The N-terminal region of the starch-branching enzyme from Phaseolus vulgaris L. is essential for optimal catalysis and structural stability

The present study provides new insight into the relationships between the N-terminal region and enzyme activity of the starch-branching enzyme through the enzymatic analyses of the chimeric, truncated, and site-directed mutant enzymes. We also identified the crucial residues in the N-terminal region for optimal catalysis and structural stability.