کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10142974 1646126 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tissue-specific and intracellular localization of indican synthase from Polygonum tinctorium
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Tissue-specific and intracellular localization of indican synthase from Polygonum tinctorium
چکیده انگلیسی
To examine intracellular localization of PtIGS, crude leaf extracts were separated into cytosol and microsome fractions, and found PtIGS in the cytosol and in microsome fractions. Furthermore, microsomal PtIGS was soluble in the presence of detergents and urea and was strongly associated with membranes. Finally, we confirmed endoplasmic reticulum (ER) membrane localization of PtIGS using ultracentrifugation with a sucrose density gradient. These data suggest that PtIGS interacts with some kind of proteins on ER membranes to certainly carry out a delivery of substrate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 132, November 2018, Pages 138-144
نویسندگان
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