Keywords: Sf9; UPR; ER stress; CaMKII; Calcium signaling;
مقالات ISI (ترجمه نشده)
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Keywords: Insect cell culture in reagent bottlesInsect cell; Baculovirus; BV; Sf9; Sf21; High-throughput; Fermentation vessel; Reagent bottle; High volume culture; Expression
Keywords: CC50; concentration of the compound required to reduce cell viability by 50%; CSF; classical swine fever; CSFV; classical swine fever virus; Ct; cycle threshold; ER; endoplasmic reticulum; GTs; glycosyltransferases; IC50; concentration of the compound req
Keywords: BV; Baculovirus, also used as emptyBac (no transgene insert); sialBac; (BV containing the human sialin sequence); cysBac; (BV containing the human CTNS sequence); BVES; Baculovirus Expression System; Sf9; Spodptera frugiperda cell line 9 (from ovarian tis
Keywords: A re-usable wave bioreactor for protein production in insect cellsInsect cells; Sf9; Hi5; Upscale; Bioreactor; Wave bag; Protein production
Keywords: Transfection in suspension; Baculovirus; Insect cell; Sf9; Lipofectin; Polyethyleneimine (PEI); High-throughput
Single-cell cloning enables the selection of more productive Drosophila melanogaster S2 cells for recombinant protein expression
Keywords: AMP; antimicrobial peptide/protein; BR021; Harmonia axyridis antimicrobial peptide BR021; BSA; bovine serum albumin; DMSO; dimethyl sulfoxide; EGFP; enhanced green fluorescent protein; FACS; fluorescence activated cell sorting; FBS; fetal bovine serum; Gm
γ-Taxilin temporally regulates centrosome disjunction in a Nek2A-dependent manner
Keywords: C-Nap1; centrosomal never in mitosis A-related kinase-associated protein 1; Nek2; never in mitosis A-related kinase 2; APC/C; anaphase promoting complex/cyclosome; ER; endoplasmic reticulum; PCR; polymerase chain reaction; siRNA; small interfering RNA; PB
Testosterone metabolism of equine single CYPs of the 3A subfamily compared to the human CYP3A4
Keywords: POR; NADPH P450 oxidoreductase; CYPs; cytochrome P450 enzymes; CYB5; cytochrome b5; Sf9; insect cells of Spodoptera frugiperda; HPLC; high-pressure liquid chromatography; NADPH; nicotinamide adenine dinucleotide phosphate; NDSB; non-detergent sulfobetaine
Regulation of BmBDV NS1 by phosphorylation: Impact of mutagenesis at consensus phosphorylation sites on ATPase activity and cytopathic effects
Keywords: Bombyx mori bidensovirus; Parvovirus nonstructural protein 1; Baculovirus expression vector; Sf9; Enhanced green fluorescent protein
The key molecular events during Macrobrachium rosenbergii nodavirus (MrNV) infection and replication in Sf9 insect cells
Keywords: MrNV; Virus replication; Sf9; Caveolin-mediated endocytosis pathway;
The histamine H4-receptor and the central and peripheral nervous system: A critical analysis of the literature
Keywords: Histamine H4 receptor; Nervous system; Neuron; Microglia; Antibody specificity; Publication bias; A 943931; H4R antagonist (4-((3R)-3-amino-pyrrolidin-1-yl)-6,7-dihydro-5H-benzo[6,7]cyclohepta[1,2-d]pyrimidin-2-ylamine); ACh; acetylcholine; AKT; protein k
PDE7A1 hydrolyzes cCMP
Keywords: A549; human lung carcinoma cell line; AMP; adenosine 5â²-monophosphate; cAMP; adenosine 3â²,5â²-cyclic monophosphate; B103; rat neuroblastoma cells; CMP; cytidine 5â²-monophosphate; cCMP; cytidine 3â²,5â²-cyclic monophosphate; CyaA; Bordetella pertu
Recombinant PNPLA3 protein shows triglyceride hydrolase activity and its I148M mutation results in loss of function
Keywords: PNPLA3; patatin-like phospholipase domain containing 3; ADPN; adiponutrin; Sf9; Spodoptera frugiperda 9; LPAAT; lysophosphatidic acid acyl transferase; YPDS; Yeast Extract Peptone Dextrose plus Sorbitol; BMGY; buffered glycerol-complex medium; BMMY; buffe
Synthesis and analysis of potential α1,3-fucosyltransferase inhibitors
Keywords: ADP; adenosine diphosphate; dGDP; deoxyguanosine diphosphate; (h)FucT; (human) fucosyltransferase; IDP; inosine diphosphate; GDP; guanosine diphosphate; GMP; guanosine monophosphate; GT; glycosyltransferase; GTP; guanosine triphosphate; HF9; soluble recom
Cell proliferation and modulation of interaction of estrogen receptors with coregulators induced by ERα and ERβ agonists
Keywords: ATP; adenosine triphosphate; BrdU; 5-bromo-2â²-deoxyuridine; BSA; bovine serum albumin; CCD; charge coupled device; CDTA; 1,2-diaminocyclo-hexanetetraacetic acid; CHO; Chinese hamster ovary; DCC-FCS; dextran-coated charcoal-treated fetal calf serum; DMEM
Human α-N-acetylglucosaminidase: cDNA cryptic site removal and native secretion signal addition significantly enhance enzyme expression and secretion
Keywords: MPS IIIB; mucopolysaccharidosis III subtype B or Sanfilippo syndrome type B; Naglu; human α-N-acetylglucosaminidase; Sf9; Spodoptera frugiperda; modTat; synthetically optimized protein transduction domain also known as PTD4; RT-PCR; reverse transcription
Modification of p115RhoGEF Ser330 regulates its RhoGEF activity
Keywords: ATRA; All-trans retinoic acid; DH; Dbl homology; DMEM; Dulbecco's modified Eagle's medium; FBS; Fetal bovine serum; GAP; GTPase activating protein; HPLC-MS/MS; High pressure/tandem mass spectrometry; LARG; Leukemia-associated RhoGEF; LC/MS/MS; Liquid ch
Anti-influenza A virus activity of uridine derivatives of 2-deoxy sugars
Keywords: CC50; concentration of the compound required to reduce cell viability by 50%; CPE; cytopathic effect; CSFV; classical swine fever virus; Ct; cycle threshold; ER; endoplasmic reticulum; HA; hemagglutinin; IC50; concentration of the compound required to red
Cloning and characterization of a Dim1-like mitosis gene of Spodoptera frugiperda cells (Sf9) induced by Autographa californica multiple nucleopolyhedrovirus
Keywords: Dim1-like gene; Sf9; Cell proliferation; Virus–host interaction; AcMNPV
Expression, purification, electron microscopy, N-glycosylation mutagenesis and molecular modeling of human P2X4 and Dictyostelium discoideum P2XA
Keywords: β-OG; n-octyl-β-d-glucoside; C12E8; octaethylene glycol monododecyl ether; DDM; n-dodecyl-β-d-maltoside; FC-12; n-dodecylphosphocholine; LDAO; n-dodecyl-N,N-dimethylamine-N-oxide; PBS; phosphate-buffered saline; SDS; sodium dodecyl sulfate; Sf9 cells;
GPCR stabilization using the bicelle-like architecture of mixed sterol-detergent micelles
Keywords: DPH; 1,6-diphenyl-1,3,5-hexatriene; Compound-24; 1-benzyl-N-{3-[spiroisobenzofuran-1(3H),40-piperidin-1-yl]propyl} pyrrolidine-2-carboxamide; β1AR; β1-adrenergic receptor; β2AR; β2-adrenergic receptor; CCM; cholesterol consensus motif; CHS; cholestery
Preliminary studies on induction of apoptosis by abamectin in Spodoptera frugiperda (Sf9) cell line
Keywords: Abamectin; Sf9; Apoptosis; Mitochondrion; Cytochrome-c; Caspase-3
Cleavage-resistant fusion proteins of the M2 muscarinic receptor and Gαi1. Homotropic and heterotropic effects in the binding of ligands
Keywords: CHO; Chinese hamster ovary; DEAE; diethylaminoethyl; DTT; dithiothreitol; GMP-PNP; guanylylimidodiphosphate; GPCR; G protein-coupled receptor; HA; influenza hemagglutinin; IB; immunoblot; IP; immunoprecipitate; MOI; multiplicity of infection; NMS; N-methy
The synthesis and characterization of cellular membrane affinity chromatography columns for the study of human multidrug resistant proteins MRP1, MRP2 and human breast cancer resistant protein BCRP using membranes obtained from Spodoptera frugiperda (Sf9)
Keywords: Sf9; Affinity chromatography; Drug discovery; Receptor screening; ABC transporters; P-glycoprotein; MRP1; MRP2; BCRP; Membrane receptors
Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occâ baculovirus occlusion and larval per os infectivity
Keywords: Polyhedrin; Stable cell lines; Sf9; GFP; Baculovirus;
Deletion of the AcMNPV core gene ac109 results in budded virions that are non-infectious
Keywords: Baculovirus; AcMNPV; Sf9; ac109; bv/odv-ec43; Core gene; Budded virus
Mitochondrial regulation of insect cell apoptosis: Evidence for permeability transition pore-independent cytochrome-c release in the Lepidopteran Sf9 cells
Keywords: Apoptosis; Ca2+; Cytochrome-c; Mitochondrial membrane potential; Sf9; Permeability transition
An efficient method to express GPI-anchor proteins in insect cells
Keywords: Baculovirus; Insect cells; Sf9; High Five; Glycosylphosphatidylinositols; N-glycosylation; Toxoplasma gondii; N-acetylglucosaminyl phosphatidylinositol de-N-acetylase; PIGL; SAG1
Functional analysis of a Drosophila melanogaster olfactory receptor expressed in Sf9 cells
Keywords: Olfactory receptor; Calcium imaging; Olfaction; Sf9; Odorant; Or22a
N-Glycosylation sites of plant purple acid phosphatases important for protein expression and secretion in insect cells
Keywords: Purple acid phosphatase; PAP; Glycosylation; Site-directed mutagenesis; Protein secretion; Sf9
A universal transgene silencing approach in baculovirus–insect cell system
Keywords: RNAi; siRNA; Baculovirus; Bicistronic; Sf9; LqqIT2; Fusion; EGFP
Recovery of functional peptide transporter PepT1 in budded baculovirus fraction
Keywords: Transporter; PepT1; Sf9; Baculovirus; Budded virus; Uptake;
Suppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein
Keywords: RNAi; shRNA; PRRSV; Baculovirus; VSV G; Marc145; SF9
Native tubulin-folding cofactor E purified from baculovirus-infected Sf9 cells dissociates tubulin dimers
Keywords: TBCE purification; Baculovirus expression; Sf9; Tubulin dissociation;
Phosphorylation at the hydrophobic site of protein kinase C Apl II is increased during intermediate term facilitation
Keywords: Aplysia; learning and memory; phosphoinositide dependent kinase I; persistent protein kinase; rapamycin; autophosphorylation; PDBu; phorbol dibutyrate; PDK-1; phosphoinositide-dependent kinase I; PI-3 kinase; phosphoinositide-3 kinase; PKC; protein kinase
Cell-binding properties of the envelope proteins of porcine endogenous retroviruses
Keywords: Porcine endogenous retroviruses; Receptors; Xenotransplantation; DMEM; Dulbecco's modified Eagle's medium; FCS; fetal calf serum; FeLV; feline leukemia virus; GaLV; gibbon ape leukemia virus; HEK; human embryonic kidney; MAb; monoclonal antibody; MLV; mur
Regulatory mechanisms underlying pheromone biosynthesis activating neuropeptide (PBAN)-induced internalization of the Bombyx mori PBAN receptor
Keywords: PBAN receptor; PBAN; Bombyx mori; GPCR; Confocal microscopy; EGFP; Receptor-mediated endocytosis; Sf9; Agonist-induced internalization;
CYP2K6 from zebrafish (Danio rerio): Cloning, mapping, developmental/tissue expression, and aflatoxin B1 activation by baculovirus expressed enzyme
Keywords: AFB1; Aflatoxin B1; BSA; Bovine serum albumin; Bv-2K6; Baculovirus/insect cell expressed recombinant CYP2K6; CYP; Cytochrome P450; EST; Expressed sequence tag; GSH; Glutathione; HPLC; High pressure liquid chromatography; LC-MS; Liquid chromatography-mass
Cloning and characterization of the G protein βγ subunits from Trichoplusia ni (High Five⢠cells)
Keywords: G protein; Gbeta/gamma (Gβγ); Gbeta (Gβ); Ggamma (Gγ); Trichoplusia ni; High fiveâ¢; Heterotrimeric; Baculovirus; Spodoptera frugiperda; Sf9;