Comparison of two poultry litter qPCR assays targeting the 16S rRNA gene of Brevibacterium sp.
Keywords: Fecal pollution; Microbial source tracking; Poultry-associated assays; qPCR;
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In-house validation of novel multiplex real-time PCR gene combination for the simultaneous detection of the main human pathogenic vibrios (Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus)
Keywords: ompW; tlh; vvhA; qPCR; Vibrio spp.; Validation;
B cell CLL/lymphoma 6 member B inhibits hepatocellular carcinoma metastases in vitro and in mice
Keywords: 5-Aza; 5-aza-2â²-deoxycytidine; 7-AAD; 7-aminoactinomycin D; BCL6B; B cell CLL/lymphoma 6 member B; cDNA; complementary DNA; FACS; fluorescence-activated cell sorting; DMEM; Dulbecco's modified Eagle medium; HCC; hepatocellular carcinoma; HTATIP2; HIV-1
Research ArticleMicroRNA-101 inhibits human hepatocellular carcinoma progression through EZH2 downregulation and increased cytostatic drug sensitivity
Keywords: EZH2; enhancer of zeste homolog 2; miR-101; microRNA-101; HCC; hepatocellular carcinoma; miRNA(s); microRNA(s); mRNA(s); messenger RNA(s); Mcl-1; myeloid cell leukemia sequence 1; siRNAs; small interfering RNAs; qPCR; quantitative real-time PCR; UTR; untr
Research ArticleDistinct miRNA signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke Opisthorchis viverrini
Keywords: CCA; cholangiocarcinoma; ICC; intrahepatic cholangiocarcinoma; HCC; hepatocellular carcinoma; FFPE; formalin fixed paraffin embedded; FC; fold change; OV; Opisthorchis viverrini; miRNA; microRNA; qPCR; quantitative reverse transcription PCR; GWU; George W
Infochemicals released by Daphnia magna fed on Microcystis aeruginosa affect mcyA gene expression
Keywords: Bloom; Cyanobacteria; Microcystins; Cladocera; qPCR; Gene expression;
A T cell gene expression panel for the diagnosis and monitoring of disease activity in patients with systemic lupus erythematosus
Keywords: SLE; systemic lupus erythematosus; RA; rheumatoid arthritis; SLEDAI; systemic lupus erythematosus disease activity index; BILAG; British Isles lupus assessment group; qPCR; real time polymerase chain reaction; CART; classification and regression trees; CM
VDR primary targets by genome-wide transcriptional profiling
Keywords: 1α,25(OH)2D3; 1α,25-dihydroxyvitamin D3; ChIP; chromatin immunoprecipitation; ChIP-Seq; ChIP-sequencing; DAVID; Database for Annotation, Visualization and Integrated Discovery; FVKM; fragments per virtual kb per million; log2fc; log2 fold change; H3K4me
Fate of enniatins and beauvericin during the malting and brewing process determined by stable isotope dilution assays
Keywords: Enniatins; Beauvericin; LC-MS/MS; Beer; qPCR;
Unexpected functional diversity in the fatty acid desaturases of the flour beetle Tribolium castaneum and identification of key residues determining activity
Keywords: Acyl-coenzyme A desaturases; Phylogenetics; Gene expression; Membrane enzymes; Unsaturated fatty acid; TcasD; Tribolium castaneum desaturase; qPCR; quantitative polymerase chain reaction; CoA; Coenzyme A; FAME; fatty acid methyl esters; DMOX 4; 4-dimethyl
Long-term ovariectomy increases BDNF gene methylation status in mouse hippocampus
Keywords: BDNF; brain-derived neurotrophic factor; COBRA; combined bisulfite restriction analysis; DG; dentate gyrus; E; 17β-estradiol; HRT; hormone replacement therapy; IOD; integrated optical density; QMSP; real-time quantitative methylation-specific PCR; PZ; po
A selection of reference genes and early-warning mRNA biomarkers for environmental monitoring using Mytilus spp. as sentinel species
Keywords: Biomonitoring; mRNA; Mytilus spp.; Pollution; qPCR; Reference genes;
Application of a novel pathogenicity marker in a multiplex real-time PCR method to assess total and pathogenic Vibrio vulnificus in food and environmental samples
Keywords: Vibrio vulnificus; vcgC; pilF; PNC; PNCC; qPCR;
Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils
Keywords: Commercial vegetable oil; CTAB; DNA detection; DNA extraction; qPCR;
Inadequate mito-biogenesis in primary dermal fibroblasts from old humans is associated with impairment of PGC1A-independent stimulation
Keywords: 12S; mitochondrial rRNA; AICAR; aminoimidazole-4-carboxamide riboside, an adenosine analogue stimulating AMPK; AMPK; catalytic α1/2-subunit of AMP dependent protein kinase; pAMPK; AMPK phosphorylated at serine 172 of the α-subunit; AU; arbitrary units;
Managing the interactions between sulfate- and perchlorate-reducing bacteria when using hydrogen-fed biofilms to treat a groundwater with a high perchlorate concentration
Keywords: Biofilm; Hydrogen; Perchlorate-reducing bacteria; Sulfate-reducing bacteria; qPCR; Pyrosequencing;
Transgenic American chestnuts show enhanced blight resistance and transmit the trait to T1 progeny
Keywords: AC; American chestnut; AMP; antimicrobial peptide; BP; base pairs; CC; Chinese chestnut; GFP; green fluorescent protein; GOI; gene of interest (here, putative resistance-enhancing gene); OD; optical density; OxO; oxalate oxidase; PDA; potato dextrose agar
Mitochondrial EF4 links respiratory dysfunction and cytoplasmic translation in Caenorhabditis elegans
Keywords: C. elegans; Caenorhabditis elegans; WT; wild-type; EF4; elongation factor 4; mtEF4; mitochondrial EF4; mtDNA; mitochondrial DNA; BP; base pairs; ETC; electron transport chain; mtEFTu, mtEFG1 and mtEFTs; mitochondrial elongation factor Tu, G1 and Ts; MMP;
Sfmbt2 10th intron-hosted miR-466(a/e)-3p are important epigenetic regulators of Nfat5 signaling, osmoregulation and urine concentration in mice
Keywords: 3â²UTR; 3â²-untranslated region; AVP; arginine vasopressin; Aqp1/2/3; aquaporin-1/2/3; Ar; aldose reductase; Cdkn1c or p57; Cyclin-dependent kinase inhibitor 1C; C2MC; Chromosome 2 miRNA cluster; Dcx; Doublecortin; ddAVP; 1-desamino-8-d-arginine vasopre
A phospholipase A2 gene is linked to Jack bean urease toxicity in the Chagas' disease vector Rhodnius prolixus
Keywords: Insect; Eicosanoid; qPCR; Transcript cloning; RNAi; Gene knockdown;
Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway
Keywords: BMP; bone morphogenetic protein; BMPR; BMP receptor; BrdU; bromodeoxyuridine; CBC; crypt-base columnar; GSK3β; glycogen synthase kinase 3β; HIEC; human intestinal epithelial crypt; LGR5; leucine-rich-repeat-containing G-protein-coupled receptor-5; OLFM4
COUP-TFII inhibits NFkappaB activation in endocrine-resistant breast cancer cells
Keywords: COUP-TFII; chicken ovalbumin upstream promoter transcription factor II; NFκB/NFkappa B; nuclear factor κB; SERM; selective estrogen receptor modulator; TAM; tamoxifen; ERα; estrogen receptor; SERD; selective estrogen receptor downregulator; 4-OHT; 4-hy
Therapeutic efficacy of the multi-epitope vaccine CTB-UE against Helicobacter pylori infection in a Mongolian gerbil model and its microRNA-155-associated immuno-protective mechanism
Keywords: Hp; Helicobacter pylori; Th1; Type 1 of helper T lymphocyte; Th2; Type 2 of helper T lymphocyte; NC; normal control group; MC; model control group; PC; positive control group; HD; high-dose group; MD; middle-dose group; LD; low-dose group; IFN-γ; interfe
Comparison of nucleic acid extraction and reverse transcription-qPCR approaches for detection of GI and GII noroviruses in drinking water
Keywords: Norovirus; qPCR; Molecular detection; Water;
In vitro evaluation of effects of gut region and fiber structure on the intestinal dominant bacterial diversity and functional bacterial species
Keywords: Bacterial diversity; Functional bacterial species; PCR-DGGE; qPCR; Kinetics;
A genome-wide survey of CD4+ lymphocyte regulatory genetic variants identifies novel asthma genes
Keywords: Asthma; CD4+ lymphocytes; regulatory variants; expression quantitative trait locus; haplotype; integrative genomics; Asthma BRIDGE; Asthma Biorepository for Integrative Genomic Exploration; CAMP; Childhood Asthma Management Program; ChIP; Chromatin immuno
Regular ArticlePtena, but not Ptenb, reduces regeneration after spinal cord injury in adult zebrafish
Keywords: CNS; central nervous system; DDC; duplication-degeneration-complementation; ISH; in situ hybridization; MO; morpholino; NMLF; nucleus of the medial longitudinal fascicle; PFA; paraformaldehyde; PBS; phosphate-buffered saline; qPCR; quantitative real-t
Validation of a Real-Time Quantitative Polymerase Chain Reaction Method for the Quantification of 3 Survivin Transcripts and Evaluation in Breast Cancer Tissues
Keywords: Apoptosis; Dual hybridization probes; Prognostic biomarkers; qPCR; Survivin splice variants;
Emodin suppresses Wnt signaling in human colorectal cancer cells SW480 and SW620
Keywords: CRC; colorectal cancer; APC; adenomatosis polyposis coli; TCF/LEF; T-cell factor / lymphoid enhancer factor; DMSO; dimethyl sulfoxide; PBS; phosphate-buffered saline; DMEM; Dulbecco׳s Modified Eagle Medium; DCFH-DA; 2â²,7â²-dichlorfluorescein-diacetate
Expression profiling of the sex-related gene Dmrt1 in adults of the Lusitanian toadfish Halobatrachus didactylus (Bloch and Schneider, 1801)
Keywords: APA; alternative polyadenylation; bp; base pairs; cDNA; DNA complementary to RNA; DEPC; diethyl dicarbonate; IHC; immunohistochemistry; ISH; in situ hybridization; mRNA; messenger RNA; ORF; open reading frame; PBST; phosphate buffered saline with Tween 20
MicroRNA-132 dysregulation in Toxoplasma gondii infection has implications for dopamine signaling pathway
Keywords: 3-MT; 3-methoxytyramine; 5-HT; serotonin; 5-HIAA; 5-hydroxyindoleacetic acid; BDNF; brain derived neurotrophic factor; COMT; catechol-O-methyltransferase; DOPAC; 3,4-dihydroxyphenylacetic acid; DRD1; dopamine receptor D1; DRD5; dopamine receptor D5; DARPP
Activation of PPARβ/δ protects pancreatic β cells from palmitate-induced apoptosis by upregulating the expression of GLP-1 receptor
Keywords: PPARβ/δ; peroxisome proliferator-activated receptor β/δ; GLP-1R; glucagon-like peptide-1 receptor; GW; GW501516; PA; palmitate; qPCR; quantitative polymerase chain reaction; SREBP-1c; sterol regulatory element binding protein-1c; GSIS; glucose-stimula
Metabolic stress-induced microRNA and mRNA expression profiles of human fibroblasts
Keywords: Human fibroblast; Stress; miRNA; mRNA; Profiling; Lipid; Galactose; qPCR
A survey of tools for the analysis of quantitative PCR (qPCR) data
Keywords: qPCR; Data analysis; MIQE; RDML; Software; Tools
RHOB influences lung adenocarcinoma metastasis and resistance in a host-sensitive manner
Keywords: GTPase; Non-cell autonomous; Microenvironment; Tumor-stroma; MMP; Chemotherapy; Radiotheraphy; Drug target; ADC; Adenocarcinoma; SQ; squamous cell carcinoma; TP; time to progression; i.c.; intracardiac; i.t.; intratibial; HMS; highly-metastatic subpopulat
Prevalence of Clostridium perfringens, Clostridium perfringens enterotoxin and dysbiosis in fecal samples of dogs with diarrhea
Keywords: RNA; 16S rRNA 16S ribosomal; AHD; acute hemorrhagic diarrhea; CPE; Clostridium perfringens enterotoxin; cpe gene; Clostridium perfringens enterotoxin gene; GI; gastrointestinal; qPCR; quantitative real-time polymerase chain reaction; RPLA; reverse passive
NqA: An R-based algorithm for the normalization and analysis of microRNA quantitative real-time polymerase chain reaction data
Keywords: Software; R package; Algorithm; Normalization; qPCR; microRNA;
Metabolomics deciphers the host resistance mechanisms in wheat cultivar Sumai-3, against trichothecene producing and non-producing isolates of Fusarium graminearum
Keywords: Deoxynivalenol; Fusarium head blight; Phenylpropanoids and flavonoids; Quantitative resistance; Sumai-3; Trichothecenes non-producing mutants (Tri5-); AUDPC; Area Under the Disease Progress Curve; C4H; Cinnamate 4-hydroxylase; CAD; Cinnamoyl alcohol dehyd
An in vitro biofilm model associated to dental implants: Structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces
Keywords: Peri-implant biofilm; Zirconium; Titanium; Hydroxyapatite; LTSEM; CLSM; qPCR
Sex differences in behavior and pro-inflammatory cytokine mRNA expression following stressor exposure and re-exposure
Keywords: BDNF; brain-derived neurotrophic factor; Ct; cycle threshold; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; IL; interleukin; IL-1R; IL-1 receptor; PFC; prefrontal cortex; qPCR; quantitative polymerase chain reaction; TNF-α; tumor necrosis factor alpha
Chibby functions in Xenopus ciliary assembly, embryonic development, and the regulation of gene expression
Keywords: RT-PCR; reverse transcription, polymerase chain reaction; MO; morpholino; AAT; acetylated α-tubulin; qPCR; quantitative reverse transcription, polymerase chain reaction.; Chibby; Cilia; Snail2; Wnt signaling; Hedgehog; Xenopus laevis; Neural crest; Neura
Molecular cloning, expression profiles and promoter analysis of insulin-like growth factor binding protein-4 (IGFBP-4) in Japanese flounder (Paralichthys olivaceus)
Keywords: dph; day post hatching; qPCR; quantitative real-time PCR; Paralichthys olivaceus; Insulin-like growth factor binding protein-4; cDNA cloning; mRNA expression; Promoter analysis;
Denitrification in restored and unrestored Danish streams
Keywords: Denitrification; Nitrogen; Organic matter; PCR-DGGE; qPCR; Stream restoration
Seasonal variations of unicellular diazotroph groups A and B, and Trichodesmium in the northern South China Sea and neighboring upstream Kuroshio Current
Keywords: nifH gene; qPCR; Nitrogen fixation; Typhoon; Internal wave
Role of white adipose lipolysis in the development of NASH induced by methionine- and choline-deficient diet
Keywords: Acaca; acetyl-coenzyme A carboxylase alpha; Acly; ATP citrate lyase; Adrb; β-adrenergic receptor; ALT; alanine aminotransferase; Apob; apolipoprotein B; ATGL; adipose triglyceride lipase; CD; choline-deficient diet; Ces3; carboxylesterase 3; Dgat; diacyl
TLR3 mediated innate immune response in mice brain following infection with Chikungunya virus
Keywords: Chikungunya; Neuropathogenesis; TLR; Innate immune response; CPE; Cytopathic effect; M.O.I; multiplicity of infection; TPB; tryptose phosphate buffer; MEM; minimal essential media; PFU; plaque forming unit; RIPA; radioimmunoprecipitation assay; TLR; Toll
A systematic comparison of four bioaerosol generators: Affect on culturability and cell membrane integrity when aerosolizing Escherichia coli bacteria
Keywords: Bioaerosol; Aerosolization; Aerosol generator; Cell damage; qPCR; Microorganism stress
Quantitative expression profiling in mouse spinal cord reveals changing relationships among channel and receptor mRNA levels across postnatal maturation
Keywords: ACHE; acetylcholinesterase; ANOVA; analysis of variance; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; qPCR; quantitative polymerase chain reaction; spinal cord maturation; gene expression; real-time PCR; ion channels; neurotransmitter receptor;
Protease Nexin-1 affects the migration and invasion of C6 glioma cells through the regulation of urokinase Plasminogen Activator and Matrix Metalloproteinase-9/2
Keywords: PN-1; Protease Nexin-1; MMP-9 and MMP-2; Matrix Metalloproteinase-9 and -2; uPA; urokinase Plasminogen Activator; shRNA; short hairpin RNA; ECM; extracellular matrix; tPA; tissue plasminogen activator; LRP; low-density lipoprotein receptor-related protein
Effect of submarine groundwater discharge on bacterial indicators and swimmer health at Avalon Beach, CA, USA
Keywords: Indicator organisms; Water quality; Marine water; qPCR; Gastrointestinal illness