Comparison of the efficiency of chitinous and ligneous substrates in metal and sulfate removal from mining-influenced water

Mining-influenced water (MIW) remediation is challenging, not only due to its acidity and high metal content, but also due to its presence in remotely located mine sites with difficult surrounding environments. An alternative to common remediation technologies, is the use of sulfate-reducing bacteria (SRB) to achieve simultaneous sulfate reduction and metal removal in on-site anaerobic passive systems. In these systems, the organic carbon source (substrate) selection is critical to obtaining the desired effluent water quality and a reasonable treated volume. In this study, we evaluated the use of two different substrates: a chitinous product obtained from crushed crab shells, and a more traditional ligneous substrate. We put the substrates, both with and without water pretreatment consisting of aeration and pH adjustment, in anaerobic experimental columns. The treatment with the chitinous substrate was more effective in removing metals (Al, Cu, Fe, Cd, Mn, Zn) and sulfate for a longer period (458 days) than the ligneous substrate (78 days) before suffering Zn breakthrough. The reactors fed with pretreated water had longer operational periods and lower metals and sulfate concentrations in the effluent than those with untreated influent water. Zn was consistently removed to levels <0.3 mg/L for 513 days in the chitinous substrate columns, while levels <0.3 mg/L were maintained for only 140 days in the ligneous substrate pretreated column. The highest sulfate removal rates achieved in this study were in the range of 5-6 mol/m3/d for the chitinous substrate and 1-2 mol/m3/d for the ligneous substrate. Overall, the chitinous substrate proved to be more efficient in the removal of all the aforementioned metals and for sulfate when compared to the ligneous substrate. This could be the determinant when selecting a substrate for passive systems treating acidic MIW, particularly when Zn and Mn removal is necessary.