Insights into the catalytic properties of bamboo vacuolar invertase through mutational analysis of active site residues

Invertase catalyzes the hydrolysis of sucrose to glucose and fructose. A theoretical structure of bamboo vacuolar invertase Boβfruct3 was constructed by homology modeling. The roles of amino acid residues that are conserved in or around the active site of the GH32 enzymes were studied by site-directed mutagenesis.