کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10159103 | 36 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellular uptake of multilayered capsules produced with natural and genetically engineered biomimetic macromolecules
ترجمه فارسی عنوان
جذب سلولی از کپسول های چند لایه تولید شده با ماکرومولکول های بیومیتی طبیعی و ژنتیکی مهندسی شده
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کلمات کلیدی
جذب سلولی، کپسولهای چند لایه، لایه لایه، بیومارکرهای،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Multilayered microcapsules of chitosan and biomimetic elastin-like recombinamers (ELRs) were prepared envisaging the intracellular delivery of active agents. Two ELRs containing either a bioactive RGD sequence or a scrambled non-functional RDG were used to construct two types of functionalized polymeric microcapsules, both of spherical shape â¼4 μm in diameter. Cell viability studies with human mesenchymal stem cells (hMSCs) were performed using microcapsule/cell ratios between 5:1 and 100:1. After 3 and 72 h of co-incubation, no signs of cytotoxicity were found, but cells incubated with RGD-functionalized microcapsules exhibited higher viability values than RDG cells. The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo. The data show that surface functionalization did not significantly influence internalization by hMSCs, but the bioavailability of DQ-ovalbumin degraded faster when encapsulated within RGD-functionalized microcapsules. The microcapsules developed show promise for intracellular drug delivery with increased drug efficacy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Biomaterialia - Volume 10, Issue 6, June 2014, Pages 2653-2662
Journal: Acta Biomaterialia - Volume 10, Issue 6, June 2014, Pages 2653-2662
نویسندگان
Rui R. Costa, Alessandra Girotti, Mercedes Santos, F. Javier Arias, João F. Mano, J. Carlos RodrÃguez-Cabello,