|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|2129994||1086516||2016||7 صفحه PDF||سفارش دهید||دانلود رایگان|
• Podocyte injury induced by sublytic C5b-9 attack is the main feature of membranous nephropathy.
• Autophagy contributes critically to the structural and functional integrity of differentiated podocytes.
• Sublytic complement attack can enhance podocyte autophagy in vitro.
• Inhibition of autophagy enhances sC5b-9-induced podocyte injury while promotion of autophagy mitigates injury.
• Autophagy can protect podocytes from sC5b-9-induced injury in vitro.
Podocyte injury induced by sublytic complement attack is the main feature of membranous nephropathy (MN). This study aimed at investigating the impact of sublytic complement attack-related autophagy on podocyte injury in vitro. Here, we show that sublytic complement attack enhances MPC5 podocyte autophagy in vitro. Inhibition of autophagy by treatment with 3-methyladenine (3-MA) significantly increased sublytic complement attack-induced changes in the injury-related morphology, stress fiber, and podocyte apoptosis, but decreased the survival and adhesion of MPC5 podocytes. In contrast, promotion of autophagy by treatment with rapamycin mitigated sublytic complement attack-induced changes in the injury-related morphology, stress fiber, and podocyte apoptosis, but increased the survival and adhesion of MPC5 podocytes. These data suggest that autophagy may protect podocytes from sublytic complement attack-induced injury in vitro.
Journal: Experimental Cell Research - Volume 341, Issue 2, 15 February 2016, Pages 132–138