کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6492283 | 43523 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occâ baculovirus occlusion and larval per os infectivity
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occâ baculovirus occlusion and larval per os infectivity Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occâ baculovirus occlusion and larval per os infectivity](/preview/png/6492283.png)
چکیده انگلیسی
Transfection of Sf9 cells with plasmid carrying polyhedrin gene and stable cell lines established by selection with blasticidin showed polyhedrin expression and, moreover, crystalline polyhedron-like structures were visualized by optic microscopy. Oral infectivity was demonstrated by fluorescence detection in Rachiplusia nu larvae infected with occluded AcGFPpolhâ baculovirus obtained using the system presented here.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 145, Issue 2, 15 January 2010, Pages 199-205
Journal: Journal of Biotechnology - Volume 145, Issue 2, 15 January 2010, Pages 199-205
نویسندگان
MarÃa Gabriela López, Victoria Alfonso, Elisa Carrillo, Oscar Taboga,