دانلود مقالات ISI درباره mutagenesis مواجه با سایت + ترجمه فارسی

Structural and functional characterisation of the chlorite dismutase from the nitrite-oxidizing bacterium “Candidatus Nitrospira defluvii”: Identification of a catalytically important amino acid residue

Keywords: mutagenesis مواجه با سایت; Chlorite dismutase; Candidatus Nitrospira defluvii; 3D-structure; Enzyme kinetics; Structure-based alignment; Site-directed mutagenesis; Catalytic mechanism; Heme proteins; (Per)chlorate bioremediation;

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2010 سفارش ترجمه

Role of tyrosine 33 residue for the stabilization of the tetrameric structure of human cytidine deaminase

Keywords: mutagenesis مواجه با سایت; Cytidine deaminase; Site-directed mutagenesis; Molecular modeling; Circular dichroism;

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2010 سفارش ترجمه

Unraveling the mechanism of action of a new type III secretion system effector AexU from Aeromonas hydrophila

Keywords: mutagenesis مواجه با سایت; Aeromonas hydrophila; T3SS; AexU; Site-directed mutagenesis; ADP-ribosyltransferase activity; GAP activity; HeLa Tet-Off cells; Apoptosis; Septicemic mouse model of infection

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2010 سفارش ترجمه

Selection of single chain variable fragment (scFv) antibodies from a hyperimmunized phage display library for the detection of the antibiotic monensin

Keywords: mutagenesis مواجه با سایت; Monensin; Antibiotics; Single chain variable fragment; Site-directed mutagenesis; Fluorescence polarization

دانلود رایگان متن کامل مقاله ISI 16 صفحه سال انتشار : 2010 سفارش ترجمه

Structure and Mechanism of the Magnesium-Independent Aromatic Prenyltransferase CloQ from the Clorobiocin Biosynthetic Pathway

Keywords: mutagenesis مواجه با سایت; crystal structure; site-directed mutagenesis; PT fold; antibiotic; StreptomycesDMATS, dimethylallyl tryptophan synthase; PT fold, prenyltransferase fold; 4HPP, 4-hydroxyphenylpyruvate; DMAPP, dimethylallyl pyrophosphate; GPP, geranyl pyrophosphate; PDB, P

دانلود رایگان متن کامل مقاله ISI 16 صفحه سال انتشار : 2010 سفارش ترجمه

Rational design of Bacillus stearothermophilus US100 l-arabinose isomerase: Potential applications for d-tagatose production

Keywords: mutagenesis مواجه با سایت; l-Arabinose isomerase; d-Tagatose; Site-directed mutagenesis; Thermoactivity; Acidotolerance

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2009 سفارش ترجمه

Mutations that affect coenzyme binding and dimer formation of fungal 17β-hydroxysteroid dehydrogenase

Keywords: mutagenesis مواجه با سایت; 17β-Hydroxysteroid dehydrogenase; Site-directed mutagenesis; Coenzyme binding; Dimerization

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2009 سفارش ترجمه

Spare interactions of highly potent [Arg14,Lys15]nociceptin for cooperative induction of ORL1 receptor activation

Keywords: mutagenesis مواجه با سایت; EL2; The second extracellular loop; GPCR; G protein-coupled receptor; ORL1; Opioid receptor-like receptor 1; TM5; The fifth transmembrane domain; Binding site; Nociceptin; Receptor activation; Site-directed mutagenesis; Synergistic effect;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Kinetic analysis of site-directed mutants of methionine synthase from Candida albicans

Keywords: mutagenesis مواجه با سایت; Methionine synthase; Cobalamin independent; Site-directed mutagenesis; Candida albicans; Molecular model; Fusion protein; Active site mapping; Inhibitor design

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Functional assignment of Glu386 and Arg388 in the active site of l-galactono-γ-lactone dehydrogenase

Keywords: mutagenesis مواجه با سایت; AldO, alditol oxidase; CO, cholesterol oxidase; GALDH, l-galactono-γ-lactone dehydrogenase; VAO, vanillyl-alcohol oxidasel-Galactono-1,4-lactone dehydrogenase (EC 1.3.2.3); Vitamin C; Flavoprotein; VAO family; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Enzymatic characterization and mutational studies of TruD – the fifth family of pseudouridine synthases

Keywords: mutagenesis مواجه با سایت; RNA modification; Pseudouridine; Pseudouridine synthase; Enzyme mechanism; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Charge storage investigation in self-assembled monolayer of redox-active recombinant azurin

Keywords: mutagenesis مواجه با سایت; 85.65.+h; 82.37.RsSelf-assembled monolayer; Bioelectronic device; Recombinant azurin; Site-directed mutagenesis; Scanning tunneling microscopy; Nanobiochip

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Multi-bit biomemory consisting of recombinant protein variants, azurin

Keywords: mutagenesis مواجه با سایت; Azurin; Site-directed mutagenesis; Nano-biochip; Atomic force microscopy; Open circuit potential amperometry; Multi-bit information storage

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

The site for the allosteric activator GTP of Escherichia coli UMP kinase

Keywords: mutagenesis مواجه با سایت; UMPK; UMP kinase; EcUMPK; UMP kinase from Escherichia coli; BaUMPK; UMP kinase from Bacillus anthracis; GMPPNP; 5′-guanylyl imidodiphosphate; Uridylate kinase; Pyrimidine biosynthesis; Feed-back inhibition; Site-directed mutagenesis; Allosteric regulati

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Biomemory device composed of mutant azurin thin films modified by site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Bioelectronic device; Site-directed mutagenesis; Surface plasmon resonance; Scanning tunneling microscopy; Raman spectroscopy; Cyclic voltammetry; Nanobiochip

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2009 سفارش ترجمه

An active-site mutation enhances the catalytic activity of the yeast Cryptococcus humicolad-aspartate oxidase

Keywords: mutagenesis مواجه با سایت; d-Aspartate oxidase; Cryptococcus humicola; FAD enzyme; Site-directed mutagenesis; Yeast

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2009 سفارش ترجمه

The effect of substitution of Phe181 and Phe182 with Ala on activity, substrate specificity and stabilization of substrate at the active site of Bacillus thermocatenulatus lipase

Keywords: mutagenesis مواجه با سایت; Bacillus thermocatenulatus; Lipase; Lid; Steric hindrance; van der Waals; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2009 سفارش ترجمه

Structural and mechanistic roles of three consecutive Pro residues of porcine NADH-cytochrome b5 reductase for the binding of β-NADH

Keywords: mutagenesis مواجه با سایت; NADH binding; Electron transfer; Site-directed mutagenesis; Pro residue; FAD

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Molecular and biochemical evolution of maize terpene synthase 10, an enzyme of indirect defense

Keywords: mutagenesis مواجه با سایت; Zea mays; Teosinte; Sesquiterpene synthase; Terpene synthase reaction mechanism; β-Farnesene; α-Bergamotene; Molecular modeling; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Insights into the catalytic properties of bamboo vacuolar invertase through mutational analysis of active site residues

Keywords: mutagenesis مواجه با سایت; Bambusa oldhamii; Poaceae; Bamboo; Homology modeling; Site-directed mutagenesis; Vacuolar invertase;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Residues Leu52 and Leu134 are important for the structural integrity of a nucleotide exchange factor GrpE from Bacillus licheniformis

Keywords: mutagenesis مواجه با سایت; GrpE; Bacillus licheniformis; Site-directed mutagenesis; ATPase activity

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Engineering the activity of thermophilic xylose isomerase by site-directed mutation at subunit interfaces

Keywords: mutagenesis مواجه با سایت; Xylose isomerase; Thermus thermophilus; Site-directed mutagenesis; Enzyme activity; Subunit interfaces

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Engineering of the critical residues at the stereochemistry-gate loops of Brevibacillus agri dihydropyrimidinase for the production of l-homophenylalanine

Keywords: mutagenesis مواجه با سایت; l-Homophenylalanine; Dihydropyrimidinase; l-N-Carbamoylase; N-Acylamino acid racemase; Site-directed mutagenesis; Bioconversion

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Genetically engineering transferrin to improve its in vitro ability to deliver cytotoxins

Keywords: mutagenesis مواجه با سایت; Transferrin; Diphtheria toxin; Site-directed mutagenesis; Intracellular trafficking; Targeted cancer therapy

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Cysteine residues of Carnation Italian Ringspot virus p19 suppressor of RNA silencing maintain global structural integrity and stability for siRNA binding

Keywords: mutagenesis مواجه با سایت; p19; siRNA; RNA silencing; Cysteine alkylation; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

The ion channel activity of the SARS-coronavirus 3a protein is linked to its pro-apoptotic function

Keywords: mutagenesis مواجه با سایت; a.a.; amino acid; AO; acridine orange; ER; endoplasmic reticulum; ORF; opening reading frame; SARS-CoV; severe acute respiratory syndrome-coronavirus; Drosophila; Ion channel; Severe acute respiratory syndrome; Site-directed mutagenesis; Vero E6;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Binding specificity of locust odorant binding protein and its key binding site for initial recognition of alcohols

Keywords: mutagenesis مواجه با سایت; Odorant binding protein; Binding specificity; Locusta migratoria; Homology modeling; Molecular docking; Site-directed mutagenesis; Binding sites

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Restoring 3′–5′ exonuclease activity of thermophilic Geobacillus DNA polymerase I using site-directed mutagenesis in active site

Keywords: mutagenesis مواجه با سایت; 3′–5′ Exonuclease; Site-directed mutagenesis; Geobacillus sp. MKK; DNA polymerase

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

The anti-convulsant stiripentol acts directly on the GABAA receptor as a positive allosteric modulator

Keywords: mutagenesis مواجه با سایت; Anti-convulsant; GABA; Electrophysiology; Site-directed mutagenesis; Recombinant; Patch-clamp;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Role of the basic character of α-sarcin’s NH2-terminal β-hairpin in ribosome recognition and phospholipid interaction

Keywords: mutagenesis مواجه با سایت; Ribotoxin; Protein–lipid interaction; Ribosome recognition; Restrictocin; Electrostatic interaction; Ribonuclease; Site-directed mutagenesis; Fluorescence; Circular dichroism; Phospholipid vesicles aggregation

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Characterization of recombinant thiamine diphosphate-dependent phosphonopyruvate decarboxylase from Streptomyces viridochromogenes Tü494

Keywords: mutagenesis مواجه با سایت; Thiamine diphosphate; Phosphonopyruvate; Decarboxylase; Site-directed mutagenesis; Thiamine binding site

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Identifying catalytic residues in CPAF, a Chlamydia-secreted protease

Keywords: mutagenesis مواجه با سایت; Chlamydia; CPAF; Hidden Markov Models; HHPRED; MODELLER; Molecular modeling; Tricorn protease; Catalytic triad; Site-directed mutagenesis; Protein structure prediction

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه

Specificity versus catalytic potency: The role of threonine 44 in Escherichia coli dihydrodipicolinate synthase mediated catalysis

Keywords: mutagenesis مواجه با سایت; Dihydrodipicolinate synthase; Site-directed mutagenesis; (S)-Lysine biosynthesis; Catalysis; Protein dynamicsHTPA, (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid; DHDPS, dihydrodipicolinate synthase; DHDPR, dihydrodipicolinate reductase; (S)-ASA,

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2009 سفارش ترجمه

The transmembrane tyrosines Y56, Y91 and Y167 play important roles in determining the affinity and transport rate of the rabbit proton-coupled peptide transporter PepT1

Keywords: mutagenesis مواجه با سایت; Epithelia; Membrane transport; SLC15a1; Nutrient absorption; Protein structure-function; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2009 سفارش ترجمه

Exploring the acidotolerance of β-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus: an attractive enzyme for lactose bioconversion

Keywords: mutagenesis مواجه با سایت; β-Galactosidase; Acidotolerance; Site-directed mutagenesis; Molecular modeling; Lactose hydrolysis;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2009 سفارش ترجمه

The SV40 Capsid Is Stabilized by a Conserved Pentapeptide Hinge of the Major Capsid Protein VP1

Keywords: mutagenesis مواجه با سایت; WT, wild type; RQ-PCR, real-time quantitative PCRSV40; major capsid protein VP1; site-directed mutagenesis; computational binding prediction; protein structure–function

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2009 سفارش ترجمه

Functional Importance of Bacteriophage ϕ29 DNA Polymerase Residue Tyr148 in Primer-terminus Stabilisation at the 3′-5′ Exonuclease Active Site

Keywords: mutagenesis مواجه با سایت; ssDNA, single-stranded DNA; pNP-TMP, 5′-p-nitrophenyl ester of thymidine 5′-monophosphate; Pol Ik, Klenow fragment of DNA polymerase Iϕ29 DNA polymerase; 3′-5′ exonuclease; site-directed mutagenesis; ssDNA binding; strand displacement

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2009 سفارش ترجمه

The mechanism causing the difference in kinetic properties between rat CYP2D4 and human CYP2D6 in the oxidation of dextromethorphan and bufuralol

Keywords: mutagenesis مواجه با سایت; CYP2D4; CYP2D6; Bufuralol; Dextromethorphan; Nonlinear kinetics; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2009 سفارش ترجمه

Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants

Keywords: mutagenesis مواجه با سایت; Monoterpene synthase; Sesquiterpene synthase; Terpene synthase reaction mechanism; Site-directed mutagenesis; Terpene synthase structure; Structure-function correlation;

دانلود رایگان متن کامل مقاله ISI 17 صفحه سال انتشار : 2009 سفارش ترجمه

Molecular cloning, expression, characterization and mutation of Plasmodium falciparum guanylate kinase

Keywords: mutagenesis مواجه با سایت; GK, guanylate kinase; PfGK, Plasmodium falciparum guanylate kinase; rPfGK, recombinant Plasmodium falciparum guanylate kinasePlasmodium falciparum; Guanylate kinase; Site-directed mutagenesis; Drug targets

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2008 سفارش ترجمه

Site-directed, Ligase-Independent Mutagenesis (SLIM) for highly efficient mutagenesis of plasmids greater than 8kb

Keywords: mutagenesis مواجه با سایت; Site-directed mutagenesis; Inverse PCR; Ligase-free; 5′-adapter

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2008 سفارش ترجمه

Thr but Asn of the N-glycosylation sites of PrP is indispensable for its misfolding

Keywords: mutagenesis مواجه با سایت; Prion protein; N-linked glycosylation; Conversion; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2008 سفارش ترجمه

Identification and analysis of novel functional sites in human GD3-synthase

Keywords: mutagenesis مواجه با سایت; CMP-NeuAc; cytidine 5′-monophospho-N-acetylneuraminic acid; ST3Gal; ST3 β-galactoside α2,3-sialyltransferase; ST6Gal; ST6 β-galactoside α2,6-sialyltransferase; ST6GalNAc; ST6 (α-N-acetylneuraminyl-2,3-β-galactosyl-1,3)-N-acetylgalactosaminide α2,

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2008 سفارش ترجمه

Homotropic allosteric control in clostridial glutamate dehydrogenase: Different mechanisms for glutamate and NAD+?

Keywords: mutagenesis مواجه با سایت; GDH, glutamate dehydrogenase (NAD+-dependent) EC 1.4.1.2; h, Hill coefficientGlutamate dehydrogenase; Negative cooperativity; Allosteric mechanism; Trp/Phe mutants; Homotropic interaction; Site-directed mutagenesis; Hill coefficients

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2008 سفارش ترجمه

Identification of two novel β-mannosidosis-associated sequence variants: Biochemical analysis of β-mannosidase (MANBA) missense mutations

Keywords: mutagenesis مواجه با سایت; β-Mannosidase (MANBA); β-Mannosidosis; Mutation analysis; Site-directed mutagenesis; Biochemical characterization;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2008 سفارش ترجمه

Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae

Keywords: mutagenesis مواجه با سایت; High-throughput; Protein expression; Site-directed mutagenesis; Eukaryotic membrane protein; Crystallization; Saccharomyces cerevisiae; GFP-fusion; G protein-coupled receptor; Taste

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2008 سفارش ترجمه

Substitution of DNA-contacting amino acids with functional variants in the Gata-1 zinc finger: A structurally and phylogenetically guided mutagenesis

Keywords: mutagenesis مواجه با سایت; Gata; Zinc finger; DNA-binding domain; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2008 سفارش ترجمه

A new T677C mutation of the aspartoacylase gene encodes for a protein with no enzymatic activity

Keywords: mutagenesis مواجه با سایت; Canavan disease; N-acetylaspartate; N-acetylaspartoacylase; T677C mutation; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2008 سفارش ترجمه

Substitution of strictly conserved Y111 in catalase–peroxidases: Impact of remote interdomain contacts on active site structure and catalytic performance

Keywords: mutagenesis مواجه با سایت; Catalase–peroxidase; KatG; Heme; Interdomain interactions; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2008 سفارش ترجمه

Molecular modeling of the human multidrug resistance protein 1 (MRP1/ABCC1)

Keywords: mutagenesis مواجه با سایت; Multidrug resistance protein; ABC transporter; Organic anion transporter; Homology modeling; MRP1; Sav1866; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2008 سفارش ترجمه