Keywords: نیکل نیتریلوتوریکات اسید; 2Nal; 3-(2-naphthy)-alanine; AFWK; pheylalanine/tryptophan/lysine triple auxotrophic E. coli; DHB; 2,5-dihydroxybenzoic acid; DHF; dihydrofolate; DHFR; dihydrofolate reductase; Glu; glutamic acid; LB; Lria-Broth; Lys; lysine; MALDI-TOF MS; matrix-assisted
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Keywords: نیکل نیتریلوتوریکات اسید; Biacore; ProteOn; Octet; MX96; Antibody-antigen interactions; Optical biosensor; mAb; monoclonal antibody; SPR; surface plasmon resonance; BLI; Bio-Layer Interferometry; CFM; continuous flow microfluidics; PCSK9; proprotein convertase substilisin kexin ty
Keywords: نیکل نیتریلوتوریکات اسید; Abz; 2-aminobenzoyl; BNA; β-naphthylamide; CD; circular dichroism; DLS; dynamic light scattering; DSC; differential scanning calorimetry; DTT; dithiothreitol; DTNB; 5,5'-dithiobis(2-nitrobenzoic acid); EDTA; ethylenediaminetetraacetic acid; EDDnp; N-(2,4
Purification and characterization of the antibacterial peptidase lysostaphin from Staphylococcus simulans: Adverse influence of Zn2+ on bacteriolytic activity
Keywords: نیکل نیتریلوتوریکات اسید; Bacteriocin lysostaphin; Inhibitory metal-binding site; Staphylolytic activity; Zn2+ chelation; Zn2+-dependent endopeptidase; IMAC; immobilized metal ion affinity chromatography; MRSA; methicillin-resistant Staphylococcus aureus strain; Lss; the â¼28-kDa
Cloning, overexpression, and purification of a gene of unknown function of prophage loci from 'Candidatus Liberibacter asiaticus,' the destructive bacterial pathogen of huanglongbing disease in citrus plants
Keywords: نیکل نیتریلوتوریکات اسید; Candidatus Liberibacter asiaticus'; Huanglongbing disease; Blotchy mottle; Lime; Type-D; Prophage; CLas; 'Candidatus Liberibacter asiaticus'; E. coli; Escherichia coli; A. tumefaciens; Agrobacterium tumefaciens; A. thaliana; Arabidopsis thaliana; C.
Biophysical characterization and stabilization of detergent-solubilized lipoprotein N-acyl transferase from P. aeruginosa and E. coli
Keywords: نیکل نیتریلوتوریکات اسید; ε; molar extinction coefficient (Mâ1â¯cmâ1); EDTA; ethylenediaminetetraacetic acid; HEPES; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; HRP; horseradish peroxidase; IMAC; immobilized-metal affinity chromatography; IPTG; isopropyl β-d-1-thiog
Insights into the functional properties of the marneral oxidase CYP71A16 from Arabidopsis thaliana
Keywords: نیکل نیتریلوتوریکات اسید; P450; cytochrome P450 monooxygenase; CPR; cytochrome P450 reductase; LB; lysogeny broth (medium); TB; terrific broth (medium); IPTG; isopropyl-β-d-thiogalactopyranoside; GC/MS; gas chromatography coupled with mass spectrometry; HPLC; high performance liq
Highly stable single-strand-specific 3â²-nuclease/nucleotidase from Legionella pneumophila
Keywords: نیکل نیتریلوتوریکات اسید; 3â²-AMP; adenosine 3â²âmonophosphate; 5â²-AMP; adenosine 5â²âmonophosphate; CD; circular dichroism; DTT; 1,4âdithiotreitol; EDTA; ethylenediaminetetraacetic acid; IEF; isoelectric focusing; LCV; Legionella-containing vacuole; Lp; Legionella pneu
Expression of Cry1Ac toxin-binding region in Plutella xyllostella cadherin-like receptor and studying their interaction mode by molecular docking and site-directed mutagenesis
Keywords: نیکل نیتریلوتوریکات اسید; BSA; bovine serum albumin; cDNA; complementary DNA; ELISA; enzyme-linked immunosorbent assay; HRP; horse radish peroxidase; IPTG; isopropyl-beta-d-thiogalactopyranoside; MPBS; PBS containing skim milk; Ni-NTA; nickel-nitrilotriacetic acid; OD; optical den
Zn2+-dependent autocatalytic activity of the Bordetella pertussis CyaA-hemolysin
Keywords: نیکل نیتریلوتوریکات اسید; Autocatalysis; Bordetella pertussis; CyaA-RTX; Hydrophobic pore-forming region; Proteolytic degradation; Zn2+-metalloproteases; CyaA-Hly; hemolysin domain of CyaA toxin; Ni2+-NTA; nickel-nitrilotriacetic acid; PMSF; phenylmethylsulfonylfluoride; PNT; 1,10
Optimization of expression and purification of human mortalin (Hsp70): Folding/unfolding analysis
Keywords: نیکل نیتریلوتوریکات اسید; Hsp; heat-shock protein; CD; circular dichroism; ANS; 1-anilinonaphthalene-8-sulfonate; Ni-NTA; Nickel-nitrilotriacetic acid; Mortalin; Intrinsic fluorescence; Chaperone; CD;
Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis
Keywords: نیکل نیتریلوتوریکات اسید; Crystal structure; Toxin-antitoxin; tRNA endonuclease; Structure-function; Substrate specificity; AIDS; acquired immune deficiency syndrome; BL17U; beamline 17U; M. tb; Mycobacterium tuberculosis; PSP; PreScission protease; DTT; dithiothreitol; PMSF; phe
Acylation of the Bordetella pertussis CyaA-hemolysin: Functional implications for efficient membrane insertion and pore formation
Keywords: نیکل نیتریلوتوریکات اسید; CyaA-Hly; CyaA-hemolysin; Ch; cholesterol; DiPhyPC; 1,2-diphytanoyl-sn-glycero-3-phosphocholine; DOPC; 1,2-dioleoyl-sn-glycerol-3-phosphocholine; DOPE; 1,2-dioleoyl-sn-glycerol-3-phosphoethanolamine; NA/CyaA-Hly; non-acylated CyaA-Hly; Ni2Â +-NTA; nickel-
Research articleUse of Nicotiana tabacum transplastomic plants engineered to express a His-tagged CP47 for the isolation of functional photosystem II core complexes
Keywords: نیکل نیتریلوتوریکات اسید; Biolistic chloroplast transformation; CP47 His-tag; Photosystem II; Transplastomic tobacco plants; HEPES; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; MES; 2-(N-morpholino)ethanesulfonic acid; Ni-NTA; nickel-nitrilotriacetic acid;
Structure, biochemical and kinetic properties of recombinant Pst2p from Saccharomyces cerevisiae, a FMN-dependent NAD(P)H:quinone oxidoreductase
Keywords: نیکل نیتریلوتوریکات اسید; 1,4-BQ; 1,4-benzoquinone; CoQ1; Coenzyme Q1; DQ; duroquinone; S. cerevisiae; Saccharomyces cerevisiae; E. coli; Escherichia coli; EtOH; ethanol; HQ; hydroquinone; Lot6; low temperature-responsive 6; MQ; menadione; Ni-NTA; nickel-nitrilotriacetic acid; NQO
The unusual substrate specificity of a virulence associated serine hydrolase from the highly toxic bacterium, Francisella tularensis
Keywords: نیکل نیتریلوتوریکات اسید; DSF; differential scanning fluorimetry; FTT0941c; virulence associated serine hydrolase from Francisella tularensis; HSL; hormone sensitive lipase; KEGG; Kyoto Encyclopedia of Genes and Genomes; LB; Luria-Bertani broth; MWCO; molecular weight cut-off; Ni-
A simplified protocol for high-yield expression and purification of bacterial topoisomerase I
Keywords: نیکل نیتریلوتوریکات اسید; Type IA topoisomerase; Topoisomerase I; Streptococcus mutans; Purification; Characterization; Autoinduction; SmTopI; Streptococcus mutans topoisomerase I; VGS; Viridans Group Streptococci; VGSS; Viridans Group Streptococcal Shock Syndrome; SmTopA15b; Stre
Molecular cloning and in silico studies of physiologically significant trehalase from Drosophila melanogaster
Keywords: نیکل نیتریلوتوریکات اسید; DmTre; D. melanogaster trehalase; dNTP; deoxy nucleotide triphosphate; DTT; dithiothreitol; EtBr; ethidium bromide; h; hour; HRP; horse radish peroxide; IPTG; isopropyl-β-d-thiogalactopyranoside; LB; Luria Bertani; min; minutes; Ni-NTA; nickel-nitrilotri
G protein-membrane interactions I: Gαi1 myristoyl and palmitoyl modifications in protein-lipid interactions and its implications in membrane microdomain localization
Keywords: نیکل نیتریلوتوریکات اسید; AcNPV; Autographa californica nuclear polyhedrosis virus; AMF; 30 μM AlCl3, 50 mM MgCl2 and 10 mM NaF; Biotin-HPDP; N-[6-(biotinamido)hexyl]-3â²-(2â²-pyridyldithio) propionamide; C12E10; polyoxyethylene 10-lauryl ether; CHO; cholesterol; GPCR; G pro
Crystal structure of HlyU, the hemolysin gene transcription activator, from Vibrio cholerae N16961 and functional implications
Keywords: نیکل نیتریلوتوریکات اسید; wHTH; winged helix-turn-helix; CT; cholera toxin; IPTG; Isopropyl β-d-1-thiogalactopyranoside; Ni-NTA; nickel-nitrilotriacetic acid; PEG; polyethylene glycol; TCEP; (tris(2-carboxyethyl)phosphine); RMSD; root mean square deviation; SDM; structural di
Anti-amyloidogenic property of human gastrokine 1
Keywords: نیکل نیتریلوتوریکات اسید; Gastrokine 1; Gastric cancer; BRICHOS domain; Aβ peptide; APP; Amyloidogenesis; Aβ(1-40); amyloid-beta peptide 1-40; GKN1; gastrokine 1; flGKN1; full length GKN1; rGKN1; recombinant GKN1; proSP-C; prosurfactant protein C; Ni-NTA; nickel-nitrilotriacet
Interactions involved in grasping and locking of the inhibitory peptide IF1 by mitochondrial ATP synthase
Keywords: نیکل نیتریلوتوریکات اسید; F0F1-ATPase; ATP synthase complex (EC 3.6.3.14); F1-ATPase; isolated extrinsic part of ATP synthase; IF1; endogenous inhibitory peptide of mitochondrial ATP synthase; yIF1; IF1 from yeast; SMP; submitochondrial particle; pmf; protonmotive force; Tris; tri
Ebselen induces reactive oxygen species (ROS)-mediated cytotoxicity in Saccharomyces cerevisiae with inhibition of glutamate dehydrogenase being a target
Keywords: نیکل نیتریلوتوریکات اسید; Ebselen; ROS levels; Mitochondrial membrane potential; Glutamate dehydrogenase; Histone clipping; Yeast sporulation; CypA; Cyclophilin A; DCFH-DA; 2,7-dichlorodihydrofluorescein diacetate; FACS; florescence activated cell sorting; GDH; glutamate dehydroge
Expression and characterization of catalytic domain of Plasmodium falciparum subtilisin-like protease 3
Keywords: نیکل نیتریلوتوریکات اسید; PMSF; phenylmethylsulfonyl fluoride; Ni-NTA; nickel-nitrilotriacetic acid; MALDI-MS; matrix-assisted laser desorption ionization; MS; mass spectrometry; Plasmodium falciparum; Serine protease; Subtilisin-like protease; Erythrocytic schizogony;
The structural basis for the narrow substrate specificity of an acetyl esterase from Thermotoga maritima
Keywords: نیکل نیتریلوتوریکات اسید; 7-ACA; 7-aminocephalosporanic acid; BTB; bromothymol blue; CE7; carbohydrate esterase family 7; DSF; differential scanning fluorimetry; LB; Luria-Bertani broth; MWCO; molecular weight cut-off; Ni-NTA; nickel-nitrilotriacetic acid; NPA; 4-nitrophenol ace
The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter
Keywords: نیکل نیتریلوتوریکات اسید; His-tag; polyhistidine tag; IC50; half maximum inhibitory concentration; IPTG; isopropyl-β-d-thiogalactoside; LB; Luria-Bertani growth media; DDM; n-dodecyl-β-d-maltoside; Ni-NTA; nickel-nitrilotriacetic acid; ORF; open reading frame; SDS-PAGE; sodium d
Identification and evaluation of anti Hepatitis C virus phytochemicals from Eclipta alba
Keywords: نیکل نیتریلوتوریکات اسید; HCV; Hepatitis C virus; NS5B; non-structural protein 5B of hepatitis C virus; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; Poly (rA).(dT)18; polyriboadenylic acid annealed with (oligodeoxythymidylic acid)18; RdRp; RNA-dependant RNA
Molecular analysis of Plasmodium falciparum co-chaperone Aha1 supports its interaction with and regulation of Hsp90 in the malaria parasite
Keywords: نیکل نیتریلوتوریکات اسید; 5-FOA; 5-fluoroorotic acid; ADP; adenosine diphosphate; Aha1; Activator of Hsp90 ATPase 1; ATP; adenosine triphosphate; Cub; C-terminal half of ubiquitin; DNA; deoxyribonucleic acid; GST; glutathione S-transferase; His6; hexa-histidine; Hsp90; Heat shock
Biochemical and structural characterization of a β-1,3-1,4-glucanase from Bacillus subtilis 168
Keywords: نیکل نیتریلوتوریکات اسید; Ni-NTA; nickel-nitrilotriacetic acid; DNS; dinitrosalicylic acid; DLS; dynamic light scattering; AUC; analytical ultracentrifugation; SAXS; small angle X-ray scattering; ITFE; intrinsic tryptophan fluorescence emission; CD; circular dichroism; Bacillus su
How the N-terminal extremity of Saccharomyces cerevisiae IF1 interacts with ATP synthase: A kinetic approach
Keywords: نیکل نیتریلوتوریکات اسید; F0F1-ATPase; ATP synthase complex (EC 3.6.3.14); F1-ATPase; isolated extrinsic part of ATP synthase; IF1; endogenous inhibitory peptide of mitochondrial ATP synthase; bIF1; IF1 from bovine; yIF1; IF1 from yeast; SMP; submitochondrial particles; pmf; proto
Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies
Keywords: نیکل نیتریلوتوریکات اسید; CDR; complementarity determining region; gRNA; guide RNA; KREL; kinetoplastid RNA-editing ligase; KREPA6; kinetoplastid RNA-editing interaction protein A6; KRET2; kinetoplastid RNA-editing TUTase 2; KREX2; kinetoplastid RNA-editing 3â²Â â 5â²-exonucl
Recombinant expression and biochemical characterization of the catalytic domain of acetylcholinesterase-1 from the African malaria mosquito, Anopheles gambiae
Keywords: نیکل نیتریلوتوریکات اسید; Insecticide resistance; Mosquito; Malaria; Organophosphate; Carbamate; Cholinergic synapse; AChE; acetylcholinesterase; ATC; acetylthiocholine iodide; AβMTC; acetyl-(β-methyl)thiocholine iodide; PTC; propionylthiocholine iodide; BTC; butyrylthiocholine
Synthesis and hydrolysis of pppGpp in mycobacteria: A ligand mediated conformational switch in Rel
Keywords: نیکل نیتریلوتوریکات اسید; pppGpp; guanosine 3â²-diphosphate 5â²-triphosphate; ppGpp; guanosine 3â², 5â²-bis(diphosphate); IPTG; Isopropyl β-D-1-thiogalactopyranoside; Ni-NTA; Nickel-Nitrilotriacetic acid; HEPES; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; PEI; Poly(Et
Site-specific labeling of proteins with small molecules in live cells
Keywords: نیکل نیتریلوتوریکات اسید; ACP; acyl carrier protein; DHFR; dihydrofolate reductase; GFP; green fluorescent protein; hAGT; human O6-alkylguanine transferase; Ni-NTA; nickel-nitrilotriacetic acid; PPtase; phosphopantetheine transferase;
Characterization of recombinant human protein C inhibitor expressed in Escherichia coli
Keywords: نیکل نیتریلوتوریکات اسید; APC; activated protein C; IPTG; isopropyl-beta-d-thiogalactopyranoside; LB medium; l-broth medium; Ni-NTA; nickel-nitrilotriacetic acid; rPCI; recombinant protein C Inhibitor; P1-rPCI; R355A rPCI; P14-rPCI; T341R rPCI; Serpin; Recombinant protein C inhibi