کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10154432 | 1666297 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reduction of the extra-column band dispersion by a slow transport of a sample band from the injector to the column in isocratic reversed-phase liquid chromatography
ترجمه فارسی عنوان
کاهش پراکندگی باند اضافی ستون با انتقال آهسته یک باند نمونه از انژکتور به ستون در کروماتوگرافی مایع فاز عقب
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کلمات کلیدی
اثر اضافی ستون، پراکندگی باند، تعداد صفحات، فضای قبل از ستون، برنامه نرخ جریان، حمل و نقل آهسته یک باند نمونه،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Extra-column band dispersion during the transport of a sample band from the injector to the column can be reduced by a flow rate program starting with a low flow rate until the sample band has approached to, or just entered into the column, followed by an increased flow rate suitable for the solute separation in the column. Such a sample introduction method increased the plate counts of a 50âmm long column, 1.0 or 2.1âmmID, especially for early-eluting solutes by up to several times compared to a conventional elution method, when a 0.254 mmID, 15.2âcm connection tubing was used. Increase in plate counts of up to 50-70% was possible for solutes with retention factors smaller than 1.0 for the columns connected with a 0.13âmmID, 15âcm tube. The method also seems to reduce the contribution of the void space at the column inlet to the band dispersion. The elution method including a slow transport of the sample band in the pre-column space of 10 μL or less may require a little longer separation time than normal elution, but it was shown to be effective for increasing the observed efficiency of a small column for solutes with small retention factors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1572, 19 October 2018, Pages 44-53
Journal: Journal of Chromatography A - Volume 1572, 19 October 2018, Pages 44-53
نویسندگان
Ikuma Kuroda, Hideyuki Uzu, Shota Miyazaki, Masayoshi Ohira, Nobuo Tanaka,