Keywords: APP; amyloid precursor protein; CASP6; caspase-6; Co-IP; co-immunoprecipitation; CRM; cis-regulatory module; DDBJ; DNA Data Bank of Japan; EMBL; European Molecular Biology Laboratory; HD; Huntington disease; HTT; huntingtin; IPI; International Protein Ind
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Keywords: ACT; actin; AD; activation domain; BiFC; bimolecular fluorescence complementation; CaM; calmodulin; cDNA; complementary DNA; Co-IP; co-immunoprecipitation; DBD; DNA-binding domain; EMSA; electrophoretic mobility shift assay; GFP; green fluorescent protein
Keywords: Prostacyclin receptor; Estrogen receptor; Sterol response element binding protein (SREBP); PDZK1(PDZ-domain-containing protein 1); IKEPP (intestinal and kidney enriched PDZ protein); C/EBP, CCAAT/enhancer binding protein; Rab11; Gene; Cardiovascular; AoSM
Keywords: 2DE; two-dimensional gel electrophoresis; APAF1; apoptotic protease-activating factor 1; CDWE; cell wall-degrading enzymes; CED4; cell death protein 4; ChIP seq; chromatin immunoprecipitation-sequencing; CoIP; co-immunoprecipitation; DIGE; fluorescence di
Overexpression of a repressor MdMYB15L negatively regulates anthocyanin and cold tolerance in red-fleshed callus
Keywords: Repressor; MdMYB15L; Anthocyanin; Cold tolerance; Red-fleshed callus; ANS; anthocyanin synthase; UFGT; UDP-glucose:flavonoid 3- glucosyltransferase; GFP; green fluorescent protein; GST; glutathione S-transferase; His; histidine; Anti; antibody; MS; Murash
Networks of protein-protein interactions among structural proteins of budded virus of Bombyx mori nucleopolyhedrovirus
Keywords: AcMNPV; Autographa californica multiple nucleopolyhedrovirus; B.mori; Bombyx mori; BmNPV; Bombyx mori nucleopolyhedrovirus; BV; budded virus; C42; BV/ODV-C42; Co-IP; co-immunoprecipitation; E.coli; Escherichia coli; E25; ODV-E25; E18; ODV-E18; EC27; ODV-E
Neuregulin 1 discovered as a cleavage target for the HCV NS3/4A protease by a microfluidic membrane protein array
Keywords: HCV; hepatitis C virus; HCC; hepatocellular carcinoma; NS; nonstructural protein; TIR; toll/interleukin 1 receptor; TRIF; (TIR)-domain-containing adapter-inducing interferon-β; IIR; innate immune response; RIG-I; retinoic acid inducible gene; MAVS; mitoc
Insights into pathogenesis of five novel GCK mutations identified in Chinese MODY patients
Keywords: GCK; Glucokinase; GKRP; Glucokinase regulatory protein; GSIS; Glucose-stimulated insulin secretion; G6P; Glucose-6-phosphate; GKAs; Glucokinase activators; MODY; Maturity-onset diabetes of the young; mGCK; Mutant GCK; OHA; Oral hypoglycaemic agents; WT GC
Soybean TCP transcription factors: Evolution, classification, protein interaction and stress and hormone responsiveness
Keywords: Soybean TCP transcription factor; Plant growth and development; Protein interaction; Stress response; Hormone signals; Transcript expression; ABA; abscisic acid; BR; brassinolide; MeJA; methyl jasmonate; qRT-PCR; quantitative real-time PCR; SA; salicylica
The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha
Keywords: aa; amino acid; Ab; antibody(ies); BSA; bovine serum albumin; cDNA; DNA complementary to RNA; co-IP; IP by a second Ab of a complex precipitated by a primary Ab; CTU; complex transcription unit; GCOM1; GRINL1A combined gene 1 aka and originally assigned a
Interaction of Mal de RÃo Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay
Keywords: MRCV; viroplasm; PEST sequence; Reoviridae; Y2H;
Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis
Keywords: AD; activation domain; CGNP; cerebellar granular neuron progenitor cells; CIP; calf intestinal alkaline phosphatase; DB; DNA-binding domain; EGCG; epigallocatechin gallate; FFPE; formalin-fixed, paraffin-embedded; H&E; hematoxylin/eosin staining; Hh; Hedg
Physical and functional interaction of Snapin with Cav1.3 calcium channel impacts channel protein trafficking in atrial myocytes
Keywords: AF; atrial fibrillation; LTCC; L-type calcium channels; CICR; calcium induced calcium release; Y2H; yeast two-hybrid; SNARE; soluble N-ethylmaleimide-sensitive fusion attachment protein; Cav1.3; Snapin; Membrane trafficking; SNARE; Atrial fibrillation;
CommunicationPRMT7 Interacts with ASS1 and Citrullinemia Mutations Disrupt the Interaction
Keywords: PRMT; protein arginine methyltransferase; Y2H; yeast two-hybrid; ASS1; argininosuccinate synthetase; co-IP; co-immunoprecipitation; GST; glutathione S-transferase; GFP; green fluorescent protein; CD; circular dichroism; BSA; bovine serum albumin; protein
Proteomics analysis of the endogenous, constitutive, leaf SUMOylome
Keywords: 3D gel electrophoresis; Post translational modification; SUMOylation; Endogenous SUMO conjugates; Solanum tuberosum; IMAC; immobilized metal ion affinity chromatography; IAA; iodoacetamide; NEM; N-ethylmaleimide; PTM; post-translational modification; TCA;
Molecular cloning and functional characterization of CoFT1, a homolog of FLOWERING LOCUS T (FT) from Camellia oleifera
Keywords: CaMV; cauliflower mosaic virus; DAG; days after germination; LDs; long-day conditions; SDs; short-day conditions; ZT; Zeitgeber time; ORF; open reading frame; gDNA; genomic DNA; qPCR; quantitative real-time PCR; TSS; transcription start site; GFP; green f
Soybean Golgi SNARE 12 protein interacts with Soybean mosaic virus encoded P3N-PIPO protein
Keywords: Interaction; Soybean mosaic virus; P3N-PIPO; Golgi SNARE 12; Yeast two hybrid; Bimolecular fluorescence complementation; GOS12; Golgi SNARE 12; BiFC; Bimolecular fluorescence complementation; MP; movement protein; PD; plasmodesmata; SMV; Soybean mosaic vi
UBE2D2 is not involved in MuRF1-dependent muscle wasting during hindlimb suspension
Keywords: UPS; ubiquitin proteasome system; HS; hindlimb suspension; MuRF1; muscle ring finger protein-1; UBE2D2; ubiquitin-conjugating enzyme E2 D2; UBE2B; ubiquitin-conjugating enzyme E2 B; MHC; myosin heavy chain; SPR; surface plasmon resonance; Y2H; yeast two-h
EIN3 interferes with the sulfur deficiency signaling in Arabidopsis thaliana through direct interaction with the SLIM1 transcription factor
Keywords: aa; amino acids; BD; basic domain; dS; sulfur deficient; EBS; EIN3 binding site; EMSA; electrophoretic mobility shift assay; JA; jasmonic acid; qRT-PCR; quantitative real-time PCR; sS; sulfur sufficient; TEBS; TEIL binding site; wt; wild type; Y2H; yeast
OsSET7, a homologue of ARABIDOPSIS TRITHORAX-RELATED protein that plays a role in grain elongation regulation in rice
Keywords: ATXR; ARABIDOPSIS TRITHORAX-RELATED; BiFC; Bimolecular Fluorescence Complementation; CDS; coding sequence; HKMT; Histone Lysine Methyltransferase; SET; Suppressor of variegation, Enhancer of zeste and Trithorax; PcG; Polycomb Group; PCNA; Proliferating Ce
RNF138 interacts with RAD51D and is required for DNA interstrand crosslink repair and maintaining chromosome integrity
Keywords: HR; homologous recombination; CFA; colony forming assay; NT; N-terminus; ssDNA; single-stranded DNA; ICL; interstrand crosslink; DSB; double-strand break; MEF; mouse embryo fibroblasts; MMC; mitomycin C; MMS; methyl methanesulfonate; PTM; post-translation
Molecular and genetic characterization of a self-compatible apple cultivar, 'CAU-1'
Keywords: aa; amino acid; BiFC; bimolecular fluorescence complementation; DAPI; 4â², 6-diamidino-2-phenylindole; ER; endoplasmic reticulum; FITC; fluorescein isothiocyanate; GM; germination medium; PPM; pollen-part mutation; RT-PCR; real-time PCR; SC; self-compati
SGIP1 alters internalization and modulates signaling of activated cannabinoid receptor 1 in a biased manner
Keywords: Seven transmembrane receptors; G-protein coupled receptors; Cannabinoid receptor 1; Protein-protein interactions; Bias signaling; Receptor endocytosis; SGIP1; src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1; CC
Libraries for two-hybrid screening of yeast and hyphal growth forms in Zymoseptoria tritici
Keywords: Protein-protein interaction; Pathogenic fungi; Wheat disease; Septoria tritici blotch; Mycosphaerella graminicola; Y2H; yeast two hybrid; DNA; deoxyribonucleic acid; cDNA; complementary deoxyribonucleic acid; gDNA; genomic deoxyribonucleic acid; RNA; ri
Cyclin C interacts with steroid receptor coactivator 2 and upregulates cell cycle genes in MCF-7 cells
Keywords: 6His; hexahistidine tag; aa; amino acids; Bcl-2; apoptosis regulator B-cell CLL/lymphoma 2; cAMP; cyclic adenosine monophosphate; CCNC; cyclin C; CCPG1; cell cycle progression protein 1; CDK6; cyclin-dependent kinase 6; cDNA; coding DNA; CXCR4; chemokine
Conserved vacuolar H+-ATPase subunit B1 improves salt stress tolerance in apple calli and tomato plants
Keywords: MdVHA-B1; apple vacuolar H+-ATPase subunit B1; Y2H; yeast two-hybrid; BiFC; bimolecular fluorescence complementation; SOS signal pathway; salt overly sensitive; MdVHA-B1; Salt tolerance; Apple; V-ATPase; SOS signal pathway;
Protein sHSP26 improves chloroplast performance under heat stress by interacting with specific chloroplast proteins in maize (Zea mays)
Keywords: Co-IP; co-immunoprecipitation; OEC; oxygen evolving complex; PS II; photosystem II; PEG; polyethylene glycol; PQ; paraquat; RNAi; RNA interference; Y2H; yeast two-hybrid; sHSP26; Chloroplast proteins; Heat stress; Heat shock protein; Maize protoplast; Pro
Disruption of SUMO-targeted ubiquitin ligases Slx5-Slx8/RNF4 alters RecQ-like helicase Sgs1/BLM localization in yeast and human cells
Keywords: HU; hydroxyurea; Y2H; yeast-2-hybrid; DSB; double-strand break; HR; homologous recombination; STUbL; SUMO-targeted ubiquitin ligase; SUMO; small ubiquitin-like modifier; YFP; yellow fluorescent protein; CFP; cyan fluorescent protein; RFP; red fluorescent
Arabidopsis dynamin-related proteins, DRP2A and DRP2B, function coordinately in post-Golgi trafficking
Keywords: BiFC; bi-molecular fluorescent complementation; CLSM; confocal laser scanning microscopy; CoIP-MS; co-immunoprecipitation mass spectrometry; DRP; dynamin-related protein; GED; GTPase-effector domain; Lat B; latrunculin B; ORY; oryzalin; PH; pleckstrin hom
Arabidopsis thaliana plastoglobule-associated fibrillin 1a interacts with fibrillin 1b in vivo
Keywords: FBN; fibrillin; Y2H; yeast Two-Hybrid; BiFC; bimolecular fluorescence complementation; CTP; chloroplast transient peptide; Fibrillin; Plastoglobule; Protein-protein interaction; Arabidopsis;
p32, a novel binding partner of Mcl-1, positively regulates mitochondrial Ca2+ uptake and apoptosis
Keywords: Co-IP; co-immunoprecipitation; DMSO; dimethyl sulfoxide; GFP; green fluorescent protein; RuRed; Ruthenium Red; YFP; yellow fluorescent protein; Y2H; Yeast Two Hybrid; Apoptosis; Mcl-1; p32; Mitochondria; Calcium; Mitochondrial calcium uniporter;
Progranulin directly binds to the CRD2 and CRD3 of TNFR extracellular domains
Keywords: PGRN; progranulin; TNFR; tumor-necrotizing factor receptor; CRD; cysteine-rich domains; BMDM; bone marrow-derived macrophages; SPR; Surface plasmon resonance; FTLD; frontotemporal lobar degeneration; Y2H; yeast two-hybrid; HRP; horseradish peroxidase; PGR
Improvement of stress tolerance in plants by genetic manipulation of mitogen-activated protein kinases
Keywords: ABA; abscisic acid; ACC; 1-amino-cyclopropane-1-carboxylic acid; ACS6; ACC synthase 6; BAK1; BRI1-associated kinase 1; CTR1; constitutive triple response 1; EF-Tu; elongation factor thermo-unstable; ETI; effector-triggered immunity; ETR1; ethylene respons
An integrated proteomics and bioinformatics analyses of hepatitis B virus X interacting proteins and identification of a novel interactor apoA-I
Keywords: HBV; hepatitis B virus; HBx; hepatitis B virus X proteins; apoA-I; apolipoprotein A-I; GO; Gene Ontology; GST; glutathione S-transferase; DMPC; dimyristoyl-phosphatidylcholine; DNP; 2,4-dinitrophenylhydrazine; ELISA; enzyme-linked immunosorbent assay; HBe
Nuclear α1-Antichymotrypsin Promotes Chromatin Condensation and Inhibits Proliferation of Human Hepatocellular Carcinoma Cells
Keywords: Liver Cancer; Nuclear Factor; Mouse Model; DNA Binding; AAV; adeno-associated virus; α1-ACT; antichymotrypsin; APP; acute-phase protein; APR; acute-phase response; DOX; doxycycline; EGF; epidermal growth factor; HCC; hepatocellular carcinoma; MENT; myelo
Beyond hairballs: The use of quantitative mass spectrometry data to understand protein-protein interactions
Keywords: AP-MS; affinity purification coupled to mass spectrometry; Y2H; yeast two hybrid; TAP; Tandem Affinity Purification; QconCAT; quantification concatemer; STRIPAK; STRiatin Interacting Phosphatase And Kinase; LUMIER; luminescence-based mammalian interactome
The application of modular protein domains in proteomics
Keywords: pTyr; phosphotyrosine; PTM; post-translational modification; MS; mass spectrometry; PSSM; position specific scoring matrix; SH2; Src homology 2; SH3; Src homology 3; WW; tryptophan tryptophan; PDZ; post synaptic density protein 95-disks large protein 4-zo
A regulatory cascade of three transcription factors in a single specific neuron, DVC, in Caenorhabditis elegans
Keywords: AD; activation domain; ANOVA; analysis of variance; Bp; base pairs; ChR2; channelrhodopsin-2; DAPI; 4â²,6-diamidino-2-phenylindole; DB; DNA binding domain; DIC; differential interference contrast; DNA; deoxyribonucleic acid; EST; expressed sequence tag;
Interaction of the human prostacyclin receptor and the NHERF4 family member intestinal and kidney enriched PDZ protein (IKEPP)
Keywords: DDO; double drop-out; FTI; farnesyl transferase inhibitor; GPCR; G protein-coupled receptor; HA; hemagglutinin; hIP; human prostacyclin receptor; HUVEC; human umbilical vein endothelial cell; IKEPP; intestinal and kidney enriched PDZ protein; IP; prostacy
The plant cell death suppressor Adi3 interacts with the autophagic protein Atg8h
Keywords: HR; hypersensitive response; MAPK; mitogen activated protein kinase; ORF; open reading frame; PCD; programmed cell death; Pst; Pseudomonas syringae pv. tomato; VIGS; virus induced gene silencing; Y2H; yeast two-hybrid assay; Adi3; Atg8h; Autophagy; Progra
Investigating a macromolecular complex: The toolkit of methods
Keywords: AUC; analytical ultracentrifugation; ChIP; chromatin immunoprecipitation; co-IP; co-immunoprecipitation; cryoEM; cryoelectron microscopy; cryoET; cryoEM tomography; DSC; differential scanning calorimetry; EPR; electron paramagnetic resonance; FAIM; fluore
14-3-3 proteins in plant physiology
Keywords: ABA; abscisic acid; GA; giberellic acid; BR; brassinosteroid; Y2H; yeast-two hybrid; PCD; programmed cell death; DRM; detergent resistant membrane; BiFC; bimolecular fluorescence complementation; AP; affinity purification; 14-3-3 signaling; Plant stress;
TLE1 Modifies the Effects of NOD2 in the Pathogenesis of Crohn's Disease
Keywords: Intestine; Inflammation; Genetic Analysis; IBD Risk; CI; confidence interval; FIS1; fission 1 (mitochondrial outer membrane) homolog; GALNT2; UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2; GRIM1; Gene associated with r
Interaction of angio-associated migratory cell protein with the TPα and TPβ isoforms of the human thromboxane A2 receptor
Keywords: AAMP; angio-associated migratory cell protein; AoSMC; aortic smooth muscle cell; CoASMC; coronary artery smooth muscle cell; C-tail; carboxyl-terminal tail; FBS; foetal bovine serum; GPCR; G protein-coupled receptor; GST; glutathione-S-transferase; HA; ha
AmGAI-like interacts with ROSINA, a putative transcriptional regulator of DEFICIENS in Antirrhinum majus
Keywords: GRAS family proteins; MADS-box; DEFICIENS; B-function; Y2H; Flower development and Antirrhinum majus;
Quantitative Y2H screening: Cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter
Keywords: Quantitative screening; Y2H; Fungal beta-galactosidase; LacA; Directed evolution
Interaction between cartilage oligomeric matrix protein and extracellular matrix protein 1 mediates endochondral bone growth
Keywords: COMP; cartilage oligomeric matrix protein; ECM1; extracellular matrix protein 1; EGF; epidermal growth factor; ADAMTS; a disintegrin and metalloproteinase with thromospondin motifs; GEP; granulin-epithelin precursor; GST; glutathione S-transferase; PCR;
The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
Keywords: Y2H; yeast two-hybrid; ONPG; ortho-nitrophenyl-β-galactoside; SOEing PCR; splice overlap extension PCR; CPSF; cleavage and polyadenylation specificity factor; FIP; Factor Interacting with PAP; Polyadenylation factors; mRNA processing; Protein interaction
An interaction between leukocyte cell-derived chemotaxin 2 and transferrin of ayu, Plecoglossus altivelis
Keywords: Ayu; LECT2; Protein interaction; Transferrin; Y2H
A surface display yeast two-hybrid screening system for high-throughput protein interactome mapping
Keywords: Surface display; Y2H; yEGFP; High throughput; cDNA library screening