دانلود مقالات ISI درباره mutagenesis مواجه با سایت + ترجمه فارسی

Serine/threonine/tyrosine protein kinase from Arabidopsis thaliana is dependent on serine residues for its activity

Keywords: mutagenesis مواجه با سایت; Dual-specificity protein kinase in plants; Site-directed mutagenesis; Kinase assays

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2007 سفارش ترجمه

The conserved glycine/alanine residue of the active-site loop containing the putative acetylCoA-binding motif is essential for the overall structural integrity of Mesorhizobium loti arylamine N-acetyltransferase 1

Keywords: mutagenesis مواجه با سایت; NAT; arylamine N-acetyltransferase; RMSD; root mean square deviation; RMSF; root mean square fluctuation; MLNAT1; Mesorhizobium loti NAT1; Arylamine N-acetyltransferase; P-loop; Active-site; Steric hindrance; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2007 سفارش ترجمه

Basic fibroblast growth factor: Lysine 134 is essential for its neuroprotective activity

Keywords: mutagenesis مواجه با سایت; bFGF; Growth factor; Neuroprotection; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2007 سفارش ترجمه

Ammonia channeling in bacterial glucosamine-6-phosphate synthase (Glms): Molecular dynamics simulations and kinetic studies of protein mutants

Keywords: mutagenesis مواجه با سایت; Glms/Gfat, bacterial/human glucosamine-6P synthase; SMD, steered molecular dynamicsAmidotransferases; Glms; Ammonia transfer; Molecular dynamics simulations; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2007 سفارش ترجمه

Improving the activity and stability of thermolysin by site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; FAGLA, N-[3-(2-furyl)acryloyl]-glycyl-l-leucine amide; npr, neutral protease gene; ZDFM, N-carbobenzoxy-l-aspartyl-l-phenylalanine methyl esterCatalytic activity; Metalloproteinase; Site-directed mutagenesis; Thermal stability; Thermolysin

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2007 سفارش ترجمه

N-Glycosylation sites of plant purple acid phosphatases important for protein expression and secretion in insect cells

Keywords: mutagenesis مواجه با سایت; Purple acid phosphatase; PAP; Glycosylation; Site-directed mutagenesis; Protein secretion; Sf9

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2007 سفارش ترجمه

Re-engineering cytochrome P450 2B11dH for enhanced metabolism of several substrates including the anti-cancer prodrugs cyclophosphamide and ifosfamide

Keywords: mutagenesis مواجه با سایت; Cytochrome P450; P450 2B11; P450 engineering; Structure–function relationships; Site-directed mutagenesis; Enzyme catalysis

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2007 سفارش ترجمه

Rat NAD+-dependent 3α-hydroxysteroid dehydrogenase (AKR1C17): A member of the aldo-keto reductase family highly expressed in kidney cytosol

Keywords: mutagenesis مواجه با سایت; Aldo-keto reductase superfamily; AKR1C17; 3α-Hydroxysteroid dehydrogenase; Bile acid; Ketamine; Inhibitor sensitivity; Organic anion; Coenzyme specificity; Kidney-specific protein; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2007 سفارش ترجمه

Rational design of novel mutants of fungal 17β-hydroxysteroid dehydrogenase

Keywords: mutagenesis مواجه با سایت; Biotransformation of steroids; 17β-Hydroxysteroid dehydrogenase; Fungi; Cochliobolus lunatus; Site-directed mutagenesis; Enzyme engineering

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2007 سفارش ترجمه

Properties of a chimeric glucose dehydrogenase improved by site directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Glucose dehydrogenase; Escherichia coli; S. marcescens GPS-5; Mineral phosphate solubilization; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2007 سفارش ترجمه

His164 regulates accessibility to the active site in fungal 17β-hydroxysteroid dehydrogenase

Keywords: mutagenesis مواجه با سایت; 17β-Hydroxysteroid dehydrogenase; Short-chain dehydrogenase/reductase; Site-directed mutagenesis; Kinetic characterization; Thermodynamic analysis

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Involvement of Gly 311 residue on substrate discrimination, pH and temperature dependency of recombinant Staphylococcus xylosus lipase: A study with emulsified substrate

Keywords: mutagenesis مواجه با سایت; Staphylococcus xylosus lipase; Site-directed mutagenesis; Expression; Purification; Steric hindrance; Substrate discrimination; Catalytic efficiency; Phospholipase activity;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Investigating the role of active site residues of Rhodotorula gracilisd-amino acid oxidase on its substrate specificity

Keywords: mutagenesis مواجه با سایت; d-amino acid oxidase (DAAO, EC 1.4.3.3)Flavoprotein; Site-directed mutagenesis; Reaction mechanism; Substrate recognitionRgDAAO, Rhodotorula gracilisd-amino acid oxidase; pkDAAO, pig kidney d-amino acid oxidase; EFlox, oxidized enzyme; EFlseq, enzyme semi

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Glycine oxidase from Bacillus subtilis: Role of Histidine 244 and Methionine 261

Keywords: mutagenesis مواجه با سایت; Flavoprotein; Site-directed mutagenesis; Substrate recognition; Thiamine biosynthesis;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Point mutations at the local anesthetic receptor site modulate the state-dependent block of rat Nav1.4 sodium channels by pyrazoline-type insecticides

Keywords: mutagenesis مواجه با سایت; Indoxacarb; RH 3421; DCJW; Nav1.4; Oocyte; Insecticide; Local anesthetic receptor; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Local Anesthetics Have Different Mechanisms and Sites of Action at Recombinant 5-HT3 Receptors

Keywords: mutagenesis مواجه با سایت; Recombinant; Subunit; Site-directed mutagenesis; Oocyte; Electrophysiology;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

The N-terminal region of the starch-branching enzyme from Phaseolus vulgaris L. is essential for optimal catalysis and structural stability

Keywords: mutagenesis مواجه با سایت; SBE; starch-branching enzyme; GBE; glycogen-branching enzyme; DP; degree of polymerization; HPAEC-PAD; high-performance anion exchange chromatography-pulsed amperometric detector; PvSBE; kidney bean (Phaseolus vulgaris L.) SBE; (1Na/2Nb)-II; N-termina

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers

Keywords: mutagenesis مواجه با سایت; Cutinase; Biocatalysis; Polyester; Polyamide 6,6; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

The effects of reaction conditions on the production of γ-cyclodextrin from tapioca starch by using a novel recombinant engineered CGTase

Keywords: mutagenesis مواجه با سایت; Bacillus sp. G1; Cyclodextrin glucanotransferase; Gamma-cyclodextrin; Pullulanase; Site-directed mutagenesis; Tapioca starch

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Engineered disulfide bonds increase active-site local stability and reduce catalytic activity of a cold-adapted alkaline phosphatase

Keywords: mutagenesis مواجه با سایت; Cold-adaptation; Site-directed mutagenesis; Catalytic efficiency; Cysteine; Disulfide bond

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2007 سفارش ترجمه

Constraints imposed by transmembrane domains affect enzymatic activity of membrane-associated human CD39/NTPDase1 mutants

Keywords: mutagenesis مواجه با سایت; E-NTPDase; Endothelial ecto-ATPDase; Ecto-nucleotidase; CD39; Site-directed mutagenesis; Apyrase conserved regions; Multimerization; Oligomer; Thrombosis

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Characterization and comparison of the intronic promoter of murine osteoclastic protein-tyrosine phosphatase, PTP-oc, with the human PTP-oc promoter

Keywords: mutagenesis مواجه با سایت; Promoter; Protein-tyrosine phosphatase; Osteoclasts; Site-directed mutagenesis; Deletion analysis; Chromatin immunoprecipitation

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Structural and mutational analyses reveal the functional role of active-site Lys-154 and Asp-173 of Salmonella typhimurium AphA protein

Keywords: mutagenesis مواجه با سایت; Site-directed mutagenesis; Crystal structure; Salmonella typhimurium AphA; Metallo phosphatase; HAD phosphotransferase;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Functional mapping and single chain construction of the anti-cytokeratin 8 monoclonal antibody TS1

Keywords: mutagenesis مواجه با سایت; scFv construction; Functional mapping; Interacting interface; Site-directed mutagenesis; Hotspot

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Phenylalanine90 and phenylalanine93 are crucial amino acids within the estrogen binding site of the human UDP-glucuronosyltransferase 1A10

Keywords: mutagenesis مواجه با سایت; UGTs; UGT1A10; Estrogens; Binding site; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Characterization of rat and mouse NAD+-dependent 3α/17β/20α-hydroxysteroid dehydrogenases and identification of substrate specificity determinants by site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Aldo-keto reductase superfamily; 3α-Hydroxysteroid dehydrogenase; 3α/17β/20α-Hydroxysteroid dehydrogenase; Steroid specificity; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2007 سفارش ترجمه

The Grb2/PLD2 Interaction Is Essential for Lipase Activity, Intracellular Localization and Signaling in Response to EGF

Keywords: mutagenesis مواجه با سایت; shRNA, short hairpin RNA; EGFR, epidermal growth factor receptor; PLD, phospholipase D; PH, pleckstrin homology; PX, phox; WT, wild-typePLD2; Grb2; shRNA; co-localization; site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2007 سفارش ترجمه

Role of the amino acid invariants in the active site of MurG as evaluated by site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Glycosyltransferase; Invariant amino acids; MurG; Peptidoglycan; Site-directed mutagenesis; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; GlcNAc; N-acetylglucosamine; IPTG; isopropyl-β-d-galactopyranoside; MurNAc; N-acetylmuramoyl; PM

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2007 سفارش ترجمه

Hybrid homology modeling and mutational analysis of cytochrome P450C24A1 (CYP24A1) of the Vitamin D pathway: Insights into substrate specificity and membrane bound structure–function

Keywords: mutagenesis مواجه با سایت; Vitamin D; Calcitriol; 25-hydroxyvitamin D3 24R-hydroxylase; 1,25-dihydroxyvitamin D3; Vitamin D inactivation; Cytochrome P450; CYPs; P450C24A1; CYP24A1; Site-directed mutagenesis; Homology modeling; Affinity labeling; Monotopic membrane protein

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2007 سفارش ترجمه

Modification of the active site of Mycobacterium tuberculosis KatG after disruption of the Met–Tyr–Trp cross-linked adduct

Keywords: mutagenesis مواجه با سایت; Mycobacterium tuberculosis; Catalase-peroxidase; Resonance Raman; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2007 سفارش ترجمه

Identification of ginsenoside interaction sites in 5-HT3A receptors

Keywords: mutagenesis مواجه با سایت; Ginsenosides; 5-HT3A receptor; Site-directed mutagenesis; Xenopus oocytesRg3, ginsenoside Rg3; 5-HT, 5-hydroxytryptamine (serotonin); 5-HT3A receptor, serotonin receptor 3A subunit; MES, 4-Morpholineethanesulfonic acid; NMDG, N-methyl-d-glucamine; TM2, tr

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2007 سفارش ترجمه

Differential functional phenotypes of two primary HIV-1 strains resulting from homologous point mutations in the LLP domains of the envelope gp41 intracytoplasmic domain

Keywords: mutagenesis مواجه با سایت; Site-directed mutagenesis; Cell fusion; HIV envelope protein gp41; HIV; Viral infectivity; Virus replication; HIV envelope incorporation and biosynthesis

دانلود رایگان متن کامل مقاله ISI 15 صفحه سال انتشار : 2007 سفارش ترجمه

Hierarchical Disabled-1 Tyrosine Phosphorylation in Src family Kinase Activation and Neurite Formation

Keywords: mutagenesis مواجه با سایت; SFK, Src family kinase; pSFK, phospho-SFK; VLDLR, very low density lipoprotein receptor; Dab1, Disabled-1; GFP, green fluorescent protein; RT-PCR, reverse-transcription PCRretina; alternative splicing; tyrosine phosphorylation; site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 16 صفحه سال انتشار : 2007 سفارش ترجمه

Properties of the [NiFe]-hydrogenase maturation protein HypD

Keywords: mutagenesis مواجه با سایت; [NiFe]-hydrogenase; Maturation; HypD; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2006 سفارش ترجمه

Infrared spectroscopic study on Ca2+ binding to Akazara scallop troponin C in comparison with peptide analogues of its Ca2+-binding Site IV

Keywords: mutagenesis مواجه با سایت; Infrared spectroscopy; Structure-function correlation; Site-directed mutagenesis; Synthetic peptide analogue;

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2006 سفارش ترجمه

Molecular determinants of dihydrouridine synthase activity

Keywords: mutagenesis مواجه با سایت; DUS, dihydrouridine synthase; DHODH, dihydroorotate dehydrogenase; DHPDH, dihydropyrimidine dehydrogenase; FMN, flavin mononucleotidetRNA; tRNA modification; Dihydrouridine; Dihydrouridine synthase; YjbN; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2006 سفارش ترجمه

The cytochrome P450 gene family CYP157 does not contain EXXR in the K-helix reducing the absolute conserved P450 residues to a single cysteine

Keywords: mutagenesis مواجه با سایت; CYP, cytochrome P450; PCR, polymerase chain reactionCytochrome P450; Site-directed mutagenesis; Protein folding; Streptomyces

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2006 سفارش ترجمه

The lid is a structural and functional determinant of lipase activity and selectivity

Keywords: mutagenesis مواجه با سایت; Candida rugosa lipase isoform 1; Bacillus subtilis lipase A; Pseudomonas fragi lipase; Specificity; Temperature stability; Enantioselectivity; Site-directed mutagenesis; Domain swapping

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2006 سفارش ترجمه

Protein engineering of class-A non-specific acid phosphatase (PhoN) of Salmonella typhimurium: Modulation of the pH-activity profile

Keywords: mutagenesis مواجه با سایت; Site-directed mutagenesis; PhoN protein of Salmonella typhimurium; Class-A bacterial non-specific acid phosphatase; Protein engineering; pH-optimum; Bioremediation

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2006 سفارش ترجمه

Contribution of W229 to the transglycosylation activity of 4-α-glucanotransferase from Pyrococcus furiosus

Keywords: mutagenesis مواجه با سایت; 4-α-glucanotransferase (4-α-GTase); Site-directed mutagenesis; Transglycosylation activity; Hydrolysis activity; Hydrophobicity

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

An over expression and high efficient mutation system of a cobalt-containing nitrile hydratase

Keywords: mutagenesis مواجه با سایت; Nitrile hydratase; Recombinant E. coli; Site-directed mutagenesis; Thermo-stability; Product tolerance

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Carbohydrate-binding site of a novel mannose-specific lectin from fugu (Takifugu rubripes) skin mucus

Keywords: mutagenesis مواجه با سایت; Lectin; Fish skin mucus; Fugu; Monocotyledonous plant; Carbohydrate-binding site; d-mannose; Recombinant protein; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Decrease of current responses at human recombinant P2X3 receptors after substitution by Asp of Ser/Thr residues in protein kinase C phosphorylation sites of their ecto-domains

Keywords: mutagenesis مواجه با سایت; Nucleotides; Ecto-protein kinase C; P2X3 receptor-phosphorylation; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Drug susceptibilities of yeast cells are affected when expressing mutant Candida albicans drug resistance protein

Keywords: mutagenesis مواجه با سایت; Candida drug resistance protein; Site-directed mutagenesis; Drug susceptibility

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Targeted mutagenesis of a fatty acid Δ6-desaturase from Mucor rouxii: Role of amino acid residues adjacent to histidine-rich motif II

Keywords: mutagenesis مواجه با سایت; Δ6-Desaturase; Mucor rouxii; γ-Linolenic acid; Histidine-rich motif; Site-directed mutagenesis; Substrate recognition

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Effects of mutations at conserved TM II residues on ligand binding and activation of mouse 5-HT6 receptor

Keywords: mutagenesis مواجه با سایت; Site-directed mutagenesis; Mouse 5-HT6 receptor

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Identification of the zinc binding ligands and the catalytic residue in human aspartoacylase, an enzyme involved in Canavan disease

Keywords: mutagenesis مواجه با سایت; CD, Canavan disease; hASP, human aspartoacylase; CP, carboxypeptidase; SGD, succinylglutamate desuccinylase from Chromobacterium violaceum; NAA, N–acetylaspartateAspartoacylase; Metalloenzyme; Molecular modelling; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Reaction mechanism of bovine heart cytochrome c oxidase

Keywords: mutagenesis مواجه با سایت; Cytochrome c oxidase; Heme protein; Membrane protein; Proton pump; O2 reduction; Electron transfer; X-ray crystallography; Resonance Raman spectroscopy; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2006 سفارش ترجمه

Site-directed mutagenesis of the Thauera aromatica strain T1 tutE tutFDGH gene cluster

Keywords: mutagenesis مواجه با سایت; Thauera aromatica; Anaerobic toluene metabolism; Benzylsuccinate synthase; Site-directed mutagenesis; ATP binding protein; Glycine-free radical activation;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2006 سفارش ترجمه

On the effects of site-specific mutations on activity and expression of the Streptomyces PMF phospholipase D

Keywords: mutagenesis مواجه با سایت; Phospholipase D; Streptomyces PMF; Site-directed mutagenesis; HKD Domain; Heterologous expression

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2006 سفارش ترجمه