کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8451634 | 1547696 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
γ-Taxilin temporally regulates centrosome disjunction in a Nek2A-dependent manner
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کلمات کلیدی
Spodoptera frugiperda 9PBSC-Nap1nek2ATF4anaphase promoting complex/cyclosomeGFPSf9GSTPAGEAPC/C - APC / CBSA - BSACEP - MOBILERNA interference - RNA تداخل کنندهSmall interfering RNA - RNA تداخل کوچکRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsiRNA - siRNAbovine serum albumin - آلبومین سرم گاوpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدreverse transcription - رونویسی معکوسCentriole - سانترالendoplasmic reticulum - شبکه آندوپلاسمی Phosphate buffered saline - فسفات بافر شورactivating transcription factor 4 - فعال کردن عامل رونویسی 4polymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازgreen fluorescent protein - پروتئین فلورسنت سبزCell cycle - چرخه سلولیglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: γ-Taxilin temporally regulates centrosome disjunction in a Nek2A-dependent manner γ-Taxilin temporally regulates centrosome disjunction in a Nek2A-dependent manner](/preview/png/8451634.png)
چکیده انگلیسی
Never in mitosis A-related kinase 2A (Nek2A), a centrosomal serine/threonine kinase, is involved in mitotic progression by regulating the centrosome cycle. Particularly, Nek2A is necessary for dissolution of the intercentriole linkage between the duplicated centrosomes prior to mitosis. Nek2A activity roughly parallels its cell cycle-dependent expression levels, but the precise mechanism regulating its activity remains unclear. In this study, we found that γ-taxilin co-localized with Nek2A at the centrosome during interphase and interacted with Nek2A in yeast two-hybrid and pull-down assays and that γ-taxilin regulated centrosome disjunction in a Nek2A-dependent manner. γ-Taxilin depletion increased the number of cells with striking splitting of centrosomes. The precocious splitting of centrosomes induced by γ-taxilin depletion was attenuated by Nek2A depletion, suggesting that γ-taxilin depletion induces the Nek2A-mediated dissolution of the intercentriole linkage between the duplicated centrosomes nevertheless mitosis does not yet begin. Taken together with the result that γ-taxilin protein expression levels were decreased at the onset of mitosis, we propose that γ-taxilin participates in Nek2A-mediated centrosome disjunction as a negative regulator through its interaction with Nek2A.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 362, Issue 2, 15 January 2018, Pages 412-423
Journal: Experimental Cell Research - Volume 362, Issue 2, 15 January 2018, Pages 412-423
نویسندگان
Tomohiko Makiyama, Satoru Higashi, Hiroshi Sakane, Satoru Nogami, Hiromichi Shirataki,