کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5140728 | 1495766 | 2017 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of Aβ oligomers based on magnetic-field-assisted separation of aptamer-functionalized Fe3O4 magnetic nanoparticles and BaYF5:Yb,Er nanoparticles as upconversion fluorescence labels
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کلمات کلیدی
TEOStetraethyl orthosilicateNIRSELEXAβMNPs - MNP هاNPs - NP هاUCNPs - UCNP هاUV–Vis - UV-VisAminopropyltrimethoxysilane - آمینوپروپیلل تریموتوکسیلانAptamer - آپتامرهاEDTA - اتیلن دی آمین تترا استیک اسید Ultraviolet–visible - اشعه ماوراء بنفش قابل مشاهده استAβ oligomers - الیگومرهای AβTem - این استAlzheimer's disease - بیماری آلزایمرsystematic evolution of ligands by exponential enrichment - تکامل سیستماتیک لیگاند ها با غنی سازی نمایشیAβo - دو تاFTIR - طیف سنج مادون قرمزFourier transform infrared spectroscopy - طیف سنجی مادون قرمز تبدیل فوریه یا طیف سنجی FTIRCerebrospinal fluid - مایع مغزی نخاعیCSF - مایع مغزی نخاعیAPTES - مناسبTransmission electron microscopy - میکروسکوپ الکترونی عبوریNanoparticles - نانوذراتUpconversion nanoparticles - نانوذرات تبدیل تبدیلMagnetic nanoparticles - نانوذرات مغناطیسیFe3O4 magnetic nanoparticles - نانوذرات مغناطیسی Fe3O4XRD - پراش اشعه ایکسamyloid-β peptide - پپتید آمیلیید ب
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We developed a sensitive and stable bioassay for detection of Aβ oligomers (Aβo), a potentially promising candidate biomarker for Alzheimer's disease (AD) diagnosis. We first prepared the amino-functionalized Fe3O4 magnetic nanoparticles (MNPs) and BaYF5:Yb,Er upconversion nanoparticles (UCNPs), which were conjugated with avidin by a classical glutaraldehyde method. Then the biotinylated Aβo aptamer DNA1 was immobilized on avidin-conjugated MNPs, and its complementary DNA2 was linked to avidin-conjugated UCNPs, all through the biotin-avidin affinity reaction, in order to prepare the DNA1-functionalized MNPs and DNA2-functionalized UCNPs. Then the aptamer DNA1 hybridized with its part complementary DNA2 to form the duplex structure on the surface of the MNPs/UCNPs nanoprobe, therefore were assembled onto the surface of MNPs giving a background upconversion fluorescent signal. In the presence of Aβo, the aptamer DNA1 preferentially bound with Aβo and caused the dissociation of some complementary DNA2, liberating some UCNP-labeled complementary DNA2 and leading to a decreased upconversion fluorescent signal on the surface of MNPs. Then after magnetic separation with an external magnet, highly specific and sensitive detection of Aβo was achieved in connection with measurement of the upconversion fluorescent signal of the unreleased UCNPs on the surface of MNPs. The decreased fluorescence intensity of UCNPs was related to the concentration of Aβo in the range of 0.2-15 nM with a detection limit of 36 pM. The developed method then was successfully applied to measure Aβo in artificial cerebrospinal fluid. Benefiting from the magnetic separation and concentration effect of MNPs, the high sensitivity of UCNPs, as well as the selectivity and stability of the aptamer, the present strategy offered valuable information related to early diagnosis of AD process.232
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 170, 1 August 2017, Pages 350-357
Journal: Talanta - Volume 170, 1 August 2017, Pages 350-357
نویسندگان
Ling-Feng Jiang, Bo-Cheng Chen, Ben Chen, Xue-Jian Li, Hai-Lin Liao, Hong-Miao Huang, Zhan-Jing Guo, Wen-Yan Zhang, Lin Wu,