Keywords: tris (2-carboxyethyl) phosphine hydrochloride; CuAAC; Copper-Catalyzed Azide-Alkyne Cycloadditions; PhSH; thiophenol; alloc; allylcarbamate group; DAPI; 4â²,6-diamidine-2â²-phenylindole; EtBr; ethidium bromide; TON; turnover number; GSH; glutathione; PBS; phosphate buffered saline; TPP; triphenylp
مقالات ISI tris (2-carboxyethyl) phosphine hydrochloride (ترجمه نشده)
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Keywords: tris (2-carboxyethyl) phosphine hydrochloride; RSSH; generic hydropersulfide; Cys-SSH; cysteine hydropersulfide; GSSH; glutathione hydropersulfide; DTT; dithiothreitol; TCEP; tris(2-carboxyethyl)phosphine hydrochloride; NEM; N-ethyl maleimide; DTNB; 5,5â²-dithiobis-(2-nitrobenzoic acid); FDP; fluores
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; AF; affinity chromatography; BSA; bovine serum albumin; CRMs; certified reference materials; Cys; cysteine; DNA; deoxyribonucleic acid; eGPx; extracellular glutathione peroxidase; GPx; glutathione peroxidase; GSH; reduced glutathione; HMM; high molecular
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; AD; Alzheimer's disease; AEC; anionic exchange chromatography; DNA; deoxyribonucleic acid; DTT; dithiothreitol; ESI; electrospray ionization; EC; extra cellular; HPLC; high-performance liquid chromatography; ICP-MS; inductively coupling plasma-mass spec
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Disulfide bonds; Liquid chromatography and mass spectrometry; Tris(2-carboxyethyl)phosphine hydrochloride; Antibody; Lysozyme;
A modified clear-native polyacrylamide gel electrophoresis technique to investigate the oligomeric state of MBP-5-HT3A-intracellular domain chimeras
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Clear-native PAGE; Multi-angle light scattering; Native gel electrophoresis; Protein oligomerization; Protein quaternary structures; Size exclusion chromatography; 5-HT3A; serotonin type 3A; 6-AHA; 6-aminohexanoic acid; BN-PAGE; blue-native polyacrylamide
Ordered opening of LDL receptor binding domain of human apolipoprotein E3 revealed by hydrogen/deuterium exchange mass spectrometry and fluorescence spectroscopy
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Apolipoprotein E3; Helix bundle opening; LDL receptor; Hydrogen deuterium exchange mass spectrometry; Fluorescence spectroscopy; Lipoprotein binding; apoE3; apolipoprotein E3; CD; circular dichroism; CT; C-terminal; GdnHCl; guanidine hydrochloride; FI; fl
Target discovery of cytotoxic withanolides from Physalis angulata var. villosa via reactivity-based screening
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; RBS; reactivity-based screening; AEO; 2(3)-en-1-one in ring A; BE; 5β,6β-epoxy in ring B; LC-Q-TOF-MS; liquid chromatography quadrupole-time-of-flight mass spectrometry; LC-MS; liquid chromatography-mass spectrometry; MeOH; methanol; CH3CN; acetonit
Evidence for functional and regulatory cross-talk between the tricarboxylic acid cycle 2-oxoglutarate dehydrogenase complex and 2-oxoadipate dehydrogenase on the l-lysine, l-hydroxylysine and l-tryptophan degradation pathways from studies in vitro
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; hOGDHc; human 2-oxoglutarate dehydrogenase complex; hE1o; 2-oxoglutarate dehydrogenase, the first E1 component of hOGDHc; hE2o; dihydrolipoyl succinyltransferase, the second E2 component of hOGDHc; hE3; dihydrolipoyl dehydrogenase, the third E3 component
Effect of steaming and sous vide processing on the total phenolic content, vitamin C and antioxidant potential of the genus Brassica
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; ANOVA; Analysis of variance; DPPH; 2,2-Diphenyl-1-picrylhydrazyl; FRAP; Ferric reducing antioxidant power; h0; Hue angle; HPLC; High-performance liquid chromatography; S.D; Standard deviation; TPTZ; 2,4,6-Tris(2-pyridyl)-s-triazine; UV; Ultraviolet; TCEP;
Selective disulfide reduction for labeling and enhancement of Fab antibody fragments
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Monoclonal antibody; Selective disulfide reduction; Fab fragments; TCEP agarose; Sulfhydryl modification; Antibody conjugation; mAb; monoclonal antibody; h2E2; humanized monoclonal antibody against cocaine; Endo Lys-C; Endoproteinase Lys-C; IAM; iodoaceta
Structural characterization of expressed monoclonal antibodies by single sample mass spectral analysis after IdeS proteolysis
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Monoclonal antibody; Post-translational modifications; IdeS cleavage; Tributylphosphine reduction; Mass spectrometry; Glycoform determination; mAb; monoclonal antibody; h2E2; humanized monoclonal antibody against cocaine; IdeS protease; Immunoglobulin G-d
A class-A GPCR solubilized under high hydrostatic pressure retains its ligand binding ability
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; G protein-coupled receptor; High hydrostatic pressure; Pheromone biosynthesis-activating neuropeptide receptor; Solubilization; Ligand binding ability; AEBSF; 4-(2-aminoethyl)benzensulfonyl fluoride; AP; ambient pressure; CBB; Coomassie Brilliant Blue; CH
Spectroscopic investigation of auranofin binding to zinc finger HIV-2 nucleocapsid peptides
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Auranofin; 1-Thio-β-d-glucopyranosatotriethylphosphine gold-2,3,4,6-tetraacetate; TCEP; Tris(2-carboxyethyl)phosphine hydrochloride; PAR; 4-(pyridyl-2-azo)-resorcinol; Auranofin; Zinc finger; HIV-2; Mass spectrometry; CD; Fluorescence;
Choline sulfatase from Ensifer (Sinorhizobium) meliloti: Characterization of the unmodified enzyme
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; COS; E. meliloti choline-O-sulfatase; FGly; α-formylglycine; FGE; α-formylglycine-generating enzyme; anSME; anaerobic sulfatase maturing enzyme; pNPS; p-nitrophenyl sulfate; MUS; 4-methylumbelliferyl sulfate; ITC; isothermal titration calorimetry; DLS;
PEGylation of the peptide Bac7(1-35) reduces renal clearance while retaining antibacterial activity and bacterial cell penetration capacity
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Antimicrobial peptide; Proline-rich; PEGylation; Antibacterial activity; Cell penetrating; Optical imaging; Boc2O; Di-t-butyl dicarbonate; Et2O; ethyl ether; CFU; colony forming unit; Cmc; S-carboxymethyl-cysteine; DIEA; N,N-Diisopropylethylamine; HCTU; 2
Results of an international transferability study of the BINACLE (binding and cleavage) assay for in vitro detection of tetanus toxicity
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Tetanus neurotoxin; Toxoid vaccines; In vitro toxicity assay; Transferability study; BINACLE; binding and cleavage; BSA; bovine serum albumin; ELISA; enzyme-linked immunosorbent assay; IVI; Institute of Virology and Immunology; NIBSC; National Institute
Solution conditions define morphological homogeneity of α-synuclein fibrils
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; αSyn; α-synuclein; AFM; Atomic Force Microscopy; A140C αSyn; substitution mutant of αSyn with residue 140 mutated from alanine to cysteine; EDTA; ethylenediaminetetraacetic acid; TCEP; tris(2-carboxyethyl)phosphine hydrochloride; DTT; dithiothreitol;
Mapping the UDP-N-acetylglucosamine regulatory site of human glucosamine-6P synthase by saturation-transfer difference NMR and site-directed mutagenesis
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Glucosamine-6-phosphate synthase; UDPGlcNAc binding site; Glutamine amidotransferase; STD-NMR; Allosteric regulation; Transferred NOE; Fru6P; d-fructose-6-phosphate; Glc6P; d-glucose-6-phosphate; GlcN6P; d-glucosamine-6-phosphate; hGfat; human l-glutamine
Membrane targeting of palmitoylated Wnt and Hedgehog revealed by chemical probes
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; Hh; Hedgehog; Shh; Sonic Hedgehog; Alk-C16; Ï-alkynyl palmitic acid; BPA; bromopalmitic acid; PA; palmitic acid; IWP-2; inhibitor of Wnt production; HEK; human embryonic kidney; DMEM; Dulbecco's modified Eagle's medium; FBS; fetal bovine serum; BSA;
Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; B35; Brij-35; B58; Brij-58; B78; Brij-78; B98; Brij-98; CD; circular dichroism; CF; cell-free; CHAPS; 3-[(3-cholamidopropyl) dimethylammonio]-1-propansulphonat; CHS; cholesteryl hemisuccinate tris salt; CMC; critical micellar concentration; CV; column vol
Detection of neuronal growth inhibitory factor (metallothionein-3) in polyacrylamide gels and by Western blot analysis
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; AD; Alzheimer's disease; AP; alkaline phosphatase; BCIP; 5-bromo-4-chloro-3-indolyl phosphate p-toluidine salt; DTT; 1,4-dithio-dl-threitol; Hepes; 4-(2-hydroxyethyl)-piperazine-1-ethanesulfonic acid; IPTG; isopropyl β-d-1-thiogalactopyranoside; mBrB; mo
Targeting liposomes with protein drugs to the blood-brain barrier in vitro
Keywords: tris (2-carboxyethyl) phosphine hydrochloride; BBB; blood-brain barrier; BCEC; brain capillary endothelial cells; EPC-35; egg-phosphatidyl choline, partially saturated; HBS; HEPES buffered saline; HRP; horseradish peroxidase; PEG; polyethylene glycol (MW 2000); PBS; phosphate buffered saline; p.i.;