کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10156873 | 1666435 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydration dynamics of collagen in aqueous buffer solution as studied by time domain dielectric spectroscopy
ترجمه فارسی عنوان
دینامیک هیدراتاسیون کلاژن در محلول بافر آبی که به وسیله طیف سنجی دی الکتریک زمان محاسبه شده است
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کلمات کلیدی
کلاژن، آب هیدراتاسیون، بازه زمانی اندازه گیری حوزه آرامش دی الکتریک، مجاورت مجتمع، طرح کول کول، پارامترهای ترمودینامیکی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Dielectric relaxation studies of collagen in aqueous buffer solution (pHâ¯3.7) have been carried out at four different temperatures 283, 288, 293 and 298â¯K as a function of collagen concentration between 0.033â¯Î¼M and 0.2â¯Î¼M in the frequency range of 1â¯GHz to 25â¯GHz using time domain dielectric spectroscopy technique. Apart from the dominant mode at high frequency around 14â¯GHz which is assigned to the free water relaxation; we have detected one more pronounced peak close to 1.8â¯GHz in the δ-dispersion range of the loss spectrum of collagen in aqueous buffer solution. The peak around 1.8â¯GHz is attributed to hydration water reorientation of the collagen macromolecules and has obtained detailed information on its temperature and concentration dependence. The retardation imposed on the hydration water by collagen is found to be â4.7, since collagen can cause long range perturbations beyond the first hydration shell. The activation enthalpy and activation entropy for the dipolar orientation for collagen at different concentrations have been calculated from the Arrhenius plot and is found to be 32â¯kJ/mol and 4.2â¯J/molâ¯K respectively. The increase of thermodynamic activation enthalpy and decrease of activation entropy of collagen in buffer in the present study compared to the water have supported the idea that the water associated with collagen is highly ordered.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 118, Part B, 15 October 2018, Pages 1811-1816
Journal: International Journal of Biological Macromolecules - Volume 118, Part B, 15 October 2018, Pages 1811-1816
نویسندگان
R. Sampathkumar, D. Gopalakrishnan, A.C. Kumbharkhane,