کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8338347 | 1541005 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Long-term ovariectomy increases BDNF gene methylation status in mouse hippocampus
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کلمات کلیدی
qPCRHRTWHIMSqMSPCOBRAIOD17β-estradiol - 17β استرادیولBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز Estrogen - استروژنcombined bisulfite restriction analysis - تجزیه و تحلیل محدود بیسولفیتintegrated optical density - تراکم نوری یکپارچهreal-time quantitative PCR - زمان واقعی PCR کمیSynaptophysin - سیناپتوفیزینSyn - سینتdentate gyrus - شکنج دندانه دارBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزDNA methylation - متیلاسیون DNAhormone replacement therapy - هورمون جایگزین درمانHippocampus - هیپوکامپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Estradiol (E) has been suggested to have a neuroprotective effect in young animals but has neutral or harmful effects when it is administered to aged animals. In the present study, we determined whether the post-ovariectomy (post-OVX) timeframe elapsed before the initiation of chronic E treatment is critical for the estrogenic induction of neurotrophins (brain-derived neurotrophic factor, BDNF, and synaptophysin, SYN) in the rodent hippocampus. Adult mice were OVX and, a short period (short-term E (STE) animals) or a long period (long-term E (LTE) animals) after the OVX, were daily treated with E. Control animals were treated with sesame oil (short-term control (STC) and long-term control (LTC) animals). Protein expression was determined using an immunohistochemical approach. Transcriptional activity in the hippocampus of individual BDNF promoters was assessed by real-time quantitative RT-PCR, and the methylation levels of regulatory regions were analyzed by methylation-specific PCR and combined bisulfite restriction analysis. STE animals showed increased BDNF and SYN protein expression and a higher activity of BDNF II, IV, and V promoters. In contrast, LTE animals did not show E induction of neurotrophins. In these animals, the methylation levels of regulatory sequences of the BDNF were higher than in the STE animals in a CpG island of promoter V and in the CRE regulatory site located in promoter IV. With this experiment, we determined that a prolonged period of hypoestrogenicity disrupts the E-induction of neurotrophins, and we postulated that DNA methylation is one of the epigenetic mechanisms that could explain the E-insensitivity of the BDNF after a long period post-OVX.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 144, Part B, October 2014, Pages 243-252
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 144, Part B, October 2014, Pages 243-252
نویسندگان
Guillermo S. Moreno-Piovano, Jorgelina Varayoud, Enrique H. Luque, Jorge G. Ramos,