD; Fabry disease; AGAL; lysosomal alpha-galactosidase; DGJ; 1-deoxy-galactonojirimycin; CD; circular dichroism; Pharmacological chaperone; Lysosomal storage disorder; Urea-induced unfolding; Limited proteolysis; Cell lysat
The interleukin-6 receptor Asp358Ala single nucleotide polymorphism rs2228145 confers increased proteolytic conversion rates by ADAM proteases
Keywords: پروتئولیت محدود; IL-6 trans-signaling; IL-6R; SNP; ADAM17; Limited proteolysis;
Expression and purification of a GRAS domain of SLR1, the rice DELLA protein
Keywords: پروتئولیت محدود; SLR1; GRAS domain; DELLA protein; GA signaling; Limited proteolysis; NMR;
Conformational changes of the bacterial type I ATP-binding cassette importer HisQMP2 at distinct steps of the catalytic cycle
Keywords: پروتئولیت محدود; ABC; ATP-binding cassette; DBD; [1,3]dioxolo[4,5-f][1,3]benzodioxole; DDM; n-dodecyl-β-D-maltopyranoside; His-tag; hexahistidine tag; IPTG; isopropyl-β-D-thio-galactopyranoside; MSP; membrane scaffold protein; MOPS; (3-(N-morpholino)-propanesulfonic aci
The E104D mutation increases the susceptibility of human triosephosphate isomerase to proteolysis. Asymmetric cleavage of the two monomers of the homodimeric enzyme
Keywords: پروتئولیت محدود; HsTIM; human triosephosphate isomerase; DTNB; 5,5â²-dithio-bis (2-nitrobenzoic acid); CAP; 3-chloroacetol phosphate; PVDF; polyvinylidene difluoride; ANS; 8-anilinonaphthalene 1-sulphonate; Triosephosphate isomerase; TIM deficiency; X-ray crystallography
Limited proteolysis regulates massive degradation of glycinin, storage 11S globulin from soybean seeds: An in vitro model
Keywords: پروتئولیت محدود; E-64, trans-epoxysuccinnyl-l-leucylamido-(4-guanidino) butane; ME, 2-mercaptoethanol; TCA, trichloroacetic acidGlycinin; Kinetics; Limited proteolysis; Seed storage globulin; Soybean
High-throughput characterization of intrinsic disorder in proteins from the Protein Structure Initiative
Keywords: پروتئولیت محدود; Intrinsically disordered proteins; Protein disorder prediction; Protein structure initiative; Limited proteolysis;
Derepression of Bacterial Transcription-Repair Coupling Factor Is Associated with a Profound Conformational Change
Keywords: پروتئولیت محدود; limited proteolysis; Mfd; protein conformational change; transcription-repair coupling factordsDNA, double-stranded DNA; NER, nucleotide excision repair; RNAP, RNA polymerase; TD1, translocation domain 1; TD2, translocation domain 2; TEC, ternary elongati
Strategies for the structural analysis of multi-protein complexes: Lessons from the 3D-Repertoire project
Keywords: پروتئولیت محدود; Protein complex validation for structural biology; Limited proteolysis; Crystallography; NMR; SAXS; KEOPS/EKC; MAK; RES complex; Dom34; Hbs1; Trm8; Trm81; Trm112;
Effects of limited proteolysis and high-pressure homogenisation on structural and functional characteristics of glycinin
Keywords: پروتئولیت محدود; Glycinin; Limited proteolysis; High-pressure homogenisation; Particle size; Surface hydrophobicity
Natural and semisynthetic azaphilones as a new scaffold for Hsp90 inhibitors
Keywords: پروتئولیت محدود; QVHBUTWFXAVRBR-QTTCSEDVSA-N; JLFSNBABVFVKGI-WLRTZDKTSA-N; HLAVRSKKJYOJBF-DMCYUOPFSA-NAzaphilones; Cancer; Antitumour; Hsp90; Chaperones; Cytotoxicity; Plasmon surface resonance; Limited proteolysis; p53; Natural products
Conformational changes in p47phox upon activation highlighted by mass spectrometry coupled to hydrogen/deuterium exchange and limited proteolysis
Keywords: پروتئولیت محدود; NADPH oxidase; p47phox; H/D exchange; Limited proteolysis; Conformational changeACN, acetonitrile; phox, phagocyte oxidase; AIR, autoinhibitory region; SH3, src homology 3; PX, Phox homology; SAXS, small-angle X-ray scattering; GST, glutathione S-transfer
Distinct recognition loop dynamics in cryptochrome-DASH and photolyase revealed by limited proteolysis
Keywords: پروتئولیت محدود; Cryptochrome; Photolyase; Photoreceptors; DNA repair; Circadian rhythm; Recognition loop; Protein dynamics; DNA base flipping; Limited proteolysis;
Structural analysis of toxic volkensin, a type 2 ribosome inactivating protein from Adenia volkensii Harm (kilyambiti plant): Molecular modeling and surface analysis by computational methods and limited proteolysis
Keywords: پروتئولیت محدود; Volkensin; Ribosome-inactivating proteins; Molecular modeling; Hydrophobic profiles; Limited proteolysis
Structure–function relationships of molluscan troponin T revealed by limited proteolysis
Keywords: پروتئولیت محدود; Invertebrate; Mollusc; Muscle; Troponin; Troponin T; Limited proteolysis
PrP N-terminal domain triggers PrPSc-like aggregation of Dpl
Keywords: پروتئولیت محدود; Prion protein; PrP N-terminal domain; Doppel; Aggregation; Limited proteolysis; Protofibrils
Mass spectrometry of full-length integral membrane proteins to define functionally relevant structural features
Keywords: پروتئولیت محدود; Mass spectrometry; Integral membrane protein; Structure–function relationship; Limited proteolysis; Protocol
Potato virus A genome-linked protein VPg is an intrinsically disordered molten globule-like protein with a hydrophobic core
Keywords: پروتئولیت محدود; Intrinsically disordered protein; Potato virus A; VPg; Limited proteolysis; CD spectroscopy; NMR
The proximal N-terminal amino acid residues are required for the coupling activity of the bovine heart mitochondrial factor B
Keywords: پروتئولیت محدود; Factor B; Limited proteolysis; Cross-link; Energy coupling; Proton leak; Oxidative phosphorylation; Mitochondria
Evidence for an extended interacting surface between β-amyloid and serum amyloid P component
Keywords: پروتئولیت محدود; Serum amyloid P component; β-Amyloid; Alzheimer's disease; Limited proteolysis; Structural study; Mass spectrometry
Translational machinery and protein folding: Evidence of conformational variants of the estrogen receptor alpha
Keywords: پروتئولیت محدود; In vivo protein folding; Protein biosynthesis; Limited proteolysis; Estrogen receptor; In vitro translation; Rabbit reticulocyte lysate; Wheat germ extract;
The universal algorithm of maturation for secretory and excretory protein precursors
Keywords: پروتئولیت محدود; Primary structure motif; Maturation process; Limited proteolysis; Step-wise degradation; Mature sequence prediction
The N-terminal domain of αB-crystallin is protected from proteolysis by bound substrate
Keywords: پروتئولیت محدود; Mass spectrometry; Limited proteolysis; Chaperone; Protein structure; α-Crystallin
Structural Domains in the Type III Restriction Endonuclease EcoP15I: Characterization by Limited Proteolysis, Mass Spectrometry and Insertional Mutagenesis
Keywords: پروتئولیت محدود; insertional mutagenesis; limited proteolysis; mass spectrometry; structural domains; Type III restriction endonuclease; EcoP15I
A photoaffinity, non-steroidal, ecdysone agonist, bisacylhydrazine compound, RH-131039: Characterization of binding and functional activity
Keywords: پروتئولیت محدود; Photoaffinity labeling; Limited proteolysis; Ecdysteroid receptor; Non-steroidal ecdysone agonist; Photoaffinity ecdysone agonist; Dipteran EcR; Lepidopteran EcR
Comparative effects of limited tryptic hydrolysis on physicochemical and structural features of seed 11S globulins
Keywords: پروتئولیت محدود; Limited proteolysis; Seed 11S globulins; Differential scanning microcalorimetry; Fluorescence; Proteolysis kinetics
Gene synthesis, expression, purification, and characterization of human Jagged-1 intracellular region
Keywords: پروتئولیت محدود; Notch signaling; Synthetic gene; Intrinsically unstructured proteins; Circular dichroism; Tryptophan fluorescence; NMR; Limited proteolysis
Location of intrinsic and inducible phenoloxidase activity in molluscan hemocyanin
Keywords: پروتئولیت محدود; Cephalopod; Enzyme induction; Gastropod; Helix pomatia; Hemocyanin; Limited proteolysis; Mollusc; Phenoloxidase activity; Sepia officinalis
Characterization of the human ortholog of Mov34 reveals eight N-terminal residues important for MPN domain stability
Keywords: پروتئولیت محدود; MPN domain protein family; Limited proteolysis; Circular dichroism; MPN domain stability
Identification of cysteinylation of a free cysteine in the Fab region of a recombinant monoclonal IgG1 antibody using Lys-C limited proteolysis coupled with LC/MS analysis
Keywords: پروتئولیت محدود; Cysteinylation; IgG; Limited proteolysis; Reversed-phase chromatography; LCMS; Mass spectrometry
Structure of a Putative Lipoate Protein Ligase from Thermoplasma acidophilum and the Mechanism of Target Selection for Post-translational Modification
Keywords: پروتئولیت محدود; pyruvate dehydrogenase complex; lipoic acid; limited proteolysis; surface loops; domainACP, acyl carrier protein; IPTG, isopropyl-β-d-thiogalactopyranoside; DTT, dithiothreitol; PDB, Protein Data Bank
Synthesis of an N-acyl sulfamate analog of luciferyl-AMP: A stable and potent inhibitor of firefly luciferase
Keywords: پروتئولیت محدود; Bioluminescence; Firefly; Luciferase; Acyl-adenylate; Adenylate-forming; Inhibitor; Sulfonamide; Sulfamate; Chymotrypsin; Limited proteolysis
Interaction with heparin protects tissue transglutaminase against inactivation by heating and by proteolysis
Keywords: پروتئولیت محدود; Transglutaminase; Heparin; Thermal inactivation; Microcalorimetry; Limited proteolysis;
Structure of a non-camelized human M12-VH domain at 1.5Â Ã
resolution
Keywords: پروتئولیت محدود; Human VH; Non-camelized; Crystal structure; Limited proteolysis;
Gir2 is an intrinsically unstructured protein that is present in Saccharomyces cerevisiae as a group of heterogeneously electrophoretic migrating forms
Keywords: پروتئولیت محدود; Gir2; Natively unfolded; Intrinsically unstructured protein; Limited proteolysis;