کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1926933 | 1536491 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Translational machinery and protein folding: Evidence of conformational variants of the estrogen receptor alpha
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Translational machinery and protein folding: Evidence of conformational variants of the estrogen receptor alpha Translational machinery and protein folding: Evidence of conformational variants of the estrogen receptor alpha](/preview/png/1926933.png)
چکیده انگلیسی
As an approach to understand how translation may affect protein folding, we analyzed structural and functional properties of the human estrogen receptor alpha synthesized by different eukaryotic translation systems. A minimum of three conformations of the receptor were detected using limited proteolysis and a sterol ligand-binding assay. The receptor in vitro translated in rabbit reticulocyte lysate was rapidly degraded by protease, produced major bands of about 34Â kDa and showed a high affinity for estradiol. In a wheat germ translation system, the receptor was more slowly digested. Two soluble co-existing conformations were evident by different degradation patterns and estradiol binding. Our data show that differences in the translation machinery may result in alternative conformations of the receptor with distinct sterol binding properties. These studies suggest that components of the cellular translation machinery itself might influence the protein folding pathways and the relative abundance of different receptor conformers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 467, Issue 2, 15 November 2007, Pages 139-143
Journal: Archives of Biochemistry and Biophysics - Volume 467, Issue 2, 15 November 2007, Pages 139-143
نویسندگان
SofÃa Horjales, Germán Cota, Mario Señorale-Pose, Carlos Rovira, Estela Román, Nora Artagaveytia, Ricardo Ehrlich, Mónica MarÃn,