DNA-duplex linker for AFM-SELEX of DNA aptamer against human serum albumin
Keywords: SELEX; Atomic force microscope; DNA aptamer; Human serum albumin;
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An ssDNA library immobilized SELEX technique for selection of an aptamer against ractopamine
Keywords: Aptamer; Ractopamine; SELEX; Library immobilization; Graphene oxide;
Development of a ssDNA aptamer for detection of residual benzylpenicillin
Keywords: Antibiotics; BenzylPenicillin; Aptamer; SELEX; rGO-SELEX; SELEX; systematic evolution of ligands by exponential enrichment; rGO; reduced graphene oxide; Kd; dissociation constant; LOD; limit of detection; MRL; maximum residue limit;
A novel method for detection of H9N2 influenza viruses by an aptamer-real time-PCR
Keywords: Influenza; Aptamer; H9N2; I-PCR; SELEX; Diagnostic;
Design, isolation and evaluation of the binding efficiency of a DNA aptamer against interleukin 2 receptor alpha, in vitro
Keywords: Aptamer; Interleukin-2 receptor alpha; CD25; Jurkat cells; SELEX;
Selection, characterization, and application of DNA aptamers for detection of Mycobacterium tuberculosis secreted protein MPT64
Keywords: Mycobacterium tuberculosis; Diagnostics; MPT64; Aptamer; SELEX;
Integrated analysis of RNA-binding protein complexes using in vitro selection and high-throughput sequencing and sequence specificity landscapes (SEQRS)
Keywords: RNA; Sequencing; SELEX; Pumilio; NOS; Combinatorial control;
Electrochemical aptasensor for human osteopontin detection using a DNA aptamer selected by SELEX
Keywords: Osteopontin; SELEX; DNA aptamers; Screen-printed gold electrode; Electrochemical aptasensor;
ABCs of DNA aptamer and related assay development
Keywords: Aptamers; SELEX; Aptamer library; Colorimetric assay; Aptamer Linked Immunosorbent Assay (ALISA); FRET; ssDNA generation; Post-SELEX optimization; Translation of aptamers;
An aptamer-based effective method for highly sensitive detection of chloramphenicol residues in animal-sourced food using real-time fluorescent quantitative PCR
Keywords: B&W; washing and binding; CAP; chloramphenicol; Ct; cycle threshold; dsDNA; double stranded DNA; ELISA; enzyme linked immunosorbent assay; EU; European Union; FF; florfenicol; FRET; fluorescence resonance energy transfer; GC; gas chromatography; LC; liqui
Structural optimization of an aptamer generated from Ligand-Guided Selection (LIGS) resulted in high affinity variant toward mIgM expressed on Burkitt's lymphoma cell lines
Keywords: Aptamer; SELEX; B-Cell Receptor; truncation; LIGS;
Detection of Aβ oligomers based on magnetic-field-assisted separation of aptamer-functionalized Fe3O4 magnetic nanoparticles and BaYF5:Yb,Er nanoparticles as upconversion fluorescence labels
Keywords: Aβ; amyloid-β peptide; Aβo; Aβ oligomers; AD; Alzheimer's disease; MNPs; magnetic nanoparticles; UCNPs; upconversion nanoparticles; NPs; nanoparticles; CSF; cerebrospinal fluid; SELEX; Systematic Evolution of Ligands by EXponential enrichment; UV-vi
DNA aptamer probes for detection of estrogen receptor α positive carcinomas
Keywords: BSA; bovine serum albumin; DAB; 3,3' diaminobenzidine; ER α; estrogen receptor alpha; FAM; 6-fluorescein; HRP; Horseradish peroxidase; ITC; isothermal calorimetry; NC; nitro cellulose membrane; PBS; phosphate buffered saline; SELEX; Systematic Evoluti
Application of aptamers in detection and chromatographic purification of antibiotics in different matrices
Keywords: Aptamer; Antibiotic; SELEX; Sensor; Purification; Detection; Environment; Food; Drinking water; Biological sample;
In vitro evolution of coenzyme-independent variants from the glmS ribozyme structural scaffold
Keywords: APM; N-acryloylaminophenylmercuric acetate; dNTPs; deoxyribonucleotide triphosphates; DTT; dithiothreitol; GlcN6P; glucosamine-6-phosphate; glmSWT; wild-type glmS ribozyme; 6SGMP; 6-thioguanosine monophosphate; glmSAAA; triple adenosine-mutant glmS ribozy
Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonist
Keywords: Aptamer; SELEX; Staphylococcal enterotoxin A; Superantigen
Identification of RNA aptamers with riboswitching properties
Keywords: Aptamer; Riboswitch; Genetic engineering; SELEX; Screening system
An in vitro selection for small molecule induced switching RNA molecules
Keywords: SELEX; Stand displacement; In vitro selection; Riboswitch;
Selection of a high-affinity and in vivo bioactive ssDNA aptamer against angiotensin II peptide
Keywords: Angiotensin II; Aptamer; SELEX; Serum electrolytes; VSMCs; Urine volume
Analyzing HT-SELEX data with the Galaxy Project tools – A web based bioinformatics platform for biomedical research
Keywords: SELEX, Systematic Evolution of Ligands by EXponential enrichment; HTS, high-throughput sequencingAptamer; SELEX; Bioinformatics; Galaxy; High-throughput sequencing (HTS); Next-generation sequencing (NGS)
Integrated microfluidic device using a single universal aptamer to detect multiple types of influenza viruses
Keywords: Ca++; Calcium ion; ddH2O; Double-distilled water; FAM; Carboxyfluorescein; HA; Hemagglutination; InfA; Influenza A virus; InfB; Influenza B virus; K+; Potassium ion; LOD; Limit of detection; Mg++; Magnesium ion; Na+; Sodium ion; NC; Negative control; PBS;
The efficient cell-SELEX strategy, Icell-SELEX, using isogenic cell lines for selection and counter-selection to generate RNA aptamers to cell surface proteins
Keywords: RNA aptamer; Isogenic cell-SELEX; RNA silencing; Cell surface proteins; Integrin alpha V; Icell-SELEX; isogenic cell-SELEX; SELEX; systematic evolution of ligands by exponential enrichment; ITGAV; integrin alpha V; miRNA; microRNA; O.E.-cells; overexpress
Aptamers: The “evolution” of SELEX
Keywords: SELEX; Aptamers;
Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolates
Keywords: O-Phenylenediamidin (pubchem CID:11990); Tween 20 (pubchem CID: 443314)Aptamer; Acinetobacter baumannii; SELEX; ELASA; Flow cytometry
Generation and characterization of novel DNA aptamers against coat protein of grouper nervous necrosis virus (GNNV) with antiviral activities and delivery potential in grouper cells
Keywords: Aptamer; SELEX; Grouper nervous necrosis virus; Coat protein; Antiviral activity;
The in vitro selection world
Keywords: In vitro selection; SELEX; RNA ligands; Aptamer; Next generation sequencing; Phage display; In vivo; In silico;
Aptamer-based ‘point-of-care testing’
Keywords: Aptamer; SELEX; Sensor; Antibody; Portable
In vitro selection of RNA aptamers against CA125 tumor marker in ovarian cancer and its study by optical biosensing
Keywords: Aptamers; SELEX; CA125; Surface Plasmon Resonance
AFBI assay - Aptamer Fluorescence Binding and Internalization assay for cultured adherent cells
Keywords: SELEX; Systematic Evolution of Ligands by EXponential enrichment; AFBI assay; Aptamer Fluorescence Binding and Internalization assay; qPCR; quantitative PCR; Aptamer; SELEX; Cell-SELEX; Fluorescence; Microplate reader;
Screening and development of DNA aptamers as capture probes for colorimetric detection of patulin
Keywords: Patulin; Aptamer; SELEX; Graphene oxide; Enzyme-chromogenic;
Selection and characterization of DNA aptamers for the development of light-up biosensor to detect Cd(II)
Keywords: Aptamer; SELEX; Biosensor; Cadmium;
Aptamers as smart ligands for nano-carriers targeting
Keywords: Aptamer; Targeting; Nano-carriers; Cancer therapy; Affinity ligand; 225Ac; Actinium isotope 225; Bcl-xl; B-cell lymphoma-extra large transmembrane protein; CD; Cluster of differentiation; CNTs; Carbon nanotubes; CTLA 4; cytotoxic T-lymphocyte-associated p
Isolation and characterization of 2â²-F-RNA aptamers against whole HIV-1 subtype C envelope pseudovirus
Keywords: HIV-1; Enveloped pseudotyped virus; Entry inhibition; SELEX; Aptamers;
Selection of DNA aptamers against Human Cardiac Troponin I for colorimetric sensor based dot blot application
Keywords: DNA aptamer; Selex; Human Cardiac Troponin I; Gold nanoparticle; Dot blot assay;
Computer-aided design of aptamers for cytochrome p450
Keywords: VEGF; vascular endothelial growth factor; SELEX; Systematic Evolution of Ligands by EXponential enrichment; CYP51; cytochrome p450 51A1, lanosterol 14α-demethylase; MD; molecular dynamics; PME; particle mesh Ewald; MM-PBSA; Molecular Mechanics Poissonâ
DNA aptamers selection and characterization for development of label-free impedimetric aptasensor for neurotoxin anatoxin-a
Keywords: Anatoxin-a; SELEX; Aptamer; Aptasensor; Label-free; Impedance;
Amino acid residues Leu135 and Tyr236 are required for RNA binding activity of CFIm25 in Entamoeba histolytica
Keywords: Polyadenylation; Cleavage factor Im; Mutagenesis; RNA binding; Entamoeba histolytica; CPSF; Cleavage and Polyadenylation Specificity Factor; CFIm and CFIIm; Cleavage Factor I and II; CstF; Cleavage Stimulatory Factor; IPTG; isopropyl beta-d-thiogalactopyr
Nanoparticle-conjugated aptamer targeting hnRNP A2/B1 can recognize multiple tumor cells and inhibit their proliferation
Keywords: Tumor cells; SELEX; Aptamer; Heterogeneous nuclear ribonucleoprotein A2/B1; Nanoparticles
Generation and characterization of nucleic acid aptamers targeting the capsid P domain of a human norovirus GII.4 strain
Keywords: SELEX; Aptamer; Norovirus; Molecular detection; Viral diagnostics;
Imidazole-free purification of His3-tagged recombinant proteins using ssDNA aptamer-based affinity chromatography
Keywords: DNA aptamers; SELEX; Histidine tag; IMAC; Imidazole;
Rapid evolution of in vivo-selected sequences and structures replacing 20% of a subviral RNA
Keywords: Turnip crinkle virus; Satellite RNA; Viral RNA evolution; SELEX;
Current approaches in SELEX: An update to aptamer selection technology
Keywords: SELEX; Aptamers; PCR; Oligonucleotide library; In silico selection; Post-SELEX;
Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1
Keywords: Aptamer; Staphylococcus aureus enterotoxin C1; SELEX; Graphene oxide; Food;
Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase
Keywords: Acetohydroxyacid synthase; Tuberculosis; SELEX; DNA aptamer; Inhibitor;
Recognition of candidate aptamer sequences for human hepatocellular carcinoma in SELEX screening using structure–activity relationships
Keywords: Aptamer; Human hepatocellular carcinoma; Pattern recognition; Structure–activity relationship; SELEX; Support vector classification
Selection of DNA aptamers against epidermal growth factor receptor with high affinity and specificity
Keywords: EGFR; epidermal growth factor receptor; SELEX; systematic evolution of ligands by exponential enrichment; RPTK; receptor protein tyrosine kinase; NSCLC; non-small-cell lung carcinoma; TKI; tyrosine kinase inhibitors; VEGF; vascular endothelial growth fact
In vitro selection of RNA aptamers that selectively bind danofloxacin
Keywords: Danofloxacin; RNA aptamer; SELEX
An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening
Keywords: Dual aptamers; Bovine viral diarrhea virus (BVDV); SELEX; SPR; Sandwich
Sequence-constructive SELEX: A new strategy for screening DNA aptamer binding to Globo H
Keywords: Globo H; SELEX; DNA aptamer;
Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEX
Keywords: M. tuberculosis; SELEX; Aptamer; EsxG;