کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10153070 | 1666193 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impedimetric biosensor for detection of cancer cells employing carbohydrate targeting ability of Concanavalin A
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The successful development of selective detection of cancer cells from normal cells is a highly demanded but challenging task. Herein, we have developed a rapid and label-free impedimetric biosensor for quantitative determination of cancer cells with high glycoprotein expression. Homogenously distributed 2-9â¯nm graphene quantum dots (GQDs) was anchored on the surface Fe3O4 through covalent bonding. Concovalin A (ConA) was then adhered onto GQDs by physical mixing to fabricate ConA-GQD@Fe3O4 nanosensing probe. A good dynamic range in the cell concentration of 5â¯Ãâ¯102-1â¯Ãâ¯105 cells mL-1 with LOD values of 246 and 367 cells mL-1 for HeLa and MCF-7, respectively, is obtained. The impedimetric responses to the cancerous HeLa and MCF-7 cells are 16.7 and 13.1 times higher than those of their original sensor electrodes. However, the interaction between sensing probe and normal MCF-10 and bEnd.3 cells is negligible, confirming the specific selectivity toward cancer cells. Cellular uptake images as well as in-vitro cytotoxicity corroborates the electrochemical results. In addition, the successful detection of cancer cells in human serum and circulating tumor cells in blood sample envisions the feasibility of using ConA-GQD@Fe3O4 as the nanosensing probe for clinically early diagnosis of cancer cells with high glycoprotein expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 122, 30 December 2018, Pages 95-103
Journal: Biosensors and Bioelectronics - Volume 122, 30 December 2018, Pages 95-103
نویسندگان
Ankan Dutta Chowdhury, Akhilesh Babu Ganganboina, Enoch Y. Park, Ruey-an Doong,