Engineering the residues on “A” surface and C-terminal region to improve thermostability of nitrilase
Keywords: ترموستاتا; 1-CA; 1-cyanocyclohexaneacetic acid; 1-CN; 1-cyanocyclohexylacetonitrile; E. coli; Escherichia coli; HPLC; high performance liquid chromatography; LB broth; Luria-Bertani broth; SDS-PAGE; dodecyl sulfate sodium salt-polyacrylamide gel electrophoresis; Nit